A-MAXD-11 - Hiscock Senecio species 14K Array2
Array format for studies of homoploid origin of Senecio squalidus. Contains ~1100 clones from each of S. squalidus/S. aethnensis/S. chrysanthemifolius capitulum bud and S. squalidus/S. aethnensis/S. chrysanthemifolius mature flower bud cDNA libraries. Features were primarily chosen anonymously, so sequence information will be filled in as target clones are identified and sequenced.
Spotting Method: cDNA inserts were PCR amplified, ethanol precipitated and resuspended in 1xGSSA spotting solution (Genetix Ltd.) prior to transfer to 384-well plates (V-bottom, Greiner) for use in array printing. Inserts were printed onto Amersham CodeLink slides in double-spot format using a BioRobotics MicroGrid II arraying robot. Printed slides were placed in a salt chamber for 24hr before being stored in dry, dust-free conditions until required for hybridisation.
Adhesion Method: Amine linking
Number Of Plates: 18
Hiscock Plant Reproductive Biology Group, University of Bristol