E-CBIL-19 - Transcription profiling of muscle insulin receptor knock-out mice treated with streptozotocin to induce diabetes
Released on 24 November 2006, last updated on 27 March 2012
The targeted muscle insulin receptor knockout (MIRKO) model was used, in which there is a complete absence of the insulin-receptor signaling in skeletal muscle but normal insulin and glucose levels. By comparing skeletal muscle gene-expression profiles from MIRKO mice and their controls (lox/lox) under three different metabolic conditions (namely, in the basal state, after streptozotocin (STZ)-induced diabetes, and after STZ-induced diabetes rendered euglycemic with insulin treatment), we can address the following three important questions. (i) What is the direct effect of the loss of insulin signaling on gene expression in skeletal muscle? (ii) What is the contribution of the metabolic and other changes that accompany diabetes to induce indirect changes in gene expression? (iii) How are these pathways regulated and implicated in the pathophysiology of diabetes?
transcription profiling by array, compound treatment, genetic modification
Distinct pathways of insulin-regulated versus diabetes-regulated gene expression: an in vivo analysis in MIRKO mice. Yechoor Vijay K, Patti Mary-Elizabeth, Ueki Kohjiro, Laustsen Palle G, Saccone Robert, Rauniyar Ravi, Kahn C Ronald.