Dbfetch
LOCUS NM_165746 3508 bp mRNA linear INV 26-DEC-2023 DEFINITION Drosophila melanogaster phosphofructokinase, transcript variant B (Pfk), mRNA. ACCESSION NM_165746 VERSION NM_165746.3 DBLINK BioProject: PRJNA164 BioSample: SAMN02803731 KEYWORDS RefSeq. SOURCE Drosophila melanogaster (fruit fly) ORGANISM Drosophila melanogaster Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora. REFERENCE 1 (bases 1 to 3508) AUTHORS Matthews,B.B., Dos Santos,G., Crosby,M.A., Emmert,D.B., St Pierre,S.E., Gramates,L.S., Zhou,P., Schroeder,A.J., Falls,K., Strelets,V., Russo,S.M. and Gelbart,W.M. CONSRTM FlyBase Consortium TITLE Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data JOURNAL G3 (Bethesda) 5 (8), 1721-1736 (2015) PUBMED 26109357 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 3508) AUTHORS Crosby,M.A., Gramates,L.S., Dos Santos,G., Matthews,B.B., St Pierre,S.E., Zhou,P., Schroeder,A.J., Falls,K., Emmert,D.B., Russo,S.M. and Gelbart,W.M. CONSRTM FlyBase Consortium TITLE Gene Model Annotations for Drosophila melanogaster: The Rule-Benders JOURNAL G3 (Bethesda) 5 (8), 1737-1749 (2015) PUBMED 26109356 REMARK Publication Status: Online-Only REFERENCE 3 (bases 1 to 3508) AUTHORS Hoskins,R.A., Carlson,J.W., Wan,K.H., Park,S., Mendez,I., Galle,S.E., Booth,B.W., Pfeiffer,B.D., George,R.A., Svirskas,R., Krzywinski,M., Schein,J., Accardo,M.C., Damia,E., Messina,G., Mendez-Lago,M., de Pablos,B., Demakova,O.V., Andreyeva,E.N., Boldyreva,L.V., Marra,M., Carvalho,A.B., Dimitri,P., Villasante,A., Zhimulev,I.F., Rubin,G.M., Karpen,G.H. and Celniker,S.E. TITLE The Release 6 reference sequence of the Drosophila melanogaster genome JOURNAL Genome Res 25 (3), 445-458 (2015) PUBMED 25589440 REFERENCE 4 (bases 1 to 3508) AUTHORS Hoskins,R.A., Carlson,J.W., Kennedy,C., Acevedo,D., Evans-Holm,M., Frise,E., Wan,K.H., Park,S., Mendez-Lago,M., Rossi,F., Villasante,A., Dimitri,P., Karpen,G.H. and Celniker,S.E. TITLE Sequence finishing and mapping of Drosophila melanogaster heterochromatin JOURNAL Science 316 (5831), 1625-1628 (2007) PUBMED 17569867 REFERENCE 5 (bases 1 to 3508) AUTHORS Smith,C.D., Shu,S., Mungall,C.J. and Karpen,G.H. TITLE The Release 5.1 annotation of Drosophila melanogaster heterochromatin JOURNAL Science 316 (5831), 1586-1591 (2007) PUBMED 17569856 REMARK Erratum:[Science. 