ClustalW is a fully automatic program for global multiple alignment of DNA and protein sequences. The alignment is progressive and considers the sequence redundancy. Trees can also be calculated from multiple alignments.

For more information about this tool see:

• EBI Tools:
  • ClustalW
  • ClustalW Help
  • ClustalW FAQ
• ClustalW Documentation

For details of the service used by these clients see WSClustalW.

The Microsoft .NET runtime is already installed on most MS Windows systems. The Microsoft .NET runtime is available via Microsoft Update or as a download (see http://msdn.microsoft.com/netframework/).

For MacOS X, Linux and other non-Windows systems see Mono or DotGNU.

Download the C# client:

• Executable: wsclustalw.exe
• Source code: wsclustalw.cs

Run without arguments to get a usage summary:

wsclustalw.exe

Download and unzip the library files lib-1.4.zip and add the jars to the Java classpath.

Download the Java client:

• Executable: WSClustalW.jar
• Source code: WSClustalWClient.java

To get usage information run the client without arguments:

java -Djava.ext.dirs=lib -jar WSClustalW.jar

To run the Perl client you need to install SOAP::Lite. SOAP::Lite is a collection of Perl modules which provides a simple and lightweight interface to SOAP both on client and server side. This client requires SOAP::Lite version 0.60.

Download the Perl client: clustalw.pl

To get usage information run without arguments:

perl clustalw.pl