E-BUGS-146 - Transcription profiling by array of overexpression of the Rv0805 phosphodiesterase elicits a cAMP-independent transcriptional response

Status
Released on 2 November 2013, last updated on 26 June 2014
Organism
Mycobacterium tuberculosis
Samples (6)
Array (1)
Protocols (3)
Description
The Rv0805 gene in Mycobacterium tuberculosis encodes a metallophosphoesterase which shows cAMP-hydrolytic activity. Overexpression of Rv0805 has been used as a tool to lower intracellular cAMP levels and thereby elucidate the roles of cAMP in mycobacteria. Here we show that levels of cAMP in M. tuberculosis were lowered by only .30% following overexpression of Rv0805, and transcript levels of a number of genes, which include those associated with virulence and the methyl citrate cycle, were altered. The genes that showed altered expression were distinct from those differentially regulated in a strain deleted for the cAMP-receptor protein (CRP(Mt)), consistent with the relatively low dependence on cAMP of CRP(Mt) binding to DNA. Using mutants of Rv0805 we show that the transcriptional signature of Rv0805 overexpression is a combination of catalysis-dependent and independent effects, and that the structurally flexible C-terminus of Rv0805 is crucial for the catalysis-independent effects of the protein. Our study demonstrates the dissociation of Rv0805 and cAMP-regulated gene expression, and reveals alternate functions for this phosphodiesterase from M. tuberculosis. [Data is also available from http://bugs.sgul.ac.uk/E-BUGS-146]
Experiment types
transcription profiling by array, strain or line design
Contact
Citation
Overexpression of the Rv0805 phosphodiesterase elicits a cAMP-independent transcriptional response. Matange N, Hunt DM, Buxton RS, Visweswariah SS. Tuberculosis (Edinb) 93(5):492-500 (2013), Europe PMC 23835087
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-BUGS-146.idf.txt
Sample and data relationshipE-BUGS-146.sdrf.txt
Raw data (1)E-BUGS-146.raw.1.zip
Array designA-BUGS-23.adf.txt
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