Sequence checksum calculator
Introduction
A program to generate checksums for sequences. The checksums can be CRC32, CRC64-ISO, CRC64-ECMA-182,CRC32-CRC64, MD5, SHA1, SHA2 224-bit, SHA2 256-bit, SHA2 384-bit and SHA2 512-bit.
How to use this tool
Running a tool from the web form is a simple multiple steps process, starting at the top of the page and following the steps to the bottom.
Each tool has at least 2 steps, but most of them have more:
- The first steps are usually where the user sets the tool input (e.g. sequences, databases...)
- In the following steps, the user has the possibility to change the default tool parameters
- And finally, the last step is always the tool submission step, where the user can specify a title to be associated with the results and an email address for email notification. Using the submit button will effectively submit the information specified previously in the form to launch the tool on the server
Note that the parameters are validated prior to launching the tool on the server and in the event of a missing or wrong combination of parameters, the user will be notified directly in the form.
Step 1 - Input Sequences
Input Sequence
Three or more sequences to be aligned can be entered directly into this form. Sequences can be be in GCG, FASTA, EMBL, GenBank, PIR, NBRF, PHYLIP or UniProtKB/Swiss-Prot format. Partially formatted sequences are not accepted. Adding a return to the end of the sequence may help certain applications understand the input. Note that directly using data from word processors may yield unpredictable results as hidden/control characters may be present.
Sequence File Upload
A file containing three or more valid sequences in any format (GCG, FASTA, EMBL, GenBank, PIR, NBRF, PHYLIP or UniProtKB/Swiss-Prot) can be uploaded and used as input for the multiple sequence alignment. Word processor files may yield unpredictable results as hidden/control characters may be present in the files. It is best to save files with the Unix format option to avoid hidden Windows characters.
Sequence Type
Indicates if the sequences are protein or nucleotide (DNA/RNA).
| Type | Abbreviation |
|---|---|
| Protein | protein |
| Nucleic Acid | dna |
Default value is: Protein [protein]
Step 2 - Select Applications
Checksum Methods
A number of different checksum methods for sequences are launched.
| Method Name | Description | Abbreviation |
|---|---|---|
| CRC32 | 32-bit checksum using Cyclic Redundancy Check (CRC32). | crc |
| CRC64-ISO | 64-bit checksum using the Cyclic Redundancy Check defined by ISO (CRC-64-ISO) | spcrc |
| CRC64-ECMA-182 | 64-bit checksum using the alternative Cyclic Redundancy Check defined in ECMA-182 (CRC-64-ECMA-182) | altcrc |
| CRC32-CRC64 | Combination of CRC32 and CRC64 sequence checksums with the sequence length | crcdigest |
| MD5 | 128-bit checksum using Message Digest 5 (MD5) | md |
| SHA1 | 160-bit checksum using Secure Hash Algorithm 1 (SHA-1) | shaa |
| SHA2-224 | 224-bit checksum using Secure Hash Algorithm 2 (SHA-2) | shab |
| SHA2-256 | 256-bit checksum using Secure Hash Algorithm 2 (SHA-2) | shac |
| SHA2-384 | 384-bit checksum using Secure Hash Algorithm 2 (SHA-2) | shad |
| SHA2-512 | 512-bit checksum using Secure Hash Algorithm 2 (SHA-2) | shae |
Default value is: SHA2-512 [shae]
Step 3 - Submission
Job title
It's possible to identify the tool result by giving it a name. This name will be associated to the results and might appear in some of the graphical representations of the results.
Email Notification
Running a tool is usually an interactive process, the results are delivered directly to the browser when they become available. Depending on the tool and its input parameters, this may take quite a long time. It's possible to be notified by email when the job is finished by simply ticking the box "Be notified by email". An email with a link to the results will be sent to the email address specified in the corresponding text box. Email notifications require valid email addresses.
Email Address
If email notification is requested, then a valid Internet email address in the form joe@example.org must be provided. This is not required when running the tool interactively (The results will be delivered to the browser window when they are ready).