On Monday 28th March 2011 the WSPSIBlast service, used by these clients, was decommissioned and replaced by the following services:

• PSI-BLAST (REST)
• PSI-BLAST (SOAP)

The service documentation and clients below are historical and provided solely for reference purposes.

BLAST stands for Basic Local Alignment Search Tool.The emphasis of this tool is to find regions of sequence similarity, which will yield functional and evolutionary clues about the structure and function of your novel sequence. Position specific iterative BLAST (PSI-BLAST) refers to a feature of BLAST 2.0 in which a profile is automatically constructed from the first set of BLAST alignments. PSI-BLAST is similar to NCBI-BLAST2 except that it uses position-specific scoring matrices derived during the search, this tool is used to detect distant evolutionary relationships.

For more information about this tool see:

• EBI Tools:
  • PSI-BLAST
  • PSI-BLAST Help

For details of the service used by these clients see WSPSIBlast.

To run the Perl client you need to install SOAP::Lite. SOAP::Lite is a collection of Perl modules which provide a simple and lightweight interface to the Simple Object Access Protocol (SOAP) for both client and server applications. This client requires SOAP::Lite version 0.60.

Download the Perl client: psiblast.pl

To get usage information run the program without arguments:

perl psiblast.pl