E-CBIL-15 - Transcription profiling of mouse pancreatic duct cell lines infected with Ngn3-expressing adenovirus

Status
Submitted on 16 June 2006, released on 25 July 2006, last updated on 1 May 2014
Organism
Human adenovirus 5, Mus musculus
Samples (3)
Array (1)
Protocols (7)
Description
We have developed an in vitro model of Ngn3-dependent differentiation by infecting pancreatic duct cell lines with an Ngn3-expressing adenovirus. We used glass microarrays containing 18,000 mouse cDNAs and compared gene expression patterns in mPAC L20 cells infected with either AdCMV-gal (control) or AdCMV-ngn3. All comparisons were performed 48 h after viral infection, meaning that genes differentially expressed in Ngn3-expressing mPAC cells could include genes whose expression was directly or indirectly controlled by Ngn3.
Experiment types
transcription profiling by array, genetic modification
Contacts
Citation
Proendocrine genes coordinate the pancreatic islet differentiation program in vitro. Gasa R, Mrejen C, Leachman N, Otten M, Barnes M, Wang J, Chakrabarti S, Mirmira R, German M. Proc Natl Acad Sci U S A 101(36):13245-50 (2004), Europe PMC 15340143
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-CBIL-15.idf.txt
Sample and data relationshipE-CBIL-15.sdrf.txt
Raw data (1)E-CBIL-15.raw.1.zip
Experiment designE-CBIL-15.biosamples.png, E-CBIL-15.biosamples.svg
Array designA-CBIL-8.adf.txt
Links