E-ERAD-238 - Reprogramming around an epigenetic barrier with TET1

Status
Released on 28 April 2014, last updated on 29 January 2015
Organism
Mus Musculus
Samples (47)
Protocols (2)
Description
Mouse Epiblast Stem Cells (EpiSCs) were reprogrammed to induced pluripotent stem cells (iPSCs) by overexpression of key pluripotency factors and the TET1 DNA hydroxylase. 0.2ug of extractd DNA was sonicated using the Covaris Sonicator (300bp) and the Illumina library was prepared following a standard Illumina protocol with the NEB Next kit and subjected to bisulfite treament. The library was amplified 12x and fragments smaller than 200 bp were removed by PEG precipitation on magnetic beads. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
Experiment type
methylation profiling by high throughput sequencing 
Contact
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-ERAD-238.idf.txt
Sample and data relationshipE-ERAD-238.sdrf.txt
Links