2007 Sep 7;317(5843):1325] REFERENCE 6 (bases 1 to 3508) AUTHORS Quesneville,H., Bergman,C.M., Andrieu,O., Autard,D., Nouaud,D., Ashburner,M. and Anxolabehere,D. TITLE Combined evidence annotation of transposable elements in genome sequences JOURNAL PLoS Comput Biol 1 (2), 166-175 (2005) PUBMED 16110336 REFERENCE 7 (bases 1 to 3508) AUTHORS Hoskins,R.A., Smith,C.D., Carlson,J.W., Carvalho,A.B., Halpern,A., Kaminker,J.S., Kennedy,C., Mungall,C.J., Sullivan,B.A., Sutton,G.G., Yasuhara,J.C., Wakimoto,B.T., Myers,E.W., Celniker,S.E., Rubin,G.M. and Karpen,G.H. TITLE Heterochromatic sequences in a Drosophila whole-genome shotgun assembly JOURNAL Genome Biol 3 (12), RESEARCH0085 (2002) PUBMED 12537574 REFERENCE 8 (bases 1 to 3508) AUTHORS Kaminker,J.S., Bergman,C.M., Kronmiller,B., Carlson,J., Svirskas,R., Patel,S., Frise,E., Wheeler,D.A., Lewis,S.E., Rubin,G.M., Ashburner,M. and Celniker,S.E. TITLE The transposable elements of the Drosophila melanogaster euchromatin: a genomics perspective JOURNAL Genome Biol 3 (12), RESEARCH0084 (2002) PUBMED 12537573 REFERENCE 9 (bases 1 to 3508) AUTHORS Misra,S., Crosby,M.A., Mungall,C.J., Matthews,B.B., Campbell,K.S., Hradecky,P., Huang,Y., Kaminker,J.S., Millburn,G.H., Prochnik,S.E., Smith,C.D., Tupy,J.L., Whitfied,E.J., Bayraktaroglu,L., Berman,B.P., Bettencourt,B.R., Celniker,S.E., de Grey,A.D., Drysdale,R.A., Harris,N.L., Richter,J., Russo,S., Schroeder,A.J., Shu,S.Q., Stapleton,M., Yamada,C., Ashburner,M., Gelbart,W.M., Rubin,G.M. and Lewis,S.E. TITLE Annotation of the Drosophila melanogaster euchromatic genome: a systematic review JOURNAL Genome Biol 3 (12), RESEARCH0083 (2002) PUBMED 12537572 REFERENCE 10 (bases 1 to 3508) AUTHORS Celniker,S.E., Wheeler,D.A., Kronmiller,B., Carlson,J.W., Halpern,A., Patel,S., Adams,M., Champe,M., Dugan,S.P., Frise,E., Hodgson,A., George,R.A., Hoskins,R.A., Laverty,T., Muzny,D.M., Nelson,C.R., Pacleb,J.M., Park,S., Pfeiffer,B.D., Richards,S., Sodergren,E.J., Svirskas,R., Tabor,P.E., Wan,K., Stapleton,M., Sutton,G.G., Venter,C., Weinstock,G., Scherer,S.E., Myers,E.W., Gibbs,R.A. and Rubin,G.M. TITLE Finishing a whole-genome shotgun: release 3 of the Drosophila melanogaster euchromatic genome sequence JOURNAL Genome Biol 3 (12), RESEARCH0079 (2002) PUBMED 12537568 REFERENCE 11 (bases 1 to 3508) AUTHORS Adams,M.D., Celniker,S.E., Holt,R.A., Evans,C.A., Gocayne,J.D., Amanatides,P.G., Scherer,S.E., Li,P.W., Hoskins,R.A., Galle,R.F., George,R.A., Lewis,S.E., Richards,S., Ashburner,M., Henderson,S.N., Sutton,G.G., Wortman,J.R., Yandell,M.D., Zhang,Q., Chen,L.X., Brandon,R.C., Rogers,Y.H., Blazej,R.G., Champe,M., Pfeiffer,B.D., Wan,K.H., Doyle,C., Baxter,E.G., Helt,G., Nelson,C.R., Gabor,G.L., Abril,J.F., Agbayani,A., An,H.J., Andrews-Pfannkoch,C., Baldwin,D., Ballew,R.M., Basu,A., Baxendale,J., Bayraktaroglu,L., Beasley,E.M., Beeson,K.Y., Benos,P.V., Berman,B.P., Bhandari,D., Bolshakov,S., Borkova,D., Botchan,M.R., Bouck,J., Brokstein,P., Brottier,P., Burtis,K.C., Busam,D.A., Butler,H., Cadieu,E., Center,A., Chandra,I., Cherry,J.M., Cawley,S., Dahlke,C., Davenport,L.B., Davies,P., de