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EBI Dbfetch

ID   BC035927; SV 1; linear; mRNA; STD; MUS; 2265 BP.
XX
AC   BC035927;
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DT   24-SEP-2002 (Rel. 73, Created)
DT   24-SEP-2008 (Rel. 97, Last updated, Version 11)
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DE   Mus musculus solute carrier family 22 (organic anion/cation transporter),
DE   member 12, mRNA (cDNA clone MGC:18370 IMAGE:4242354), complete cds.
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KW   MGC.
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OS   Mus musculus (house mouse)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea;
OC   Muridae; Murinae; Mus; Mus.
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RN   [1]
RP   1-2265
RX   DOI; 10.1073/pnas.242603899.
RX   PUBMED; 12477932.
RG   Mammalian Gene Collection Program Team
RA   Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D.,
RA   Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F.,
RA   Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., Hopkins R.F., Jordan H.,
RA   Moore T., Max S.I., Wang J., Hsieh F., Diatchenko L., Marusina K.,
RA   Farmer A.A., Rubin G.M., Hong L., Stapleton M., Soares M.B., Bonaldo M.F.,
RA   Casavant T.L., Scheetz T.E., Brownstein M.J., Usdin T.B., Toshiyuki S.,
RA   Carninci P., Prange C., Raha S.S., Loquellano N.A., Peters G.J.,
RA   Abramson R.D., Mullahy S.J., Bosak S.A., McEwan P.J., McKernan K.J.,
RA   Malek J.A., Gunaratne P.H., Richards S., Worley K.C., Hale S., Garcia A.M.,
RA   Gay L.J., Hulyk S.W., Villalon D.K., Muzny D.M., Sodergren E.J., Lu X.,
RA   Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S.,
RA   Sanchez A., Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G.,
RA   Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C., Rodriguez A.C.,
RA   Grimwood J., Schmutz J., Myers R.M., Butterfield Y.S., Krzywinski M.I.,
RA   Skalska U., Smailus D.E., Schnerch A., Schein J.E., Jones S.J., Marra M.A.;
RT   "Generation and initial analysis of more than 15,000 full-length human and
RT   mouse cDNA sequences";
RL   Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002).
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RN   [2]
RC   NIH-MGC Project URL: http://mgc.nci.nih.gov
RP   1-2265
RG   NIH MGC Project
RA   ;
RT   ;
RL   Submitted (31-JUL-2002) to the INSDC.
RL   National Institutes of Health, Mammalian Gene Collection (MGC), Bethesda,
RL   MD 20892-2590, USA
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DR   MD5; 1223e3d75115c57c6907a6ac7da9752f.
DR   Ensembl-Gn; ENSMUSG00000061742; mus_musculus.
DR   Ensembl-Tr; ENSMUST00000113451; mus_musculus.
XX
CC   Contact: MGC help desk
CC   Email: cgapbs-r@mail.nih.gov
CC   Tissue Procurement: Jeffrey E. Green, M.D.
CC   cDNA Library Preparation: Life Technologies, Inc.
CC   cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
CC   DNA Sequencing by: Sequencing Group at the Stanford Human Genome
CC   Center, Stanford University School of Medicine, Stanford, CA  94305
CC   Web site:       http://www-shgc.stanford.edu
CC   Contact:  (Dickson, Mark) mcd@paxil.stanford.edu
CC   Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
CC   R. M.
CC   Clone distribution: MGC clone distribution information can be found
CC   through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
CC   Series: IRAK Plate: 25 Row: l Column: 24
CC   This clone was selected for full length sequencing because it
CC   passed the following selection criteria: matched mRNA gi: 31982004.
CC   Differences found between this sequence and the mouse C57BL/6J
CC   genome (build 36) are described in misc_difference features below.
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FH   Key             Location/Qualifiers
FH
FT   source          1..2265
FT                   /organism="Mus musculus"
FT                   /lab_host="DH10B"
FT                   /strain="FVB/N"
FT                   /mol_type="mRNA"
FT                   /clone_lib="NCI_CGAP_Kid14"
FT                   /clone="MGC:18370 IMAGE:4242354"
FT                   /tissue_type="Kidney, normal. 5 month old male mouse."
