Dbfetch
ID BC082263; SV 1; linear; mRNA; STD; HUM; 1480 BP. XX AC BC082263; XX DT 11-SEP-2004 (Rel. 81, Created) DT 15-OCT-2008 (Rel. 97, Last updated, Version 4) XX DE Homo sapiens choline kinase beta, mRNA (cDNA clone MGC:99491 DE IMAGE:6384695), complete cds. XX KW MGC. XX OS Homo sapiens (human) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; OC Homo. XX RN [1] RP 1-1480 RX DOI; 10.1073/pnas.242603899. RX PUBMED; 12477932. RG Mammalian Gene Collection Program Team RA Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D., RA Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F., RA Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., Hopkins R.F., Jordan H., RA Moore T., Max S.I., Wang J., Hsieh F., Diatchenko L., Marusina K., RA Farmer A.A., Rubin G.M., Hong L., Stapleton M., Soares M.B., Bonaldo M.F., RA Casavant T.L., Scheetz T.E., Brownstein M.J., Usdin T.B., Toshiyuki S., RA Carninci P., Prange C., Raha S.S., Loquellano N.A., Peters G.J., RA Abramson R.D., Mullahy S.J., Bosak S.A., McEwan P.J., McKernan K.J., RA Malek J.A., Gunaratne P.H., Richards S., Worley K.C., Hale S., Garcia A.M., RA Gay L.J., Hulyk S.W., Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., RA Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., RA Sanchez A., Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G., RA Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C., Rodriguez A.C., RA Grimwood J., Schmutz J., Myers R.M., Butterfield Y.S., Krzywinski M.I., RA Skalska U., Smailus D.E., Schnerch A., Schein J.E., Jones S.J., Marra M.A.; RT "Generation and initial analysis of more than 15,000 full-length human and RT mouse cDNA sequences"; RL Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002). XX RN [2] RC NIH-MGC Project URL: http://mgc.nci.nih.gov RP 1-1480 RG NIH MGC Project RA ; RT ; RL Submitted (03-SEP-2004) to the INSDC. RL National Institutes of Health, Mammalian Gene Collection (MGC), Bethesda, RL MD 20892-2590, USA XX DR MD5; 136d53f7f8d4c9ffb3840690ed44b3d8. DR Ensembl-Gn; ENSG00000100288; homo_sapiens. DR Ensembl-Tr; ENST00000406938; homo_sapiens. XX CC Contact: MGC help desk CC Email: cgapbs-r@mail.nih.gov CC Tissue Procurement: DCTD/DTP/Gazdar CC cDNA Library Preparation: Rubin Laboratory CC cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) CC DNA Sequencing by: National Institutes of Health Intramural CC Sequencing Center (NISC), CC Gaithersburg, Maryland; CC Web site: http://www.nisc.nih.gov/ CC Contact: nisc_mgc@nhgri.nih.gov CC Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B., CC Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S., CC Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P., CC Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R., CC Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C., CC McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W., CC Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L., CC Young,A., Zhang,L.