Dbfetch
LOCUS NM_136455 10364 bp mRNA linear INV 26-DEC-2023 DEFINITION Drosophila melanogaster vacuolar protein sorting 13, transcript variant A (Vps13), mRNA. ACCESSION NM_136455 VERSION NM_136455.3 DBLINK BioProject: PRJNA164 BioSample: SAMN02803731 KEYWORDS RefSeq. SOURCE Drosophila melanogaster (fruit fly) ORGANISM Drosophila melanogaster Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora. REFERENCE 1 (bases 1 to 10364) AUTHORS Matthews,B.B., Dos Santos,G., Crosby,M.A., Emmert,D.B., St Pierre,S.E., Gramates,L.S., Zhou,P., Schroeder,A.J., Falls,K., Strelets,V., Russo,S.M. and Gelbart,W.M. CONSRTM FlyBase Consortium TITLE Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data JOURNAL G3 (Bethesda) 5 (8), 1721-1736 (2015) PUBMED 26109357 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 10364) AUTHORS Crosby,M.A., Gramates,L.S., Dos Santos,G., Matthews,B.B., St Pierre,S.E., Zhou,P., Schroeder,A.J., Falls,K., Emmert,D.B., Russo,S.M. and Gelbart,W.M. CONSRTM FlyBase Consortium TITLE Gene Model Annotations for Drosophila melanogaster: The Rule-Benders JOURNAL G3 (Bethesda) 5 (8), 1737-1749 (2015) PUBMED 26109356 REMARK Publication Status: Online-Only REFERENCE 3 (bases 1 to 10364) AUTHORS Hoskins,R.A., Carlson,J.W., Wan,K.H., Park,S., Mendez,I., Galle,S.E., Booth,B.W., Pfeiffer,B.D., George,R.A., Svirskas,R., Krzywinski,M., Schein,J., Accardo,M.C., Damia,E., Messina,G., Mendez-Lago,M., de Pablos,B., Demakova,O.V., Andreyeva,E.N., Boldyreva,L.V., Marra,M., Carvalho,A.B., Dimitri,P., Villasante,A., Zhimulev,I.F., Rubin,G.M., Karpen,G.H. and Celniker,S.E. TITLE The Release 6 reference sequence of the Drosophila melanogaster genome JOURNAL Genome Res 25 (3), 445-458 (2015) PUBMED 25589440 REFERENCE 4 (bases 1 to 10364) AUTHORS Hoskins,R.A., Carlson,J.W., Kennedy,C., Acevedo,D., Evans-Holm,M., Frise,E., Wan,K.H., Park,S., Mendez-Lago,M., Rossi,F., Villasante,A., Dimitri,P., Karpen,G.H. and Celniker,S.E. TITLE Sequence finishing and mapping of Drosophila melanogaster heterochromatin JOURNAL Science 316 (5831), 1625-1628 (2007) PUBMED 17569867 REFERENCE 5 (bases 1 to 10364) AUTHORS Smith,C.D., Shu,S., Mungall,C.J. and Karpen,G.H. TITLE The Release 5.1 annotation of Drosophila melanogaster heterochromatin JOURNAL Science 316 (5831), 1586-1591 (2007) PUBMED 17569856 REMARK Erratum:[Science. 