A-MAXD-21 - MAXD Birmingham-Stickleback-S1
Stickleback PCR amplicons printed on Corning Ultra-GAPS slides.
Availability: Contact submitter
Spotting Method: PCR products were purified (BD Biosciences Nucleofast 96) and robotically transferred to 27x 384-well plates (Genetix) before re-suspension in Pronto spotting buffer (Corning). This set consisted of 9692 stickleback clones with remaining wells filled by spotting buffer. An additional 384-well plate was added comprising Lucidea calibration, ratio, utility and negative controls (Amersham), plasmid DNA and polylinker amplicons from pRL (Promega), pBluescriptII SK+ (Stratagene), pCR2.1 (Invitrogen) and pTriplEx2 (BD Biosciences) and Renilla luciferase and beta-lactamase amplicons. . Microarray slides (Corning Ultra-GAPS) were printed at Birmingham Functional Genomics Laboratory with an MGII robot (Biorobotics) employing a 48x split-pin tool. Each amplicon was printed in duplicate, for a total of 21600 features. Slides were baked at 80oC 2h post-printing.
Adhesion Method: baked 2hr 80C
Birmingham University Functional Genomics Laboratory