Table of Contents
MUSCLE (MUltiple Sequence Comparison by Log-Expectation) 1) is claimed to achieve both better average accuracy and better speed than ClustalW or T-Coffee, depending on the chosen options. Multiple alignments of protein sequences are important in many applications, including phylogenetic tree estimation, secondary structure prediction and critical residue identification.
For more information see:
Web service registry entries:
There is currently a limit of 500 sequences and 1MB of data.
Sample clients are provided for a number of programming languages. For details of how to use these clients, download the client and run the program without any arguments.
For further details of these tool-kits and workflow platforms see our Guide to Web Services.
The WSDL for the MUSCLE (SOAP) service: http://www.ebi.ac.uk/Tools/services/soap/muscle?wsdl
The Kissinger Research Group, University of Georgia provide a SAWSDL for the MUSCLE (SOAP) web service, which is annotated using OBIws: http://mango.ctegd.uga.edu/jkissingLab/SWS/Wsannotation/resources/muscle.sawsdl
Get a list of the parameter names.
Returns: a list of strings giving the names of the parameters.
Get details of a specific parameter.
Returns: a wsParameterDetails describing the parameter and its values.
Submit a job to the service.
Returns: a string containing the job identifier (
Get the status of a submitted job.
Returns: a string containing the status.
The values for the status are:
Further details can be found in Synchronous and Asynchronous Access: JDispatcher.
Get the available result types for a finished job.
Returns: a list of wsResultType data structures describing the available result types.
Get the result of a job of the specified type.
Returns: the result data for the specified type, base64 encoded. Depending on the SOAP library and programming language used the result may be returned in decoded form. For some result types (e.g. images) this will be binary data rather than a text string.
The input parameters for the job:
More detailed information about each parameter, including valid values can be obtained using the getParameterDetails(parameterId) operation.
Descriptive information about a tool parameter. Returned by getParameterDetails(parameterId).
Description of a tool parameter value. Used in wsParameterDetails.
Properties of a tool parameter value. Used in wsParameterValue.
Additional parameters passed when requesting a result. See getResult(jobId, type, parameters).
Description of a result type. Returned by getResultTypes(jobId).