2007 Sep 7;317(5843):1325] REFERENCE 6 (bases 1 to 10364) AUTHORS Quesneville,H., Bergman,C.M., Andrieu,O., Autard,D., Nouaud,D., Ashburner,M. and Anxolabehere,D. TITLE Combined evidence annotation of transposable elements in genome sequences JOURNAL PLoS Comput Biol 1 (2), 166-175 (2005) PUBMED 16110336 REFERENCE 7 (bases 1 to 10364) AUTHORS Hoskins,R.A., Smith,C.D., Carlson,J.W., Carvalho,A.B., Halpern,A., Kaminker,J.S., Kennedy,C., Mungall,C.J., Sullivan,B.A., Sutton,G.G., Yasuhara,J.C., Wakimoto,B.T., Myers,E.W., Celniker,S.E., Rubin,G.M. and Karpen,G.H. TITLE Heterochromatic sequences in a Drosophila whole-genome shotgun assembly JOURNAL Genome Biol 3 (12), RESEARCH0085 (2002) PUBMED 12537574 REFERENCE 8 (bases 1 to 10364) AUTHORS Kaminker,J.S., Bergman,C.M., Kronmiller,B., Carlson,J., Svirskas,R., Patel,S., Frise,E., Wheeler,D.A., Lewis,S.E., Rubin,G.M., Ashburner,M. and Celniker,S.E. TITLE The transposable elements of the Drosophila melanogaster euchromatin: a genomics perspective JOURNAL Genome Biol 3 (12), RESEARCH0084 (2002) PUBMED 12537573 REFERENCE 9 (bases 1 to 10364) AUTHORS Misra,S., Crosby,M.A., Mungall,C.J., Matthews,B.B., Campbell,K.S., Hradecky,P., Huang,Y., Kaminker,J.S., Millburn,G.H., Prochnik,S.E., Smith,C.D., Tupy,J.L., Whitfied,E.J., Bayraktaroglu,L., Berman,B.P., Bettencourt,B.R., Celniker,S.E., de Grey,A.D., Drysdale,R.A., Harris,N.L., Richter,J., Russo,S., Schroeder,A.J., Shu,S.Q., Stapleton,M., Yamada,C., Ashburner,M., Gelbart,W.M., Rubin,G.M. and Lewis,S.E. TITLE Annotation of the Drosophila melanogaster euchromatic genome: a systematic review JOURNAL Genome Biol 3 (12), RESEARCH0083 (2002) PUBMED 12537572 REFERENCE 10 (bases 1 to 10364) AUTHORS Celniker,S.E., Wheeler,D.A., Kronmiller,B., Carlson,J.W., Halpern,A., Patel,S., Adams,M., Champe,M., Dugan,S.P., Frise,E., Hodgson,A., George,R.A., Hoskins,R.A., Laverty,T., Muzny,D.M., Nelson,C.R., Pacleb,J.M., Park,S., Pfeiffer,B.D., Richards,S., Sodergren,E.J., Svirskas,R., Tabor,P.E., Wan,K., Stapleton,M., Sutton,G.G., Venter,C., Weinstock,G., Scherer,S.E., Myers,E.W., Gibbs,R.A. and Rubin,G.M. TITLE Finishing a whole-genome shotgun: release 3 of the Drosophila melanogaster euchromatic genome sequence JOURNAL Genome Biol 3 (12), RESEARCH0079 (2002) PUBMED 12537568 REFERENCE 11 (bases 1 to 10364) AUTHORS Adams,M.D., Celniker,S.E., Holt,R.A., Evans,C.A., Gocayne,J.D., Amanatides,P.G., Scherer,S.E., Li,P.W., Hoskins,R.A., Galle,R.F., George,R.A., Lewis,S.E., Richards,S., Ashburner,M., Henderson,S.N., Sutton,G.G., Wortman,J.R., Yandell,M.D., Zhang,Q., Chen,L.X., Brandon,R.C., Rogers,Y.H., Blazej,R.G., Champe,M., Pfeiffer,B.D., Wan,K.H., Doyle,C., Baxter,E.G., Helt,G., Nelson,C.R., Gabor,G.L., Abril,J.F., Agbayani,A., An,H.J., Andrews-Pfannkoch,C., Baldwin,D., Ballew,R.M., Basu,A., Baxendale,J., Bayraktaroglu,L., Beasley,E.M., Beeson,K.Y., Benos,P.V., Berman,B.P., Bhandari,D., Bolshakov,S., Borkova,D., Botchan,M.R., Bouck,J., Brokstein,P., Brottier,P., Burtis,K.C., Busam,D.A., Butler,H., Cadieu,E., Center,A., Chandra,I., Cherry,J.M., Cawley,S., Dahlke,C., Davenport,L.B., Davies,P., de