FT                   /note="Vector: pCMV-SPORT6"
FT                   /db_xref="taxon:10090"
FT   gene            1..2265
FT                   /gene="Slc22a12"
FT                   /note="synonyms: Rst, OAT4L, URAT1"
FT   misc_difference 13
FT                   /gene="Slc22a12"
FT                   /note="'A' in cDNA is 'G' in the mouse genome."
FT   misc_difference 196
FT                   /gene="Slc22a12"
FT                   /note="'G' in cDNA is 'A' in the mouse genome."
FT   CDS             255..1916
FT                   /codon_start=1
FT                   /gene="Slc22a12"
FT                   /product="solute carrier family 22 (organic anion/cation
FT                   transporter), member 12"
FT                   /db_xref="GOA:Q8CFZ5"
FT                   /db_xref="InterPro:IPR005828"
FT                   /db_xref="InterPro:IPR016196"
FT                   /db_xref="InterPro:IPR020846"
FT                   /db_xref="MGI:MGI:1195269"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q8CFZ5"
FT                   /protein_id="AAH35927.1"
FT                   /translation="MAFPELLDRVGGLGRFQLFQTVALVTPILWVTTQNMLENFSAAVP
FT                   HHRCWVPLLDNSTSQASIPGDLGPDVLLAVSIPPGPDQQPHQCLRFRQPQWQLTESNAT
FT                   ATNWSDAATEPCEDGWVYDHSTFRSTIVTTWDLVCNSQALRPMAQSIFLAGILVGAAVC
FT                   GHASDRFGRRRVLTWSYLLVSVSGTAAAFMPTFPLYCLFRFLLASAVAGVMMNTASLLM
FT                   EWTSAQGSPLVMTLNALGFSFGQVLTGSVAYGVRSWRMLQLAVSAPFFLFFVYSWWLPE
FT                   SARWLITVGKLDQGLQELQRVAAVNRRKAEGDTLTMEVLRSAMEEEPSRDKAGASLGTL
FT                   LHTPGLRHRTIISMLCWFAFGFTFYGLALDLQALGSNIFLLQALIGIVDFPVKTGSLLL
FT                   ISRLGRRFCQVSFLVLPGLCILSNILVPHGMGVLRSALAVLGLGCLGGAFTCITIFSSE
FT                   LFPTVIRMTAVGLCQVAARGGAMLGPLVRLLGVYGSWMPLLVYGVVPVLSGLAALLLPE
FT                   TKNLPLPDTIQDIQKQSVKKVTHDTPDGSILMSTRL"
FT   misc_difference 422
FT                   /gene="Slc22a12"
FT                   /note="'C' in cDNA is 'T' in the mouse genome; no amino
FT                   acid change."
FT   misc_difference 626
FT                   /gene="Slc22a12"
FT                   /note="'T' in cDNA is 'C' in the mouse genome; no amino
FT                   acid change."
FT   misc_difference 1121
FT                   /gene="Slc22a12"
FT                   /note="'G' in cDNA is 'A' in the mouse genome; no amino
FT                   acid change."
FT   misc_difference 1473
FT                   /gene="Slc22a12"
FT                   /note="'T' in cDNA is 'C' in the mouse genome; amino acid
FT                   difference: 'F' in cDNA, 'L' in the mouse genome."
FT   misc_difference 2001..2027
FT                   /gene="Slc22a12"
FT                   /note="27 bases in cDNA are not found in the mouse genome."
FT   misc_difference 2063
FT                   /gene="Slc22a12"
FT                   /note="'G' in cDNA is 'T' in the mouse genome."
FT   misc_difference 2066..2101
FT                   /gene="Slc22a12"
FT                   /note="36 bases in cDNA are not found in the mouse genome."
FT   misc_difference 2255..2265
FT                   /gene="Slc22a12"
FT                   /note="polyA tail: 11 bases do not align to the mouse
FT                   genome."
