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EBI Dbfetch

ID   CT476807; SV 4; linear; genomic DNA; STD; VRT; 3630 BP.
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AC   CT476807;
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DT   05-NOV-2005 (Rel. 85, Created)
DT   13-JAN-2009 (Rel. 99, Last updated, Version 10)
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DE   Zebrafish DNA sequence from clone ZFOS-900A7 in linkage group 18 Contains
DE   part of the gene for a novel protein similar to vertebrate Rho guanine
DE   nucleotide exchange factor (GEF) 17 (ARHGEF17).
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KW   HTG.
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OS   Danio rerio (zebrafish)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Cyprinidae; Danio.
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RN   [1]
RP   1-3630
RA   Phillimore B.;
RT   ;
RL   Submitted (12-JAN-2009) to the INSDC.
RL   Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire, CB10 1SA, UK.
RL   E-mail enquiries: zfish-help@sanger.ac.uk Clone requests:
RL   http://www.sanger.ac.uk/Projects/D_rerio/faqs.shtml#dataeight
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DR   MD5; a4ec309f93f951c2d2816b5491cccd99.
DR   ENA-CON; CT993778.
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CC   -------------- Genome Center
CC   Center: Wellcome Trust Sanger Institute
CC   Center code: SC
CC   Web site: http://www.sanger.ac.uk
CC   Contact: zfish-help@sanger.ac.uk
CC   --------------
CC   
CC   This sequence was finished as follows unless otherwise noted: all regions
CC   were either double-stranded or sequenced with an alternate chemistry or
CC   covered by high quality data (i.e., phred quality >= 30); an attempt was
CC   made to resolve all sequencing problems, such as compressions and repeats;
CC   all regions were covered by at least one subclone; and the assembly was
CC   confirmed by restriction digest, except on the rare occasion of the clone
CC   being a YAC.
CC   
CC   The following abbreviations are used to associate primary accession
CC   numbers given in the feature table with their source databases:
CC   Em:, EMBL; Sw:, SWISSPROT; Tr:, TREMBL; Wp:, WORMPEP;
CC   Information on the WORMPEP database can be found at
CC   http://www.sanger.ac.uk/Projects/C_elegans/wormpep
CC   
CC   Clone-derived Zebrafish pUC subclones occasionally display inconsistency
CC   over the length of mononucleotide A/T runs and conserved TA repeats.
CC   Where this is found the longest good quality representation will be
CC   submitted.
CC   
CC   Any regions longer than 1kb tagged as misc-feature "unsure" are part of
CC   a tandem repeat of more than 10kb in length where it has not been possible
CC   to anchor the base differences between repeat copies.  The region has been
CC   built up based on the repeat element to match the total size of repeat
CC   indicated by restriction digest, but repeat copies may not be in the
CC   correct order and the usual finishing criteria may not apply.
CC   
CC   IMPORTANT: This sequence is not the entire insert of clone ZFOS-900A7. It
CC   may be shorter because we sequence overlapping sections only once, except
CC   for a short overlap.
CC   
CC   During sequence assembly data is compared from overlapping clones. Where
CC   differences are found these are annotated as variations together with a
CC   note of the overlapping clone name. Note that the variation annotation may
CC   not be found in the sequence submission corresponding to the overlapping
CC   clone, as we submit sequences with only a small overlap.
CC   
CC   The true right end of clone CH211-248G20 is at 2000 in this sequence.
CC   The true right end of clone ZFOS-900A7 is at 3630 in this sequence.
CC   
CC   ZFOS-900A7 is from a Zebrafish fosmids library
CC   VECTOR: pFOS1-seq_BamHI
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FH   Key             Location/Qualifiers
FH
FT   source          1..3630
FT                   /organism="Danio rerio"
FT                   /chromosome="18"
FT                   /mol_type="genomic DNA"
FT                   /clone_lib="ZFISHFOS"
FT                   /clone="ZFOS-900A7"
FT                   /db_xref="taxon:7955"
FT   misc_feature    2000
FT                   /note="Clone_right_end: CH211-248G20"
FT   misc_feature    3630
FT                   /note="Clone_right_end: ZFOS-900A7"
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SQ   Sequence 3630 BP; 1028 A; 699 C; 758 G; 1145 T; 0 other;
     cgaggggctc caaaagagct gggcactggg aactcctgct cctcctctga gctggtggtc        60
     tctgtggcca tgtccttcgc caccaccgct tcatcatctg acaaacacac aaacagcatc       120
     actgagagct gatctaagta aaagtagcat attttaaaat aggagcaata ccagcatcat       180
