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EBI Dbfetch

ID   BC055055; SV 1; linear; mRNA; STD; MUS; 6000 BP.
XX
AC   BC055055;
XX
DT   17-JUL-2003 (Rel. 76, Created)
DT   24-SEP-2008 (Rel. 97, Last updated, Version 7)
XX
DE   Mus musculus carbohydrate (chondroitin 6/keratan) sulfotransferase 3, mRNA
DE   (cDNA clone MGC:62836 IMAGE:6493234), complete cds.
XX
KW   MGC.
XX
OS   Mus musculus (house mouse)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea;
OC   Muridae; Murinae; Mus; Mus.
XX
RN   [1]
RP   1-6000
RX   DOI; 10.1073/pnas.242603899.
RX   PUBMED; 12477932.
RG   Mammalian Gene Collection Program Team
RA   Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D.,
RA   Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F.,
RA   Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., Hopkins R.F., Jordan H.,
RA   Moore T., Max S.I., Wang J., Hsieh F., Diatchenko L., Marusina K.,
RA   Farmer A.A., Rubin G.M., Hong L., Stapleton M., Soares M.B., Bonaldo M.F.,
RA   Casavant T.L., Scheetz T.E., Brownstein M.J., Usdin T.B., Toshiyuki S.,
RA   Carninci P., Prange C., Raha S.S., Loquellano N.A., Peters G.J.,
RA   Abramson R.D., Mullahy S.J., Bosak S.A., McEwan P.J., McKernan K.J.,
RA   Malek J.A., Gunaratne P.H., Richards S., Worley K.C., Hale S., Garcia A.M.,
RA   Gay L.J., Hulyk S.W., Villalon D.K., Muzny D.M., Sodergren E.J., Lu X.,
RA   Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S.,
RA   Sanchez A., Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G.,
RA   Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C., Rodriguez A.C.,
RA   Grimwood J., Schmutz J., Myers R.M., Butterfield Y.S., Krzywinski M.I.,
RA   Skalska U., Smailus D.E., Schnerch A., Schein J.E., Jones S.J., Marra M.A.;
RT   "Generation and initial analysis of more than 15,000 full-length human and
RT   mouse cDNA sequences";
RL   Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002).
XX
RN   [2]
RC   NIH-MGC Project URL: http://mgc.nci.nih.gov
RP   1-6000
RG   NIH MGC Project
RA   ;
RT   ;
RL   Submitted (11-JUL-2003) to the INSDC.
RL   National Institutes of Health, Mammalian Gene Collection (MGC), Bethesda,
RL   MD 20892-2590, USA
XX
DR   MD5; a8b38f353318659f162f17fdeb0b3027.
DR   Ensembl-Gn; ENSMUSG00000057337; mus_musculus.
DR   Ensembl-Tr; ENSMUST00000068690; mus_musculus.
DR   Ensembl-Tr; ENSMUST00000135158; mus_musculus.
DR   Ensembl-Tr; ENSMUST00000167915; mus_musculus.
XX
CC   Contact: MGC help desk
CC   Email: cgapbs-r@mail.nih.gov
CC   Tissue Procurement: The Cepko Laboratory
CC   cDNA Library Preparation: Life Technologies, Inc.
CC   cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
CC   DNA Sequencing by: National Institutes of Health Intramural
CC   Sequencing Center (NISC),
CC   Gaithersburg, Maryland;
CC   Web site: http://www.nisc.nih.gov/
CC   Contact: nisc_mgc@nhgri.nih.gov
CC   Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
CC   Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
CC   Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
CC   Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
CC   Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
CC   McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
CC   Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
CC   Young,A., Zhang,L.-H. and Green,E.D.
CC   Clone distribution: MGC clone distribution information can be found
CC   through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
CC   Series: IRAK Plate: 116 Row: l Column: 16
CC   This clone was selected for full length sequencing because it
CC   passed the following selection criteria: matched mRNA gi: 34328238.
