On Tuesday 20th September 2011 the WSPhobius service, used by these clients, was decommissioned and replaced by the following services:

• Phobius (REST)
• Phobius (SOAP)

The service documentation and clients below are historical and provided solely for reference purposes.

This client is for prediction of transmembrane topology and signal peptides from the amino acid sequence of a protein.

For more information about this tool see:

• EMBL-EBI Tools:
  • Phobius
  • Phobius Help

For details of the service used by these clients see Phobius Web Service.

The Microsoft .NET runtime is already installed on most MS Windows systems. The Microsoft .NET runtime is available via Microsoft Update or as a download (see http://msdn.microsoft.com/netframework/).

For MacOS X, Linux and other non-Windows systems see Mono or DotGNU.

Download the C# client:

• Executable: wsphobius.exe
• Source code: wsphobius.cs

Run without arguments to get a usage summary:

wsphobius.exe

Download and unzip the library files lib-1.4.zip and add the jars to the Java classpath.

Download the Java client:

• Executable: WSPhobius.jar
• Source code: WSPhobiusClient.java

To get usage information run the client without arguments:

java -Djava.ext.dirs=lib -jar WSPhobius.jar

To run the Perl client you need to install SOAP::Lite. SOAP::Lite is a collection of Perl modules which provide a simple and lightweight interface to the Simple Object Access Protocol (SOAP) for both client and server applications. This client requires SOAP::Lite version 0.60.

Download the Perl client: phobius.pl

To get usage information run without arguments:

perl phobius.pl

Workflow engines such as Taverna and Triana offer a simple way of combining individual web services into complex applications.

A basic workflow showing how to use WSPhobius can be found on myExperiment.