A-MAXD-3 - Hiscock-Senecio-Array1
Spotting Method: cDNA inserts were PCR amplified, ethanol precipitated and resuspended in 1xGSSA spotting solution (Genetix Ltd.) prior to transfer to 384-well plates (V-bottom, Greiner) for use in array printing. Inserts were printed onto Amersham CodeLink slides in double-spot format using a BioRobotics MicroGrid II arraying robot. Printed slides were placed in a salt chamber for 24hr before being stored in dry, dust-free conditions until required for hybridisation.
Adhesion Method: Amine linking
Number Of Plates: 18
Array format for studies of allopolyploid origin of Senecio cambrensis. Contains ~1100 clones from each of S. squalidus/S. cambrensis/S. vulgaris capitulum bud and S. squalidus/S. cambrensis/S. vulgaris mature flower bud cDNA libraries. Features were primarily chosen anonymously, so sequence information will be filled in as target clones are identified and sequenced.
Hiscock Plant Reproductive Biology Group, University of Bristol
|All experiments done using A-MAXD-3: (E-MAXD-5, E-MAXD-7)|