CC   Differences found between this sequence and the mouse C57BL/6J
CC   genome (build 36) are described in misc_difference features below.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..6000
FT                   /organism="Mus musculus"
FT                   /lab_host="DH10B"
FT                   /mol_type="mRNA"
FT                   /clone_lib="NIH_MGC_94"
FT                   /clone="MGC:62836 IMAGE:6493234"
FT                   /tissue_type="Eye, retina, mouse strain C57Bl\6"
FT                   /note="Vector: pCMV-SPORT6"
FT                   /db_xref="taxon:10090"
FT   gene            1..6000
FT                   /gene="Chst3"
FT                   /note="synonyms: C6ST, GST-0"
FT   CDS             496..1914
FT                   /codon_start=1
FT                   /gene="Chst3"
FT                   /product="carbohydrate (chondroitin 6/keratan)
FT                   sulfotransferase 3"
FT                   /db_xref="GOA:O88199"
FT                   /db_xref="InterPro:IPR000863"
FT                   /db_xref="InterPro:IPR016469"
FT                   /db_xref="InterPro:IPR027417"
FT                   /db_xref="MGI:MGI:1858224"
FT                   /db_xref="UniProtKB/Swiss-Prot:O88199"
FT                   /protein_id="AAH55055.1"
FT                   /translation="MEKGLALPQDFRDLVHSLKIRGRYVLFLAFVVIVFIFIEKENKII
FT                   SRVSDKLKQIPHFVADANSTDPALLLSENASLLSLSELDSTFSHLRSRLHNLSLQLGVE
FT                   PAMESQEAGAEKPSQQAGAGTRRHVLLMATTRTGSSFVGEFFNQQGNIFYLFEPLWHIE
FT                   RTVFFQQRGASAAGSALVYRDVLKQLLLCDLYVLEPFISPPPEDHLTQFLFRRGSSRSL
FT                   CEDPVCTPFVKKVFEKYHCRNRRCGPLNVTLAGEACRRKDHVALKAVRIRQLEFLQPLV
FT                   EDPRLDLRVIQLVRDPRAVLASRIVAFAGKYENWKKWLSEGQDQLSEDEVQRLRGNCES
FT                   IRLSAELGLRQPAWLRGRYMLVRYEDVARRPLQKAREMYSFAGIPLTPQVEDWIQKNTQ
FT                   ATRDSSDVYSTQKNSSEQFEKWRFSMPFKLAQVVQAACGPTMHLFGYKLARDAASLTNR
FT                   SISLLEERGTFWVT"
FT   misc_difference 5986..6000
FT                   /gene="Chst3"
FT                   /note="polyA tail: 15 bases do not align to the mouse
FT                   genome."
XX
SQ   Sequence 6000 BP; 1417 A; 1535 C; 1583 G; 1465 T; 0 other;
     cttagccact gaaccatctc tccagcctct gttttggggt gtttgtttga gacagaatca        60
     aaaggctggt ctaaagtaca tattgtagct aaagatggct tccaactcct gccttgcctc       120
     cagagtgtta gggtgtgacc tttaaatgac ctattaaaga aaccccaaca ggtgtagagt       180
     acacctgtga tcccagctct taggaggttg gacagatgga taaagaggtc caggtgtccc       240
     ggactacata gagagaccct atctcaaaag agaggcgaag acttaggttt tgagagtggg       300
     cctgaggtct ctcttcaaaa gttttataga aagatgtctc tgttccctgt ctatgaacac       360
     ggatggcctg agcacctgtc tcttcacagg atcagagtgt cccccacctg aagagggctg       420
     attgggtccc aagctatgct cctgagctga gtgcctgcag ccagtctgag gaactccatg       480
     gcgccccctc tccccatgga gaaaggactc gctttgcctc aggatttccg ggaccttgta       540
     cacagcctaa agattcgagg cagatacgtc ttgttcctgg catttgtggt catagttttt       600
     atcttcattg aaaaggaaaa taaaatcata tccagggtct ccgacaagct gaagcagatc       660
     cctcattttg tggcagatgc caacagcact gacccagccc tgctcttatc ggagaatgca       720
     tctctcttgt ccctgagcga gttggattcc accttttccc atctgcggag ccgcctgcac       780
     aacctgagcc tgcagctggg cgtggagcca gcaatggaga gccaggaggc tggggcagag       840
     aagccatccc agcaggctgg agcagggacc cggcgccacg tgcttctcat ggccaccacc       900
     cgcacgggtt cctcgttcgt gggcgagttc ttcaaccagc agggcaatat cttctacctc       960