Pablos,B., Delcher,A., Deng,Z., Mays,A.D., Dew,I., Dietz,S.M., Dodson,K., Doup,L.E., Downes,M., Dugan-Rocha,S., Dunkov,B.C., Dunn,P., Durbin,K.J., Evangelista,C.C., Ferraz,C., Ferriera,S., Fleischmann,W., Fosler,C., Gabrielian,A.E., Garg,N.S., Gelbart,W.M., Glasser,K., Glodek,A., Gong,F., Gorrell,J.H., Gu,Z., Guan,P., Harris,M., Harris,N.L., Harvey,D., Heiman,T.J., Hernandez,J.R., Houck,J., Hostin,D., Houston,K.A., Howland,T.J., Wei,M.H., Ibegwam,C., Jalali,M., Kalush,F., Karpen,G.H., Ke,Z., Kennison,J.A., Ketchum,K.A., Kimmel,B.E., Kodira,C.D., Kraft,C., Kravitz,S., Kulp,D., Lai,Z., Lasko,P., Lei,Y., Levitsky,A.A., Li,J., Li,Z., Liang,Y., Lin,X., Liu,X., Mattei,B., McIntosh,T.C., McLeod,M.P., McPherson,D., Merkulov,G., Milshina,N.V., Mobarry,C., Morris,J., Moshrefi,A., Mount,S.M., Moy,M., Murphy,B., Murphy,L., Muzny,D.M., Nelson,D.L., Nelson,D.R., Nelson,K.A., Nixon,K., Nusskern,D.R., Pacleb,J.M., Palazzolo,M., Pittman,G.S., Pan,S., Pollard,J., Puri,V., Reese,M.G., Reinert,K., Remington,K., Saunders,R.D., Scheeler,F., Shen,H., Shue,B.C., Siden-Kiamos,I., Simpson,M., Skupski,M.P., Smith,T., Spier,E., Spradling,A.C., Stapleton,M., Strong,R., Sun,E., Svirskas,R., Tector,C., Turner,R., Venter,E., Wang,A.H., Wang,X., Wang,Z.Y., Wassarman,D.A., Weinstock,G.M., Weissenbach,J., Williams,S.M., WoodageT, Worley,K.C., Wu,D., Yang,S., Yao,Q.A., Ye,J., Yeh,R.F., Zaveri,J.S., Zhan,M., Zhang,G., Zhao,Q., Zheng,L., Zheng,X.H., Zhong,F.N., Zhong,W., Zhou,X., Zhu,S., Zhu,X., Smith,H.O., Gibbs,R.A., Myers,E.W., Rubin,G.M. and Venter,J.C. TITLE The genome sequence of Drosophila melanogaster JOURNAL Science 287 (5461), 2185-2195 (2000) PUBMED 10731132 REFERENCE 12 (bases 1 to 3508) AUTHORS Celniker,S., Carlson,J., Wan,K., Pfeiffer,B., Frise,E., George,R., Hoskins,R., Stapleton,M., Pacleb,J., Park,S., Svirskas,R., Smith,E., Yu,C. and Rubin,G. CONSRTM Berkeley Drosophila Genome Project TITLE Drosophila melanogaster release 4 sequence JOURNAL Unpublished REFERENCE 13 (bases 1 to 3508) CONSRTM NCBI Genome Project TITLE Direct Submission JOURNAL Submitted (20-DEC-2023) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA REFERENCE 14 (bases 1 to 3508) CONSRTM FlyBase TITLE Direct Submission JOURNAL Submitted (13-DEC-2023) FlyBase, Harvard University, Biological Laboratories, 16 Divinity Ave, Cambridge, MA 02138, USA REFERENCE 15 (bases 1 to 3508) CONSRTM FlyBase TITLE Direct Submission JOURNAL Submitted (19-OCT-2022) FlyBase, Harvard University, Biological Laboratories, 16 Divinity Ave, Cambridge, MA 02138, USA REFERENCE 16 (bases 1 to 3508) CONSRTM FlyBase TITLE Direct Submission JOURNAL Submitted (20-APR-2020) FlyBase, Harvard University, Biological Laboratories, 16 Divinity Ave, Cambridge, MA 02138, USA