Pablos,B., Delcher,A., Deng,Z., Mays,A.D., Dew,I., Dietz,S.M., Dodson,K., Doup,L.E., Downes,M., Dugan-Rocha,S., Dunkov,B.C., Dunn,P., Durbin,K.J., Evangelista,C.C., Ferraz,C., Ferriera,S., Fleischmann,W., Fosler,C., Gabrielian,A.E., Garg,N.S., Gelbart,W.M., Glasser,K., Glodek,A., Gong,F., Gorrell,J.H., Gu,Z., Guan,P., Harris,M., Harris,N.L., Harvey,D., Heiman,T.J., Hernandez,J.R., Houck,J., Hostin,D., Houston,K.A., Howland,T.J., Wei,M.H., Ibegwam,C., Jalali,M., Kalush,F., Karpen,G.H., Ke,Z., Kennison,J.A., Ketchum,K.A., Kimmel,B.E., Kodira,C.D., Kraft,C., Kravitz,S., Kulp,D., Lai,Z., Lasko,P., Lei,Y., Levitsky,A.A., Li,J., Li,Z., Liang,Y., Lin,X., Liu,X., Mattei,B., McIntosh,T.C., McLeod,M.P., McPherson,D., Merkulov,G., Milshina,N.V., Mobarry,C., Morris,J., Moshrefi,A., Mount,S.M., Moy,M., Murphy,B., Murphy,L., Muzny,D.M., Nelson,D.L., Nelson,D.R., Nelson,K.A., Nixon,K., Nusskern,D.R., Pacleb,J.M., Palazzolo,M., Pittman,G.S., Pan,S., Pollard,J., Puri,V., Reese,M.G., Reinert,K., Remington,K., Saunders,R.D., Scheeler,F., Shen,H., Shue,B.C., Siden-Kiamos,I., Simpson,M., Skupski,M.P., Smith,T., Spier,E., Spradling,A.C., Stapleton,M., Strong,R., Sun,E., Svirskas,R., Tector,C., Turner,R., Venter,E., Wang,A.H., Wang,X., Wang,Z.Y., Wassarman,D.A., Weinstock,G.M., Weissenbach,J., Williams,S.M., WoodageT, Worley,K.C., Wu,D., Yang,S., Yao,Q.A., Ye,J., Yeh,R.F., Zaveri,J.S., Zhan,M., Zhang,G., Zhao,Q., Zheng,L., Zheng,X.H., Zhong,F.N., Zhong,W., Zhou,X., Zhu,S., Zhu,X., Smith,H.O., Gibbs,R.A., Myers,E.W., Rubin,G.M. and Venter,J.C. TITLE The genome sequence of Drosophila melanogaster JOURNAL Science 287 (5461), 2185-2195 (2000) PUBMED 10731132 REFERENCE 12 (bases 1 to 10364) AUTHORS Celniker,S., Carlson,J., Wan,K., Pfeiffer,B., Frise,E., George,R., Hoskins,R., Stapleton,M., Pacleb,J., Park,S., Svirskas,R., Smith,E., Yu,C. and Rubin,G. CONSRTM Berkeley Drosophila Genome Project TITLE Drosophila melanogaster release 4 sequence JOURNAL Unpublished REFERENCE 13 (bases 1 to 10364) CONSRTM NCBI Genome Project TITLE Direct Submission JOURNAL Submitted (20-DEC-2023) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA REFERENCE 14 (bases 1 to 10364) CONSRTM FlyBase TITLE Direct Submission JOURNAL Submitted (13-DEC-2023) FlyBase, Harvard University, Biological Laboratories, 16 Divinity Ave, Cambridge, MA 02138, USA REFERENCE 15 (bases 1 to 10364) CONSRTM FlyBase TITLE Direct Submission JOURNAL Submitted (19-OCT-2022) FlyBase, Harvard University, Biological Laboratories, 16 