E-CBIL-26 - Transcription profiling of mouse brown preadipocytes from wild type and IRS knock-out cell lines
Submitted on 31 January 2007, released on 6 April 2007, last updated on 27 March 2012
Brown preadipocytes were grown to confluence and synchronized by overnight serum starvation. Four independent RNA samples were analyzed from each IRS KO cell line and three independent clones of WT cells were separately analyzed as controls. Splitting of the samples resulted in a total 28 microarrays. 15 mg of adjusted cRNA were hybridized to Affymetrix U74A-v2 arrays.
transcription profiling by array, genetic modification
Prediction of preadipocyte differentiation by gene expression reveals role of insulin receptor substrates and necdin. Yu-Hua Tseng, Atul J Butte, Efi Kokkotou, Vijay K Yechoor, Cullen M Taniguchi, Kristina M Kriauciunas, Aaron M Cypess, Michio Niinobe, Kazuaki Yoshikawa, Mary Elizabeth Patti, C Ronald Kahn. Nat Cell Biol 7(6):601-11 (2005)