REFERENCE 17 (bases 1 to 3508) CONSRTM FlyBase TITLE Direct Submission JOURNAL Submitted (22-APR-2019) FlyBase, Harvard University, Biological Laboratories, 16 Divinity Ave, Cambridge, MA 02138, USA REFERENCE 18 (bases 1 to 3508) CONSRTM FlyBase TITLE Direct Submission JOURNAL Submitted (24-MAY-2018) FlyBase, Harvard University, Biological Laboratories, 16 Divinity Ave, Cambridge, MA 02138, USA REFERENCE 19 (bases 1 to 3508) CONSRTM FlyBase TITLE Direct Submission JOURNAL Submitted (07-DEC-2016) FlyBase, Harvard University, Biological Laboratories, 16 Divinity Ave, Cambridge, MA 02138, USA REFERENCE 20 (bases 1 to 3508) CONSRTM FlyBase TITLE Direct Submission JOURNAL Submitted (07-OCT-2015) FlyBase, Harvard University, Biological Laboratories, 16 Divinity Ave, Cambridge, MA 02138, USA REFERENCE 21 (bases 1 to 3508) AUTHORS Celniker,S., Carlson,J., Kennedy,C., Wan,K., Frise,E., Hoskins,R., Park,S., Svirskas,R. and Karpen,G. TITLE Direct Submission JOURNAL Submitted (10-AUG-2006) Berkeley Drosophila Genome Project, Lawrence Berkeley National Laboratory, One #Cyclotron RoadOne Cyclotron Road, MS 64-121, Berkeley, CA 94720, USA REMARK Direct Submission REFERENCE 22 (bases 1 to 3508) AUTHORS Celniker,S., Carlson,J., Wan,K., Frise,E., Hoskins,R., Park,S., Svirskas,R. and Rubin,G. TITLE Direct Submission JOURNAL Submitted (10-AUG-2006) Berkeley Drosophila Genome Project, Lawrence Berkeley National Laboratory, One Cyclotron Road, MS 64-121, Berkeley, CA 94720, USA REMARK Direct Submission REFERENCE 23 (bases 1 to 3508) AUTHORS Smith,C.D., Shu,S., Mungall,C.J. and Karpen,G.H. CONSRTM Drosophila Heterochromatin Genome Project TITLE Direct Submission JOURNAL Submitted (01-AUG-2006) Drosophila Heterochromatin Genome Project, Ernest Orlando Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Mailstop 64-121, Berkeley, CA 94720, USA REFERENCE 24 (bases 1 to 3508) AUTHORS Adams,M.D., Celniker,S.E., Gibbs,R.A., Rubin,G.M. and Venter,C.J. TITLE Direct Submission JOURNAL Submitted (21-MAR-2000) Celera Genomics, 45 West Gude Drive, Rockville, MD 20850, USA COMMENT REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165746.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.54 URL :: http://flybase.org ##Genome-Annotation-Data-END## FEATURES Location/Qualifiers source 1..3508 /organism="Drosophila melanogaster" /mol_type="mRNA" /db_xref="taxon:7227" /chromosome="2R" /genotype="y[1]; Gr22b[1] Gr22d[1] cn[1] CG33964[R4.2] bw[1] sp[1]; LysC[1] MstProx[1] GstD5[1] Rh6[1]" gene 1..3508 /gene="Pfk" /locus_tag="Dmel_CG4001" /gene_synonym="BcDNA.GH12192; BcDNA:GH12192; CG4001; Dmel\CG4001; l(2)06339; pfk; PFK; PFK1" /note="Phosphofructokinase" /map="46E8-46F1" /db_xref="FLYBASE:FBgn0003071" /db_xref="GeneID:36060" CDS 