Dbfetch
ID BC010362; SV 1; linear; mRNA; STD; HUM; 2698 BP. XX AC BC010362; XX DT 13-JUL-2001 (Rel. 68, Created) DT 15-OCT-2008 (Rel. 97, Last updated, Version 8) XX DE Homo sapiens vacuolar protein sorting 35 homolog (S. cerevisiae), mRNA DE (cDNA clone MGC:13402 IMAGE:4249949), complete cds. XX KW MGC. XX OS Homo sapiens (human) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; OC Homo. XX RN [1] RP 1-2698 RX DOI; 10.1073/pnas.242603899. RX PUBMED; 12477932. RG Mammalian Gene Collection Program Team RA Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D., RA Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F., RA Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., Hopkins R.F., Jordan H., RA Moore T., Max S.I., Wang J., Hsieh F., Diatchenko L., Marusina K., RA Farmer A.A., Rubin G.M., Hong L., Stapleton M., Soares M.B., Bonaldo M.F., RA Casavant T.L., Scheetz T.E., Brownstein M.J., Usdin T.B., Toshiyuki S., RA Carninci P., Prange C., Raha S.S., Loquellano N.A., Peters G.J., RA Abramson R.D., Mullahy S.J., Bosak S.A., McEwan P.J., McKernan K.J., RA Malek J.A., Gunaratne P.H., Richards S., Worley K.C., Hale S., Garcia A.M., RA Gay L.J., Hulyk S.W., Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., RA Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., RA Sanchez A., Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G., RA Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C., Rodriguez A.C., RA Grimwood J., Schmutz J., Myers R.M., Butterfield Y.S., Krzywinski M.I., RA Skalska U., Smailus D.E., Schnerch A., Schein J.E., Jones S.J., Marra M.A.; RT "Generation and initial analysis of more than 15,000 full-length human and RT mouse cDNA sequences"; RL Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002). XX RN [2] RC NIH-MGC Project URL: http://mgc.nci.nih.gov RP 1-2698 RG NIH MGC Project RA ; RT ; RL Submitted (09-JUL-2001) to the INSDC. RL National Institutes of Health, Mammalian Gene Collection (MGC), Bethesda, RL MD 20892-2590, USA XX DR MD5; ee17e11f4905d0b573eeb37e8dd2982d. DR Ensembl-Gn; ENSG00000069329; homo_sapiens. DR Ensembl-Tr; ENST00000299138; homo_sapiens. XX CC Contact: MGC help desk CC Email: cgapbs-r@mail.nih.gov CC Tissue Procurement: CLONTECH CC cDNA Library Preparation: CLONTECH Laboratories, Inc. CC cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) CC DNA Sequencing by: Institute for Systems Biology CC http://www.systemsbiology.org CC contact: amadan@systemsbiology.org CC Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha CC Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting CC Clone distribution: MGC clone distribution information can be found CC through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov CC Series: IRAL Plate: 19 Row: i Column: 6 CC This clone was selected for full length sequencing because it CC passed the following selection criteria: matched mRNA gi: 41352714. CC Differences found between this sequence and the human reference CC genome (build 36) are described in misc_difference features below CC and these differences were also compared to chimpanzee genome CC (build 1). XX FH Key Location/Qualifiers FH FT source 1..2698 FT /organism="Homo sapiens" FT /lab_host="DH10B" FT /mol_type="mRNA" FT /clone_lib="NIH_MGC_83" FT /clone="MGC:13402 IMAGE:4249949" FT /tissue_type="Prostate" FT /note="Vector: pDNR-LIB" FT /db_xref="taxon:9606" FT gene 1..2698 FT /gene="VPS35" FT /note="synonyms: DKFZp434E1211, DKFZp434P1672, MEM3" FT misc_difference 1..3 FT /gene="VPS35" FT /note="3 bases at the 5' end do not align to the human FT genome." FT CDS 21..2411 FT /codon_start=1 FT /gene="VPS35" FT /product="vacuolar protein sorting 35 homolog (S. FT cerevisiae)" FT /db_xref="GOA:Q96QK1" FT /db_xref="H-InvDB:HIT000034898.15" FT /db_xref="HGNC:HGNC:13487" FT /db_xref="InterPro:IPR005378" FT /db_xref="InterPro:IPR016024" FT /db_xref="InterPro:IPR042491" FT /db_xref="PDB:2R17" FT /db_xref="PDB:5F0J" FT /db_xref="PDB:5F0K" FT /db_xref="PDB:5F0L" FT /db_xref="PDB:5F0M" FT /db_xref="PDB:5F0P" FT /db_xref="PDB:5OSH" FT /db_xref="PDB:5OSI" FT /db_xref="UniProtKB/Swiss-Prot:Q96QK1" FT /protein_id="AAH10362.1" FT /translation="MPTTQQSPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKH FT ASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNI FT IPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTRNILPD FT EGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRERERQELRILVGT FT