E-CBIL-26 - Transcription profiling of mouse brown preadipocytes from wild type and IRS knock-out cell lines

Status
Released on 6 April 2007, last updated on 31 July 2014
Organism
Mus musculus
Samples (7)
Array (1)
Protocols (4)
Description
Brown preadipocytes were grown to confluence and synchronized by overnight serum starvation. Four independent RNA samples were analyzed from each IRS KO cell line and three independent clones of WT cells were separately analyzed as controls. Splitting of the samples resulted in a total 28 microarrays. 15 mg of adjusted cRNA were hybridized to Affymetrix U74A-v2 arrays.
Experiment types
transcription profiling by array, genetic modification
Contact
Citation
Prediction of preadipocyte differentiation by gene expression reveals role of insulin receptor substrates and necdin. Tseng Yu-Hua, Butte Atul J, Kokkotou Efi, Yechoor Vijay K, Taniguchi Cullen M, Kriauciunas Kristina M, Cypess Aaron M, Niinobe Michio, Yoshikawa Kazuaki, Patti Mary Elizabeth, Kahn C Ronald. , Europe PMC 15895078
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-CBIL-26.idf.txt
Sample and data relationshipE-CBIL-26.sdrf.txt
Raw data (1)E-CBIL-26.raw.1.zip
Processed data (1)E-CBIL-26.processed.1.zip
Array designA-AFFY-6.adf.txt
Links