     agatgtgaca tttgcaataa aaactttact tacagagaaa gtatttgtta gcattatatt       240
     gaaacgtctg tttatatttt ccaaaactac taaccacaga gttatgggga taagaaaaat       300
     atctatctgt gaacatgttt tatatatgaa atgaggtaaa taatcatgtg tgatgtgacc       360
     aaaaaagtct gtgaagtcag tatacaacat actttgtaga aattccatta attaaactgc       420
     acaacctaaa agaatcaatg ctcatcaaaa agacgagtca gttttgttta acatttctgc       480
     attgataagg gaaaggcttg gttatggatg tgactgatgt gaaattggca cttctgtgac       540
     ttgaaataca gtataattgc ttgaaaacat tgacagagac atttgagtat tttagcagta       600
     agttaaaata caaatataaa atacaataca caaaaaaacc tagaaagggt ttcgctattt       660
     tggtgaaaac atattatttt tttaatgcaa aggtatgaga atgaaattac tcataagaag       720
     tctcttctgc ggcattacag tttaatttat attcaagaag aaaacagtaa gaacacttaa       780
     aaaaatgttc gctgtttgtt caaactgctt ttgtaaagtg agctaaaacc acaaaatttc       840
     tgtttttttt gttttgtttt ttgtttgggg gcttaattgt tttatgttca attcacttac       900
     atttgtaaaa actattaagt taatttaatc cctttatttt gtcccaaaac aagtcaattg       960
     gacaaaaaca cccaccaaca taacgaaaag gtggtcaatt tgaacagtac acaaggttgc      1020
     atttttttac attgcttaaa gggtcacgaa acaccaaaac acatgtttta agctgttgac      1080
     agtggtatat gtgtcccaca ctgctaaaaa cactattagg acacctatat ttcactaaaa      1140
     agtgtaaatt ggttgttttt gcgttatttc aagcaaattc gtacttccgg tttgaaacga      1200
     atttttgaag ctgcgtcacg gtcatgacat aatagcgtgt attccagcgt gcagactgga      1260
     cgcctgtgcc agagtgtgtc ttattacgtc ttacagtgtg ctgcattaat gcatgagtaa      1320
     ggcttggttc aaaccaatca gcgcgctcta ttgtgcaact tcattaatat tcattactgt      1380
     cacagtgttt agacgacaga gacgccacgt tgtgttggca aaacaagcgt gaagtgttgc      1440
     ttttatagtt tgctgcagtt aagtttcgtt ttcattttct ctctgtgaga gcgcagctgg      1500
     agtcacgtgt ggattaacaa tgtacgcgac gctcgacaac aataacttgc gtgtctaagg      1560
     aggattattg tttacctgag agctgttctc atctgcaaac gctgagatcc agattcgctt      1620
     tagtctcctc ttaataaaaa tgcggctcta gttgctggtg attgtcctgt ctctacagat      1680
     ttggtaagtg agcgaccagt gttttttgtt tattcagttc gtattttatt cgtatcgaac      1740
     aaactattgc accgagtgta aacatgttag caccacaacc aaactttaac cttgtatagg      1800
     attttcaccg caattagtga ccggaataac acacgcggct ttctgcacta cctgccgtgt      1860
     gcatctaagt ttctgggaaa tgctgagttt tttttttctc tcattcgccg tgcggtatca      1920
     aacattgcat gaaaaataca cgcttagagc agttcctcga atcaaatatc tcgtttgtcg      1980
     cgagggacat gaatgaattc cctgaatgaa agagccaaac tgcagttaaa gtccaccatt      2040
     taataatttg gcaaataatt cgactacaga tgtccatgta aacagtcact ttgtcccctg      2100
     tgtgtgtgta ttttgactct gaaactcagc gcacccgcac caagcctctt ttcttcctcc      2160
     gacactcccc cctaaacaga gctggacacg cccacttttc tgactttttc ccaaagtaga      2220
     ggtgtgaaaa caccctgctg aaacgagggg gtttcatggc cctttaagca tttataactt      2280
     ttttttcatt gtgagcgttt taaactctag atgatggata ataaagccct aagtgtcttt      2340
     ttttcaaaga tacataaacc gtgaatgtag gccaaattat tcagcagctg ttactgtttt      2400
     agtttggaaa ggtcagtttt gagaaagtga cggtgtgaga gaaaaggaca ccgatatagt      2460
     cttatataaa ctttgcacag aaatcttatt ttgcatgcta tcttcatcaa atttgaaata      2520
     taaactcatt tgaatgcata ttttttctag gtctttacat gttttgtttt atttggaaaa      2580
     acaaatattg gacacagtac aatttttctt tctcattact tcataatgta taacttttca      2640
     tattttttag catgaaactt taagtttaaa gtgtcttctt tccaatgatg cctaatttat      2700
     gcttgtagct cactggggtg aagaactgct actatttaaa tttaggtagg tgtaaacccc      2760
     caaaagtgag tgctggctaa caggttcagc tgcttctttg ctggtcaggg ctaggctggc      2820
     tgtgtgaatc ctacctaaca agtgtactca gtttttgttc agcaggggac tttactatct      2880
     aggtttattt aataataata attttaaaca gcatgttttt gtagactcac tgccgaagct      2940
     ggatgatatg gtggagtgtg aggcctggct ttgcagtgtt gatgaagggc tgggcgagtc      3000
     ctcatcgtca gtgtcgtcgc tgtcgaaggg cacgagttct gcagtgggca ccgctggcgg      3060
     ctcgctgagc tccagagacg agccagagat gcagatgtgg agctgtggtt gagttgtcgc      3120
     tgcagagttt gaatctgatg gagccgtcgg tgtggagtga accacacgct ctctgaagga      3180
     gacacaatga acatttcgga ctattcacaa gcatgcaaaa agtacaggcc cataggcagc      3240
     ctggctgggg tgcttcttgt attgtttaaa cctatgcggt cttcattggg tcacacttgt      3300
     aaaccatgta ggttgaaatg tccaattaaa tgtacctttt gagatctcaa gtggaagtgt      3360
     gcttactttt ttgttattgt ttttgttgtt tgtgtgtttt tctcatgatc tgacctgttc      3420
     ttgagtcccg gaacagtggc gatgcagatg atgcgagcgg agcagacggc gatgcaggcc      3480
     tccaccgtgg gctcgtcccg gatgttcagc aggcacacct gaccaacgta accatcgctg      3540
     ttacacaccc acacctcata cgtgttctcc gggcagctgt ggctgggaga cgcacaggaa      3600
     aactgcatcc cgctgcgcgt cttcatgatc                                       3630
//


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