NLVRLSQLEGVNVERYKQIVLTGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNP FT FLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPADIKLFDIFSQQVATVIQSRQ FT DMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELT FT RLLKIPVDTYNNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVFQDQVD FT SIMNLVSTLIQDQPDQPVEDPDPEDFADEQSLVGRFIHLLRSEDPDQQYLILNTARKHF FT GAGGNQRIRFTLPPLVFAAYQLAFRYKENSKVDDKWEKKCQKIFSFAHQTISALIKAEL FT AELPLRLFLQGALAAGEIGFENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTF FT ERMKCFSEENHEPLRTQCALAASKLLKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGK FT RVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKIRED FT LPNLESSEETEQINKHFHNTLEHLRLRRESPESEGPIYEGLIL" FT misc_difference 1378 FT /gene="VPS35" FT /note="'T' in cDNA is 'C' in the human genome; amino acid FT difference: 'F' in cDNA, 'S' in the human genome. The FT chimpanzee genome agrees with the human genomic sequence FT and not the cDNA." FT misc_difference 1958 FT /gene="VPS35" FT /note="'T' in cDNA is 'C' in the human genome; no amino FT acid change. The chimpanzee genome agrees with the human FT genomic sequence and not the cDNA." FT misc_difference 2670..2698 FT /gene="VPS35" FT /note="polyA tail: 29 bases do not align to the human FT genome." XX SQ Sequence 2698 BP; 827 A; 535 C; 584 G; 752 T; 0 other; gggggctctg gggagtcgcc atgcctacaa cacagcagtc ccctcaggat gagcaggaaa 60 agctcttgga tgaagccata caggctgtga aggtccagtc attccaaatg aagagatgcc 120 tggacaaaaa caagcttatg gatgctctaa aacatgcttc taatatgctt ggtgaactcc 180 ggacttctat gttatcacca aagagttact atgaacttta tatggccatt tctgatgaac 240 tgcactactt ggaggtctac ctgacagatg agtttgctaa aggaaggaaa gtggcagatc 300 tctacgaact tgtacagtat gctggaaaca ttatcccaag gctttacctt ttgatcacag 360 ttggagttgt atatgtcaag tcatttcctc agtccaggaa ggatattttg aaagatttgg 420 tagaaatgtg ccgtggtgtg caacatccct tgaggggtct gtttcttcga aattaccttc 480 ttcagtgtac cagaaatatc ttacctgatg aaggagagcc aacagatgaa gaaacaactg 540 gtgacatcag tgattccatg gattttgtac tgctcaactt tgcagaaatg aacaagctct 600 gggtgcgaat gcagcatcag ggacatagcc gagatagaga aaaaagagaa cgagaaagac 660 aagaactgag aattttagtg ggaacaaatt tggtgcgcct cagtcagttg gaaggtgtaa 720 atgtggaacg ttacaaacag attgttttga ctggcatatt ggagcaagtt gtaaactgta 780 gggatgcttt ggctcaagaa tatctcatgg agtgtattat tcaggttttc cctgatgaat 840 ttcacctcca gactttgaat ccttttcttc gggcctgtgc tgagttacac cagaatgtaa 900 atgtgaagaa cataatcatt gctttaattg atagattagc tttatttgct caccgtgaag 960 atggacctgg aatcccagcg gatattaaac tttttgatat attttcacag caggtggcta 1020 cagtgataca gtctagacaa gacatgcctt cagaggatgt tgtatcttta caagtctctc 1080 tgattaatct tgccatgaaa tgttaccctg atcgtgtgga ctatgttgat aaagttctag 1140 aaacaacagt ggagatattc aataagctca accttgaaca tattgctacc agtagtgcag 1200 tttcaaagga actcaccaga cttttgaaaa taccagttga cacttacaac aatattttaa 1260 cagtcttgaa attaaaacat tttcacccac tctttgagta ctttgactac gagtccagaa 1320 agagcatgag ttgttatgtg cttagtaatg ttctggatta taacacagaa attgtctttc 1380 aagaccaggt ggattccata atgaatttgg tatccacgtt gattcaagat cagccagatc 1440 aacctgtaga agaccctgat ccagaagatt ttgctgatga gcagagcctt gtgggccgct 1500 tcattcatct gctgcgctct gaggaccctg accagcagta cttgattttg aacacagcac 1560 gaaaacattt tggagctggt ggaaatcagc ggattcgctt cacactgcca cctttggtat 1620 ttgcagctta ccagctggct tttcgatata aagagaattc taaagtggat gacaaatggg 1680 aaaagaaatg ccagaagatt ttttcatttg cccaccagac tatcagtgct ttgatcaaag 1740 cagagctggc agaattgccc ttaagacttt ttcttcaagg agcactagct gctggggaaa 1800 ttggttttga aaatcatgag acagtcgcat atgaattcat gtcccaggca ttttctctgt 1860 atgaagatga aatcagcgat tccaaagcac agctagctgc catcaccttg atcattggca 1920 cttttgaaag gatgaagtgc ttcagtgaag agaatcatga acctctgagg actcagtgtg 1980 cccttgctgc atccaaactt ctaaagaaac ctgatcaggg ccgagctgtg agcacctgtg 2040 cacatctctt ctggtctggc agaaacacgg acaaaaatgg ggaggagctt cacggaggca 2100 agagggtaat ggagtgccta aaaaaagctc taaaaatagc aaatcagtgc atggacccct 2160 ctctacaagt gcagcttttt atagaaattc tgaacagata tatctatttt tatgaaaagg 2220 aaaatgatgc ggtaacaatt caggttttaa accagcttat ccaaaagatt cgagaagacc 2280 tcccgaatct tgaatccagt gaagaaacag agcagattaa caaacatttt cataacacac 2340 tggagcattt gcgcttgcgg cgggaatcac cagaatccga ggggccaatt tatgaaggtc 2400 tcatccttta aaaaggaaat agctcaccat actcctttcc atgtacatcc agtgagggtt 2460 ttattacgct aggtttccct tccatagatt gtgcctttca gaaatgctga ggtaggtttc 2520 ccatttctta cctgtgatgt gttttaccca gcacctccgg acactcacct tcaggacctt 2580 aataaaatta ttcacttggt aagtgttcaa gtctttctga tcaccccaag tagcatgact 2640 gatctgcaat ttaaaattcc tgtgatctgc aaaaaaaaaa aaaaaaaaaa aaaaaaaa 2698 //