560..2926 /gene="Pfk" /locus_tag="Dmel_CG4001" /gene_synonym="BcDNA.GH12192; BcDNA:GH12192; CG4001; Dmel\CG4001; l(2)06339; pfk; PFK; PFK1" /EC_number="2.7.1.11" /note="CG4001 gene product from transcript CG4001-RB; CG4001-PB; Pfk-PB; 6-phosphofructokinase; phosphofructokinase 1" /codon_start=1 /product="phosphofructokinase, isoform B" /protein_id="NP_724890.1" /db_xref="FLYBASE:FBpp0087508" /db_xref="GeneID:36060" /db_xref="FLYBASE:FBgn0003071" /translation="MNSEINQRFLARGSQKDKGLAVFTSGGDSQGMNAAVRACVRMAI YLGCKVYFIREGYQGMVDGGDCIQEANWASVSSIIHRGGTIIGSARCQDFRERQGRLK AANNLIQRGITNLVVIGGDGSLTGANLFRQEWSSLLDELVKNKTITTEQQEKFNVLHI VGLVGSIDNDFCGTDMTIGTDTALHRIIEAIDAISSTAYSHQRTFIMEVMGRHCGYLA LVGGLACEADFIFIPEMPPKVDWPDRLCSQLAQERSAGQRLNIVIVAEGAMDREGHPI TAEDVKKVIDERLKHDARITVLGHVQRGGNPSAFDRILACRMGAEATLALMEATKDSV PVVISLDGNQAVRVPLMECVERTQAVAKAMAEKRWADAVKLRGRSFERNLETYKMLTR LKPPKENFDADGKGIEGYRLAVMHIGAPACGMNAAVRSFVRNAIYRGDVVYGINDGVE GLIAGNVRELGWSDVSGWVGQGGAYLGTKRTLPEGKFKEIAARLKEFKIQGLLIIGGF ESYHAAGQIADQRDNYPQFCIPIVVIPSTISNNVPGTEFSLGCDTGLNEITEICDRIR QSAQGTKRRVFVIETMGGYCGYLATLAGLAGGADAAYIYEEKFSIKDLQQDVYHMASK MAEGVSRGLILRNEKASENYSTDFIYRLYSEEGKGLFTCRMNILGHMQQGGSPTPFDR NMGTKMAAKCVDWLAAQIKANIDANGVVNCKSPDTATLLGIVSRQYRFSPLVDLIAET NFDQRIPKKQWWLRLRPLLRILAKHDSAYEEEGMYITVEEECDTDAVA" ORIGIN 1 ttactcgccc agtgataacc agctgatcaa cagctgaacg attgtgggtc gaacataaac 61 aaattggtta aacgctattt tccgctcgtt gctagtcgcg aaaagtccgt tcataacaga 121 cagaatcctc agattttcga cctaaatcaa accaagttag ggcaatcgcg ttggagcgaa 181 ctcttgggcg gatttagcca tccgaagttt gcattgcatc agtacgcact tacagaaagc 241 ccgtggctgt gtgcgtgtgt ccgtgtgtgt tgtgtatgcg tctctggcct gaaggtcacc 301 ttgaaacgtc aaaagcaaaa gcgaaacttg tgaataccgc ctgcgagcgt acatattgct 361 agtaactgaa aagccacggc aaggagcagt agtagtattt atagcccact tggatataac 421 ccagctccag cggcgctgct cgcatagttg caaaacaggg aaactcccta ccgagtacaa 481 tagtggttca tagtgtgtgc tttgtgcgat atccgttacg accccagttt gatttacata 541 tagtgcaaaa gattgaaaaa tgaattccga gattaatcaa cgattcctgg cccgcggctc 601 gcagaaggac aaaggactgg cggtcttcac cagcggcggg gattcccagg gcatgaatgc 661 cgccgtgcga gcctgtgtcc gtatggccat ctatttgggc tgcaaggtct acttcattcg 721 tgaaggctac cagggcatgg tggatggtgg cgactgcatc caggaagcca actgggcctc 781 agtctcatcc attatccatc gtggtggcac catcattggc tccgcccgtt gccaggactt 841 ccgtgagcgt cagggtcgct tgaaagccgc taacaacctg attcagcgag gaatcaccaa 901 tctggtggtc attggaggcg atggctccct cacgggcgcc aatctgttcc gtcaggagtg 961 gtccagcctg ttggacgagc tggtcaagaa caagaccatt accaccgagc agcaggagaa 1021 gttcaatgtc ctgcacatcg ttggattggt tggctccatc gacaacgatt tctgtggcac 1081 tgacatgacc atcggcacgg acacggcact acatcgcatc atcgaggcaa tcgatgccat 1141 ctccagtaca gcctactccc atcagcgcac cttcatcatg gaggtcatgg gtcgtcattg 1201 cggctattta