     ttcgagccac tgtggcacat cgagcgcacc gtgttcttcc agcagcgagg cgccagcgcg      1020
     gctggttcag ccttggtcta ccgtgatgtc ctcaagcagt tgttgctatg cgacctgtat      1080
     gtgctggagc ccttcatcag ccctccgccc gaggaccact tgactcagtt cctgttccgc      1140
     cggggatcca gccgttcact ctgcgaggat ccggtgtgca cacccttcgt caagaaggtc      1200
     tttgagaagt accactgcag gaaccgtcgc tgcgggccac tcaacgtgac cttggcgggc      1260
     gaggcctgcc gccgcaagga ccacgtggcc ctcaaggctg tgcgcatccg tcagctggag      1320
     ttcctgcagc cgctagttga ggacccgagg ttggatctac gagtcattca gctggtgcgc      1380
     gacccccggg ccgtgctggc ttcacgcata gtggcctttg cgggcaagta tgagaactgg      1440
     aagaagtggc tgtccgaggg gcaggaccag ctgagcgagg atgaggtgca gcgattgcgg      1500
     ggcaactgtg agagcatccg cctgtctgca gagctgggct tgcggcagcc agcctggctg      1560
     cgcggtcgtt acatgctggt gcgctatgag gatgtggcac gcaggccact gcagaaggcc      1620
     cgagagatgt acagctttgc gggcatcccc ttgaccccgc aggtggagga ctggatccag      1680
     aagaacacgc aggcgacacg cgacagcagc gatgtctact ccactcagaa aaactcttct      1740
     gagcagtttg agaagtggcg cttcagcatg cctttcaagc tggcacaggt ggtacaggct      1800
     gcctgtggcc cgaccatgca cctctttggc tacaagttgg ccagggatgc cgcctcactc      1860
     accaaccgct ccatcagcct gctggaggag cggggcacct tctgggtcac gtagtggggg      1920
     atgtctggga cccttggaac tccttcttgt gaaaggctgg ccctgcttcc ctcacaccca      1980
     gcctggcagt gagacatagc cctggcagaa agtcaaaatg gggagcgatg atgggaacat      2040
     agcccctggc tgtagcggta gggccccctg ccagctagac tccccagagc agctacagct      2100
     agggccttgg gctcctctga ggacaccctg tctccttagt ggtactggtc ataaggtgtc      2160
     ctcaccctat gacctgcagt gtcccgagca ggtcaaggta ggttcctgtg tgtggacaca      2220
     cctccctagc tctttctcac acagtctaca cgaagcctgt aaaggcctgt aggttgtgtg      2280
     ggctggagtc caagtattta acaaccagaa ggggtgtaga ccctctgcag cctctcagac      2340
     ctcctactgt attaagtgtt aacctcttcc gctctgagtc agagaagctg ggatctggct      2400
     gagtcctggg aaggaggagg acagcctagg gtgaggaagg ggacctttga agctcctcag      2460
     ggaacagtgg ctgtgtacca gctcatagaa aatggtttga tcagctgttc tagtcactca      2520
     tgagtatcaa tcagcctgtg tagagcaaga cacacaaagt gcattgaaaa cagacaaggc      2580
     caggggtgtg gctcagcggt aaagcccttg cctctctcat gcccagggcc ctaggtgtga      2640
     ttcttagcac tataaatata agggaaaata agccatacat acgtgtgcag tacagagaga      2700
     gacagaaaag cgtaataaac attcccccct ctcataagca cacgctctgc ctctgtctct      2760
     gtctctgtct ctctctgtct ctgtctctct ctgtctctct ctctcactct ctctctctct      2820
     ctctctctct cacacacaca cacacacaca cacacacaaa cacacacaca cacactggta      2880
     cagaaaagct tcccttctcc actctttatg ctcaccatgt ttttaggatt tttgtttgtt      2940
     tgttttgttt tgggtttcta tatatctaaa caaggagcct taaattacat ctttttggga      3000
     ttacgtggcg agggggagca gcagggagca tttgcttctg gtaaaaggat agaccagaaa      3060
     ggacttcccc ttggatgatc ggagtcatgc agagccccca gggtcccaca tgttctgtgt      3120
     gaacttcatg tggaatgact cacagaagtg actaccttag gcgctcgctc tgtagctcaa      3180
     tgtgcgtaga gtttgtctat tgtataggaa gccctgggtt ctgtgccctg ccccacacca      3240
     tataggtatg atggcgtgta cccatatccc agcattcagg aaatcaaagg ttggcggtca      3300
     aaagttcaag gtgcagctat gtatcttttg agatgtccga gtctataagc tccctctact      3360
     gagagctgct agtattcctt ctcaagaaca ggttatttga aaatctcttc ttgtaagccc      3420
     caacatttgg gggaggggag gatgggggaa ctcttgactg agcagctagg tttccccaag      3480
