E-ERAD-11 - MBD-Seq of human samples to explore functional characterisation of CpG islands. EGA study.
Released on 21 January 2014, last updated on 1 May 2014
Tissue-specific methylation patterns suggest a role for CpG island (CGI) methylation in differentiation and cell-type-specific gene regulation. We have applied CXXC affinity purification (CAP), MBD affinity purification (MAP) and chromatin immunoprecipitation (ChIP) in combination with solexa sequencing to investigate the extent and functional significance of CGI methylation in sperm, blood, cerebellum and ES cells for both human and mouse.
methylation profiling by high throughput sequencing, methodological variation design, organism part comparison design