-H. and Green,E.D. CC Clone distribution: MGC clone distribution information can be found CC through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov CC Series: IRAL Plate: 59 Row: l Column: 13 CC This clone was selected for full length sequencing because it CC passed the following selection criteria: matched mRNA gi: 23238259. CC Differences found between this sequence and the human reference CC genome (build 36) are described in misc_difference features below. XX FH Key Location/Qualifiers FH FT source 1..1480 FT /organism="Homo sapiens" FT /lab_host="DH10B-R" FT /mol_type="mRNA" FT /clone_lib="NIH_MGC_18" FT /clone="MGC:99491 IMAGE:6384695" FT /tissue_type="Lung, large cell carcinoma" FT /note="Vector: pOTB7" FT /db_xref="taxon:9606" FT gene 1..1480 FT /gene="CHKB" FT /note="synonym: CHETK" FT CDS 56..1243 FT /codon_start=1 FT /gene="CHKB" FT /product="choline kinase beta" FT /db_xref="GOA:Q9Y259" FT /db_xref="H-InvDB:HIT000265978.12" FT /db_xref="HGNC:HGNC:1938" FT /db_xref="InterPro:IPR011009" FT /db_xref="InterPro:IPR026712" FT /db_xref="PDB:2IG7" FT /db_xref="PDB:3FEG" FT /db_xref="PDB:3LQ3" FT /db_xref="UniProtKB/Swiss-Prot:Q9Y259" FT /protein_id="AAH82263.1" FT /translation="MAAEATAVAGSGAVGGCLAKDGLQQSKCPDTTPKRRRASSLSRDA FT ERRAYQWCREYLGGAWRRVQPEELRVYPVSGGLSNLLFRCSLPDHLPSVGEEPREVLLR FT LYGAILQGVDSLVLESVMFAILAERSLGPQLYGVFPEGRLEQYIPSRPLKTQELREPVL FT SAAIATKMAQFHGMEMPFTKEPHWLFGTMERYLKQIQDLPPTGLPEMNLLEMYSLKDEM FT GNLRKLLESTPSPVVFCHNDIQEGNILLLSEPENADSLMLVDFEYSSYNYRGFDIGNHF FT CEWVYDYTHEEWPFYKARPTDYPTQEQQLHFIRHYLAEAKKGETLSQEEQRKLEEDLLV FT EVSRYALASHFFWGLWSILQASMSTIEFGYLDYAQSRFQFYFQQKGQLTSVHSSS" FT misc_difference 1377 FT /gene="CHKB" FT /note="'C' in cDNA is 'T' in the human genome." FT misc_difference 1459..1480 FT /gene="CHKB" FT /note="polyA tail: 22 bases do not align to the human FT genome." XX SQ Sequence 1480 BP; 335 A; 420 C; 431 G; 294 T; 0 other; cccgtccgaa gggagcggag cgcagcctgg cctggggccc ggtcgagccc gcgccatggc 60 ggccgaggcg acagctgtgg ccggaagcgg ggctgttggc ggctgcctgg ccaaagacgg 120 cttgcagcag tctaagtgcc cggacactac cccaaaacgg cggcgcgcct cgtcgctgtc 180 gcgtgacgcc gagcgccgag cctaccaatg gtgccgggag tacttgggcg gggcctggcg 240 ccgagtgcag cccgaggagc tgagggttta ccccgtgagc ggaggcctca gcaacctgct 300 cttccgctgc tcgctcccgg accacctgcc cagcgttggc gaggagcccc gggaggtgct 360 tctgcggctg tacggagcca tcttgcaggg cgtggactcc ctggtgctag aaagcgtgat 420 gttcgccata cttgcggagc ggtcgctggg gccccagctg tacggagtct tcccagaggg 480 ccggctggaa cagtacatcc caagtcggcc attgaaaact caagagcttc gagagccagt 540 gttgtcagca gccattgcca cgaagatggc gcaatttcat ggcatggaga tgcctttcac 600 caaggagccc cactggctgt ttgggaccat ggagcggtac ctaaaacaga tccaggacct 660 gcccccaact ggcctccctg agatgaacct gctggagatg tacagcctga aggatgagat 720 gggcaacctc aggaagttac tagagtctac cccatcgcca gtcgtcttct gccacaatga 780 catccaggaa gggaacatct tgctgctctc agagccagaa aatgctgaca gcctcatgct 840 ggtggacttc gagtacagca gttataacta taggggcttt gacattggga accatttttg 900 tgagtgggtt tatgattata ctcacgagga atggcctttc tacaaagcaa ggcccacaga 960 ctaccccact caagaacagc agttgcattt tattcgtcat tacctggcag aggcaaagaa 1020 aggtgagacc ctctcccaag aggagcagag aaaactggaa gaagatttgc tggtagaagt 1080 cagtcggtat gctctggcat cccatttctt ctggggtctg tggtccatcc tccaggcatc 1140 catgtccacc atagaatttg gttacttgga ctatgcccag tctcggttcc agttctactt 1200 ccagcagaag gggcagctga ccagtgtcca ctcctcatcc tgactccacc ctcccactcc 1260 ttggatttct cctggagcct ccagggcagg accttggagg gaggaacaac gagcagaagg 1320 ccctggcgac tgggctgagc ccccaagtga aactgaggtt caggagaccg gcctgtccct 1380 gagtttgagt aggtccccat ggctggcagg ccagagcccc gtgctgtgta tgtaacacaa 1440 taaacaagct tcttcttcaa aaaaaaaaaa aaaaaaaaaa 1480 //