On Monday 28th March 2011 the WSPSISearch service, used by these clients, was decommissioned and replaced by the following services:

• PSI-Search (REST)
• PSI-Search (SOAP)

The service documentation and clients below are historical and provided solely for reference purposes.

PSI-Search, similar to PSI-BLAST, combines the Smith-Waterman search algorithm with the PSI-BLAST profile construction strategy to find distantly related protein sequences. Searches are done with SSEARCH, and the selected hits are combined with BLASTPGP to build a position specific scoring matrix (PSSM), which is then used for another search with SSEARCH in the next iteration.

For more information about this tool see:

• EBI Tools:
  • PSI-Search
  • PSI-Search Help

For details of the service used by these clients see WSPSISearch.

To run the Perl client you need to install SOAP::Lite. SOAP::Lite is a collection of Perl modules which provide a simple and lightweight interface to the Simple Object Access Protocol (SOAP) for both client and server applications. This client requires SOAP::Lite version 0.60.

Download the Perl client: psisearch.pl

To get usage information run the program without arguments:

perl psisearch.pl