gctcttgtgg gtggactggc gtgcgaggcg gatttcatat tcattcccga 1261 aatgccgccc aaggtcgatt ggccagacag gctctgctct cagctggccc aggagcgatc 1321 tgctggccag cgtctgaaca tcgtgattgt ggccgaggga gccatggatc gcgagggtca 1381 tcccatcaca gccgaggatg taaagaaggt gatcgacgag cgtctgaagc acgatgcccg 1441 catcactgtc ttgggtcacg tgcagcgcgg tggcaatccc agtgccttcg atcgtatttt 1501 ggcttgccgc atgggagctg aggccacttt ggccctgatg gaggccacca aggactcggt 1561 gccagtggtc atctctctgg acggcaacca ggcggttcgc gtgccgctga tggagtgcgt 1621 agagcgcacc caggctgtgg ccaaggccat ggcggagaaa cgctgggcgg atgccgtcaa 1681 gctgcgcgga cgctccttcg agcggaatct ggagacctac aagatgctga cgcgcttgaa 1741 gccgcccaag gagaacttcg acgccgatgg caagggaatc gaaggatacc gcctagctgt 1801 gatgcatatt ggtgctccgg cttgcggtat gaatgccgct gtgcgcagct tcgtgcgcaa 1861 tgccatctac cgcggtgatg tggtttatgg aatcaacgac ggtgtcgagg gtctgattgc 1921 cggaaatgtc cgcgagctgg gctggtcgga tgtctcagga tgggttggtc agggtggcgc 1981 ctacttgggt accaaacgca cactgcctga gggcaagttc aaggagatcg ccgctcgtct 2041 caaggagttt aagatccagg gtctcctgat cattggtggc tttgagagtt accatgccgc 2101 cggacagatc gccgatcagc gggacaacta cccacagttc tgcatcccca ttgtggttat 2161 tccatcgacg atttcgaaca atgtgcccgg cacagaattt tctctgggat gcgacaccgg 2221 tttaaatgag attacggaga tttgcgaccg tatccgtcag tcggcacagg gaaccaagcg 2281 ccgagtgttc gtcattgaga cgatgggtgg ctactgtggc tatttggcca ccttggccgg 2341 cttggccggc ggagctgatg ccgcctacat ctacgaggag aagttctcca tcaaagacct 2401 gcagcaggat gtctaccaca tggcctccaa gatggccgag ggcgtctccc gcggtctaat 2461 cctgcgaaac gagaaggcta gcgagaacta cagcacggac ttcatttacc gcctgtactc 2521 ggaggagggc aagggcctct tcacctgccg tatgaacatc ctgggtcaca tgcagcaggg 2581 cggctcaccc actcccttcg accgcaacat gggcaccaaa atggccgcca agtgtgtcga 2641 ctggctggcc gcccagatca aggcgaacat cgacgccaac ggcgtagtca actgcaagtc 2701 cccggacacc gccacgctgc tgggaatcgt gtcgcggcag taccgcttct caccgctggt 2761 cgacctcatt gcagagacga acttcgatca acgcatcccg aagaagcagt ggtggctgcg 2821 cctgcgtccc ctgctgcgca tcctggccaa gcacgattcc gcctacgagg aggagggtat 2881 gtacatcacc gtcgaggagg agtgtgacac tgacgccgtc gcctaagcgg actcggatcc 2941 ggactctgat ccccacttcc gcttatcacc accatcgctg catcgaagca tcaatgtcca 3001 cctatccgca ccctcgaatc agtttctgtg ttcgcttttt aagttgtttt ggcaccgcat 3061 ccgatccgtt ttatggtttt taccaagatc agacgaagag gcccatgaca gcagcacata 3121 cgaaagtatt tcataatcta cagacgcata aaaaagcaca aatacataac ttatttatat 3181 tgctcgccag tcgattcttt cttatgattt ttccaaatat cgtgtattct tgaattttga 3241 atctcgaata ctgaataacg ttaatagttc attctacatt gcagccgttc cgaagagtct 3301 ggcgacctat tagcggaaaa cgtttcgtat attatcgtaa ttgttggttc ttcagcccaa 3361 ctaaattaaa tgttaaagta tttagacaat ttacccagtc aaaggaacaa attctatctg 3421 caaactgttt ttcgagtatt gttatcgaac gaagcaatga tctatttgta acgacgtgct 3481 aaatacatat tcaaacacct gttggcac //