     cagaagttct tctctcccag tgcaagctga ggtttagtgc cccggctagc ctcggaactg      3540
     gctcagtggg ggttggcacg ctgacttcct ggacaattgc tgcatctgac actgtgagcg      3600
     cccaggacac cggatgtgta gcatggatgt gggcctagca caggtgtgga agaggacagt      3660
     caggacactc actgttggtg tttgttatcc acctgttcag ccgccgggtc ccacacatag      3720
     gtcatctttc gtcactcaag ccggcctctg tttttgattt taacaatcca agagcaagtg      3780
     tgtaggggac aaagagacac cagctcttca tacttgatgg agaccgggac aggatgctgc      3840
     aggcaggctc gggagtgcat tatcgtttct tctgagctct tcatccaggc catattccct      3900
     tttctgcatc gcatctgggg tgggaggggc tgagctggga gtttccgtcc ttcttccctg      3960
     tgggggtggg agatggggct caaggtctcc atctctcggc tccctgagga cagaaccccc      4020
     acagtggacc tttgggcctt ctcaggacat tgacaatgtt gtgtgcactg caagttgact      4080
     ttatttattt tgtaggaaaa agagatggta ttctttagca ggatctgaaa ctgtacccta      4140
     gtcaagaagt acaattaata acattattat taataacaat tgggatgatt caaaggtcac      4200
     acacacacca gaccacccaa gaaccatgta ggagatgagg cggagccagg tcatggaagc      4260
     ccagaatgct acagagattc tgtgggtcta ttattgatca gaaaatacaa catggggggg      4320
     ccttttctat ggcataactc aggtgtctgc aaagacagag cagctcaggt gggtggatag      4380
     gcaggcagat ggagccttga atcacatccc ctgaggctca gccagccagt gcgaatcctc      4440
     aaggttcgga aagggacagt gtagcggcaa actactgggc atcttctctg atgcttagag      4500
     gctcttacca aggagcttga tggagaaagt ggccatgttg gtggcatggg acaactgctc      4560
     acccaacttc aaagatcaaa gggcacccag gtggcctatg acagtgacac tctttcctac      4620
     cattaggggt gtctgccccc actgaaagcc atgaaatttc tggccctaag ggggtaggaa      4680
     ggacttagga gtagcagatg gtttgatccc atccccccca cacacacaca cacatccctc      4740
     agctgtcttc cacattagag ccacttcagt tgtccatgga cttgtccctt tgagacatcc      4800
     tggattttga aggatagaaa catccccaag atggtctcgt gttaatccca caacagaggc      4860
     agaagggtca tactgagaga gagagagaga gagagagaga gagagagagg agtgggggcc      4920
     agaccctcaa gaagccaagt gggtctggtc aacctgtgca catgagaagg agggaacatc      4980
     actaaaatca gggcctgggc tggtgtgttg ttggtgagat cccgtggagt ggctggctag      5040
     atatggatga gttttctgag catgctcaca cacccccaac ttcacattct tagaaatagc      5100
     acaaccataa tgccttacct caaaaggatg aggcaaagct tgcaattaat tcccagtgtc      5160
     tgaagacctc cgggatcctt tgagctgtgt gtccttgtca gtacatgggg acaggctcct      5220
     taggtattcc tgacatagag gtaaggtgct gcccttgctg ccttgcataa gctgtgacaa      5280
     gcttcttact gggcacatag aacggctctg tcatctgctt ccactaaatt tgggcttgga      5340
     ctttgcctcc tgccaaatct ccatctctgc tggatagtct agtccctagg gtctaaccac      5400
     caccctccac ttcctggtgg gtcctacaag cgctgtcttt gtgccagtac ccggatggtg      5460
     tcctgcctca tggctaaatg gtacaggaca tcttcccaga ctgagtggtc atggcatgtg      5520
     catgtatgtt catgagtaca tgcatggtgt gtgggggtgg atgggtgcat tctgttctgc      5580
     ttctggtgca gctacacagc cacaacctct ttctgtcatt gaccttcttg gtcttcttgt      5640
     agccacagat aatcttccaa tgcccgattc tgctgttctc atctgagagc tgacaaccca      5700
     gcgctcagag tagagttcat gacctaggaa acccttctcc tggtagctta ctgaacttat      5760
     ttaattaaaa acgaacatat aggggttgga gagatggttc attggttaag agcaccggct      5820
     gcttttgcaa aggacctgag ttcaaatccc agcaaccata tggtggctta caaccatctg      5880
     taatgagttc tggtgccctc ttctggcatg caggtgtaca tgcagataga gcactcctat      5940
     gcataaaata aataaatctt ttaaataaat aaataaatga acatgaaaaa aaaaaaaaaa      6000
//


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