A-MAXD-23 - MAXD Birmingham-Stickleback-PGPS2
The stickleback PGP 'S2' microarray was made from amplified cDNA clones of Gasterosteus aculeatus (Three-spined stickleback). These were derived from one whole-body cDNA library (Kingsley et al., (2004) Behaviour 141, 1331-1344.) supplemented by hepatic clones derived from SSH and individually cloned genes (Geoghegan et al., J. Fish Biol. Submitted) and clones from one subtractive and two normalised liver cDNA libraries (Brown et al., Mar. Env. Res. In Press). This array was produced as part of NERC Post Genomics and Proteomics grant grant NE/C507661/1, 'Identifying and defining the bases of individual and population susceptibility and adaptation to environmental pollutants in fish: An integrated "omic" approach'.
Availability: Contact submitter
Spotting Method: ). Briefly, inserts were amplified by PCR from bacterial cultures of stickleback clones in plasmid vectors using vector primers, purified (BD Biosciences Nucleofast 96), transferred to 38x 384 well plates (Genetix) and printed on Corning Ultra-GAPS microarray slides by a Biorobotics MGII arrayer at Birmingham Functional Genomics Laboratory. The stickleback 'S2' microarray consists of 14,496 cDNA clones spotted in duplicate, including control DNA (Geoghegan) and spotting buffer (Corning Pronto) controls.
Adhesion Method: baked 2hr 80C
Number Of Plates: 38
Birmingham University Functional Genomics Laboratory
|All experiments done using A-MAXD-23: (E-MAXD-42, E-MAXD-43)|