Divinity Ave, Cambridge, MA 02138, USA REFERENCE 16 (bases 1 to 10364) CONSRTM FlyBase TITLE Direct Submission JOURNAL Submitted (20-APR-2020) FlyBase, Harvard University, Biological Laboratories, 16 Divinity Ave, Cambridge, MA 02138, USA REFERENCE 17 (bases 1 to 10364) CONSRTM FlyBase TITLE Direct Submission JOURNAL Submitted (22-APR-2019) FlyBase, Harvard University, Biological Laboratories, 16 Divinity Ave, Cambridge, MA 02138, USA REFERENCE 18 (bases 1 to 10364) CONSRTM FlyBase TITLE Direct Submission JOURNAL Submitted (24-MAY-2018) FlyBase, Harvard University, Biological Laboratories, 16 Divinity Ave, Cambridge, MA 02138, USA REFERENCE 19 (bases 1 to 10364) CONSRTM FlyBase TITLE Direct Submission JOURNAL Submitted (07-DEC-2016) FlyBase, Harvard University, Biological Laboratories, 16 Divinity Ave, Cambridge, MA 02138, USA REFERENCE 20 (bases 1 to 10364) CONSRTM FlyBase TITLE Direct Submission JOURNAL Submitted (07-OCT-2015) FlyBase, Harvard University, Biological Laboratories, 16 Divinity Ave, Cambridge, MA 02138, USA REFERENCE 21 (bases 1 to 10364) AUTHORS Celniker,S., Carlson,J., Kennedy,C., Wan,K., Frise,E., Hoskins,R., Park,S., Svirskas,R. and Karpen,G. TITLE Direct Submission JOURNAL Submitted (10-AUG-2006) Berkeley Drosophila Genome Project, Lawrence Berkeley National Laboratory, One #Cyclotron RoadOne Cyclotron Road, MS 64-121, Berkeley, CA 94720, USA REMARK Direct Submission REFERENCE 22 (bases 1 to 10364) AUTHORS Celniker,S., Carlson,J., Wan,K., Frise,E., Hoskins,R., Park,S., Svirskas,R. and Rubin,G. TITLE Direct Submission JOURNAL Submitted (10-AUG-2006) Berkeley Drosophila Genome Project, Lawrence Berkeley National Laboratory, One Cyclotron Road, MS 64-121, Berkeley, CA 94720, USA REMARK Direct Submission REFERENCE 23 (bases 1 to 10364) AUTHORS Smith,C.D., Shu,S., Mungall,C.J. and Karpen,G.H. CONSRTM Drosophila Heterochromatin Genome Project TITLE Direct Submission JOURNAL Submitted (01-AUG-2006) Drosophila Heterochromatin Genome Project, Ernest Orlando Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Mailstop 64-121, Berkeley, CA 94720, USA REFERENCE 24 (bases 1 to 10364) AUTHORS Adams,M.D., Celniker,S.E., Gibbs,R.A., Rubin,G.M. and Venter,C.J. TITLE Direct Submission JOURNAL Submitted (21-MAR-2000) Celera Genomics, 45 West Gude Drive, Rockville, MD 20850, USA COMMENT REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_136455.