ID AF043342; SV 1; linear; mRNA; STD; HUM; 486 BP.
XX
AC AF043342;
XX
DT 23-FEB-1998 (Rel. 54, Created)
DT 05-JUL-1999 (Rel. 60, Last updated, Version 2)
XX
DE Homo sapiens tumor necrosis factor alpha (TNFa) mRNA, partial cds.
XX
KW .
XX
OS Homo sapiens (human)
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
XX
RN [1]
RP 1-486
RA Jang J.S., Kim B.E.;
RT ;
RL Submitted (15-JAN-1998) to the INSDC.
RL Protein Engineering, General Institute of Technology, Hyundai Pharm. Ind.
RL Co., Ltd., 213 Sosa Bon 1-dong, Sosa-gu, Bucheon 422-231, Korea
XX
DR MD5; d43a1177f724ace1e5358be8c392706e.
DR Ensembl-Gn; ENSG00000204490; homo_sapiens.
DR Ensembl-Gn; ENSG00000206439; homo_sapiens.
DR Ensembl-Gn; ENSG00000223952; homo_sapiens.
DR Ensembl-Gn; ENSG00000228321; homo_sapiens.
DR Ensembl-Gn; ENSG00000228849; homo_sapiens.
DR Ensembl-Gn; ENSG00000230108; homo_sapiens.
DR Ensembl-Gn; ENSG00000232810; homo_sapiens.
DR Ensembl-Tr; ENST00000376122; homo_sapiens.
DR Ensembl-Tr; ENST00000383496; homo_sapiens.
DR Ensembl-Tr; ENST00000412275; homo_sapiens.
DR Ensembl-Tr; ENST00000420425; homo_sapiens.
DR Ensembl-Tr; ENST00000443707; homo_sapiens.
DR Ensembl-Tr; ENST00000448781; homo_sapiens.
DR Ensembl-Tr; ENST00000449264; homo_sapiens.
DR EuropePMC; PMC3993425; 24566630.
DR H-InvDB; HIT000063739_05.4.
DR H-InvDB; HIT000063739_06.4.
DR H-InvDB; HIT000063739_07.4.
DR H-InvDB; HIT000063739_08.4.
DR H-InvDB; HIT000063739_09.4.
DR H-InvDB; HIT000063739_10.4.
DR H-InvDB; HIT000063739_11.4.
XX
CC forward primer (5'-ctcgagtcagatcatcttctcgcaccccg-3')
CC reverse primer (5'-ggaattctgttcgtcctcctcacagggc-3').
XX
FH Key Location/Qualifiers
FH
FT source 1..486
FT /organism="Homo sapiens"
FT /mol_type="mRNA"
FT /cell_type="PHA-treated peripheral blood leukocyte"
FT /db_xref="taxon:9606"
FT gene <1..486
FT /gene="TNFa"
FT CDS <1..474
FT /codon_start=1
FT /gene="TNFa"
FT /product="tumor necrosis factor alpha"
FT /db_xref="GOA:P01375"
FT /db_xref="H-InvDB:HIT000063739_04.4"
FT /db_xref="HGNC:HGNC:11892"
FT /db_xref="InterPro:IPR002959"
FT /db_xref="InterPro:IPR006052"
FT /db_xref="InterPro:IPR006053"
FT /db_xref="InterPro:IPR008983"
FT /db_xref="InterPro:IPR021184"
FT /db_xref="PDB:1A8M"
FT /db_xref="PDB:1TNF"
FT /db_xref="PDB:2AZ5"
FT /db_xref="PDB:2E7A"
FT /db_xref="PDB:2TUN"
FT /db_xref="PDB:2ZJC"
FT /db_xref="PDB:2ZPX"
FT /db_xref="PDB:3ALQ"
FT /db_xref="PDB:3IT8"
FT /db_xref="PDB:3L9J"
FT /db_xref="PDB:3WD5"
FT /db_xref="PDB:4G3Y"
FT /db_xref="PDB:4TSV"
FT /db_xref="PDB:4TWT"
FT /db_xref="PDB:4Y6O"
FT /db_xref="PDB:5M2I"
FT /db_xref="PDB:5M2J"
FT /db_xref="PDB:5M2M"
FT /db_xref="PDB:5MU8"
FT /db_xref="PDB:5TSW"
FT /db_xref="PDB:5UUI"
FT /db_xref="PDB:5WUX"
FT /db_xref="UniProtKB/Swiss-Prot:P01375"
FT /protein_id="AAC03542.1"
FT /translation="VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRD
FT NQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRE
FT TPRGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL"
FT primer_bind <1..24
FT /gene="TNFa"
FT /PCR_conditions="94C-1min, 50C-1min, 72C-3min, 30 cycles;
FT DeltaCycler II from Ericomp"
FT primer_bind complement(466..>486)
FT /gene="TNFa"
XX
SQ Sequence 486 BP; 101 A; 160 C; 133 G; 92 T; 0 other;
gtcagatcat cttctcgcac cccgagtgac aagcctgtag cccatgttgt agcaaaccct 60
caagctgagg ggcagctcca gtggctgaac cgccgggcca atgccctcct ggccaatggc 120
gtggagctga gagataacca gctggtggtg ccatcagagg gcctgtacct catctactcc 180
caggtcctct tcaagggcca aggctgcccc tccacccatg tactcctcac ccacaccatc 240
agccgcatcg ccgtctccta ccagaccaag gtcaacctcc tctctgccat caagagcccc 300
tgccagaggg agaccccgag gggggctgag gccaagccct ggtatgagcc catctatctg 360
ggaggggtct tccagctgga gaagggtgac cgactcagcg ctgagatcaa tcggcccgac 420
tatctcgact ttgccgagtc tgggcaggtc tactttggga tcattgccct gtgaggagga 480
cgaaca 486
//