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SQ   Sequence 2265 BP; 491 A; 625 C; 689 G; 460 T; 0 other;
     ctctgctgtg tcactgagag gttcccatgg cctccagatt accacagagg gttccaggtc        60
     tcatggtgac agccagcagg cagatacaca agagccttga ctggctgtga ctcagcaaca       120
     tggctgtgtg acatgcatat gaagagactg gtgtggtcag agccaagggg aaagcaggct       180
     catcacaaag accccgtggt cctgggccag cctctgtgaa gtggaagctg cgtggtggta       240
     cccaggagaa acccatggcc tttcctgaac tcctggaccg agtggggggg ctgggcaggt       300
     tccagctctt ccagacggtg gctctggtga cccccatttt gtgggtcacc acccagaaca       360
     tgctggagaa cttctcagcc gcagttcccc accaccgctg ttgggtgcct ctcctggaca       420
     acagcacctc ccaggcgagt atccctgggg accttggacc cgatgttctt ctggccgtct       480
     ccatcccacc tggtcctgac cagcaacccc accagtgcct ccgcttccga caacctcaat       540
     ggcagcttac agagtccaac gccacggcca ccaactggag tgacgctgcc actgagccat       600
     gcgaggatgg ctgggtttac gaccatagca ccttcaggtc cacaatcgtg accacgtggg       660
     acctggtatg taactcccag gccctgagac ccatggccca gtccatcttc ctggctggaa       720
     tcctggtagg agccgcagtg tgtggccatg cctcagatag gtttgggcgc agaagggtgc       780
     tgacctggag ctatcttctg gtgtccgtgt ccggcacagc agctgccttc atgcccacct       840
     tccccctcta ctgcctgttt cgtttcctgc tggcctctgc agtggcaggt gtcatgatga       900
     acacagccag tctcttgatg gagtggacat cagcccaggg tagccctttg gtgatgacct       960
     tgaacgcctt gggcttcagc tttgggcagg tcctgacagg ttctgtggcc tatggtgtgc      1020
     gcagctggag gatgctacag ctggctgtct ctgccccctt cttcctcttc tttgtttatt      1080
     catggtggct accagaatcg gcacgctggc tcatcacggt gggcaagctg gaccagggcc      1140
     tgcaggaact acagagggta gctgctgtca acaggaggaa ggcagaggga gacacgttga      1200
     ccatggaggt gctgcggtca gctatggagg aggaaccaag cagggacaaa gctggtgcca      1260
     gtctgggcac tctactccac acacctgggc tgcgccaccg aaccatcatc tccatgctgt      1320
     gctggtttgc ctttggcttc accttctacg gcctggccct tgacctgcaa gccctaggaa      1380
     gcaatatctt cctgctccag gcactcatcg ggattgtgga cttcccggtg aagacaggca      1440
     gcctgctgct gatcagccgc ttgggccggc gcttctgcca ggtcagcttc ctggtgctcc      1500
     ccggactctg catcctgtcc aacatactgg tgccccatgg gatgggggtc cttcgctcgg      1560
     ccctggctgt gctggggctg ggctgcctgg ggggcgcctt cacctgcatc accatcttct      1620
     ccagtgagct cttccccact gtgatcagga tgactgcagt gggcctctgc caggtggcag      1680
     cccgaggggg agccatgcta gggcctttgg tacggctgct gggtgtctat ggctcctgga      1740
     tgcccctgct ggtgtatgga gtggtgccag tgttaagtgg cctagctgca ctgctgctgc      1800
     ctgagaccaa gaacttacca ctgcctgaca ccatccaaga catccagaaa cagtcagtga      1860
     agaaggtaac acatgacaca ccagatggct ccatcctgat gtctacccgg ctgtagttgc      1920
     ctggagaagg aacacagcca caataagagg aggaggaaga agaggaggag gaggaagagg      1980
     agaagagaga gagagagaga gagagagaga gagagagaag gaggaggagg aggaggagga      2040
     ggaggaggag gaggaggagg aggaagagga ggaggaggag gaagaggagg aggaggagga      2100
     aagggcaggg ctggcgacgg cgggtaaaga cacctgcctc cgggcctaaa tttctgaaac      2160
     tcatgtgaca acagacttca caaagttgtc ctctgagctc cgcatggaca catacccgcc      2220
     cccggtgata cacacaaata aataattttt aaaaaaaaaa aaaaa                      2265
//


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