E-CBIL-44 - Transcription profiling of mouse wild type and Nkx2.2 mutant embryos at E12.5 and E13.5 to identify a transcripional program that Nkx2.2 regulation in the pancreas
Released on 5 November 2009, last updated on 2 June 2014
The goal of this experiment was to analyze expression changes in the pancreas at embryonic days 12.5 and 13.5 between wild type and Nkx2.2 null mice. We know that Nkx2.2 is essential for pancreatic endocrine differentiation and development. At these early time points which are critical for endocrine cell specification, we would like to identify a transcriptional program that Nkx2.2 regulates. We would also like to identify direct and functional transcriptional targets of Nkx2.2.
transcription profiling by array, genetic modification
Identification of known and novel pancreas genes expressed downstream of Nkx2.2 during development. Keith R Anderson, Peter White, Klaus H Kaestner and Lori Sussel.