E-EMBL-3 - Transcription profiling of human gene expression profiles of CD34+/CD38- cells vs AFT024
Released on 13 June 2005, last updated on 1 May 2014
Homo sapiens, Mus musculus
It is widely accepted that hematopoietic progenitor cells (HPC) are tightly associated with discrete niches within the bone marrow. This molecular environment supports and regulates their self renewal and differentiation. AFT024 is a cell line derived from murine fetal liver that has been demonstrated to maintain human hematopoietic progenitors in an undifferentiated state in vitro. While various functional and genomic studies of this and other stromal layers have already been reported, the influence of the cellular microenvironment on the gene expression of HPC has not yet been systematically analyzed.
The CD34+/CD38- fraction of human umbilical cord blood was parted and either cultivated on AFT024 [kindly provided by I. Lemischka] or without stromal feeder layer for 16h, 20h, 48h or 72h. Both fractions were then harvested and separated by vigorous pipetting and FACS sorted again to separate the HPC from AFT024. Global gene expression profiles were determined using a novel Human Genome cDNA Microarray of over 51,145 ESTs of the UnigenSet-RZPD3. In analogy, we have compared the gene expression profiles of CD34+/CD38- cells versus AFT024 to exclude that differential gene expression resulted from contaminating feeder layer cells.
transcription profiling by array, growth condition
Hematopoietic Progenitor Cells and Cellular Microenvironment: Behavioral and Molecular Changes upon Interaction. Wagner, Wolfgang; Saffrich, Rainer; Wirkner, Ute; Eckstein, Volker; Blake, Jonathon; Ansorge, Alexandra; Schwager, Christian; Wein, Frederik; Miesala, Katrin; Ansorge, Wilhelm; Ho, Anthony D. Stem Cells 23(8):1180 (2005), Europe PMC 15955826