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.54 URL :: http://flybase.org ##Genome-Annotation-Data-END## FEATURES Location/Qualifiers source 1..10364 /organism="Drosophila melanogaster" /mol_type="mRNA" /db_xref="taxon:7227" /chromosome="2R" /genotype="y[1]; Gr22b[1] Gr22d[1] cn[1] CG33964[R4.2] bw[1] sp[1]; LysC[1] MstProx[1] GstD5[1] Rh6[1]" gene 1..10364 /gene="Vps13" /locus_tag="Dmel_CG2093" /gene_synonym="BEST:LD02743; CG2093; Dmel\CG2093" /note="Vacuolar protein sorting 13" /map="43D7-43E1" /db_xref="FLYBASE:FBgn0033194" /db_xref="GeneID:35693" CDS 195..10160 /gene="Vps13" /locus_tag="Dmel_CG2093" /gene_synonym="BEST:LD02743; CG2093; Dmel\CG2093" /note="CG2093 gene product from transcript CG2093-RA; CG2093-PA; Vps13-PA" /codon_start=1 /product="vacuolar protein sorting 13, isoform A" /protein_id="NP_610299.2" /db_xref="FLYBASE:FBpp0087984" /db_xref="GeneID:35693" /db_xref="FLYBASE:FBgn0033194" /translation="MVFEAVVADVLNKVLGDYIENLDRNQLKIGIWGGDVVLQNLKIR ENALDELDLPVQLIYGYLGKLVLKIPWKNLYSQPVIVNIEDLYVLVSPNNNVQYNAEK EAKYEMDLKKAALDALEAARKKELEMDQPKADAGFAEKLTAQIVNNLQVQITNVHLRY EDTTTTGSPFSFGISLHELELYTTDCDWEKCYMAQQASQVFKIANLSCLSAYLNCGGQ LYANNKSDLSQQFKTNIACKETKPNYNYVLGPISCNAKLKLNMNPELDDPPFEKPKID LTLEMEKLNVGLTNTQFDNLMKLGDAMNRQQLGIPYRKYRPYNIPYKGHARDWWHFAI TSILEEEVRKPRESWTWGHIKTHRERCNTYAQKYKEQCLSKKPSAVLTETCRLLETEL DVFNLLLIRQRVNIEIAKQREAVPEQKSGWFSGWGWGGGAKKDDQTTSQKLVEKFEAA MTSEEKEKMYRAIGYQENAKPTDLPESYEAIRMNFKLIALEVGLYKDERNSSAATKDF HELPSLVLLNFSMATALITQRPAAEAISIIAGMREIKVTGLTRNDYTPLLVESKITDE FNLLEVFFETNPLDKLCDQRVKVVARPLQITYDAPTILALINAFQTPGDVTLSKFEDA ASTKISNFKERSATGMQYMIDKKAVLDVDILLMPNILVVPHKGVYDAGNVSLLVVSMG QVHLSSQPRRESNKLQHLFSAGEDKDEILKTVMENAYDRFTVAVDDVQMLVVRAGEPW QNALAEANSTEMHVLRPVSLKVTAALCVVDNDPRLPNIKVDIDLPAILVNVSEDRIFL AIKVATSIPLPEQKEPASRLTQTNSRSSMSISNFINKEVKKIGPSASGSSASKDPLLD EIIQYTSLDVNFSLGEINFVLFQSSRKCETSPDVSIEFLTPDGDVLPSQLTENIQEPI EELPPTPPQQILSIDIRRLEAHFVSKTYESVATVKLGDINLRQYDCQDSDMDVLDVIY TPKQENSSNYLFTVSCTIADKSSPEFSTKYNSTEQLVVANFEVLQIVLHQECLQRIME VVNNFQRNLDLVLSSTRPRDRMGSIGGGDGIKRTLNVILEDTEEIMTTDQMKRRKKTR RTHVVETVKVRVIANLDQVGLVLTGRKRPIAEMNVKKFVSSLIIKSSYTEVNIGLKDI QVLDLNPYTIHKNILSIVGKDAFNCQIVIYNKEETQDYNSDDMKITVDIGCMKIIFLN WFVAGVMNFLNNFTAAQATISQAGAAAAESARQKAMDAYETATRMKLNIRIKAPIIIV PIGSQDRNALLLDLGLLELTNNTVEVAVAEEERLAVIDEIKLQICDVKISKIVLLDGN ESTVDEVDAEVGFLSKFNMMNPMSCTLSITRNLSYTWYRDVPELNLSGRLKSIELTLF ADDYALVMLVLNRNLNEGLEEFPPSEEAPQEAQVRPERRNSRAGRLSRTVQVSPIREK IHESIKFNFQFDGVVINLMEGEGAGLARFGIYFLSVKGTKLDNGTLSTSVVLCNIQMD DMRSNSKSQIRQYLSRKDWVQPKLDTDEIIDACYNERNFMVDVTAIIKEDDTFAEVRV RGFDLIVCIDFLLKLTTFLTLPPEENPRESVYIKPAPVSETARDTKHSIRSSAILAAQ ELVPVESSSHEVPNRKMNLILHIDEPDIILVENLEDLNTSCIIFNAQVHLNYRSINDK QIVNGQIDALKMYMCAFLPERREMTRHYILHPCVISLQGSTPEEEGMHISLKLSDIII NVSPATIELLNKAMLSVSSGTMTKCAIAEESRNYSNLWHQHHFHSRTYWFTKVEQGVD ALEAEQRSVSTDNEKQKTEKCVIEIPSITLVIESGVGYYTKPLISLDTRITAVFNNWS RSLTAHGSLTLNMNYYNQALAEWEPIIELNEVIGRNGVREYTPWELKFEMGMEKVQSE LEDDAEQQAMHMNIHSAETLEITLSKTCLGLLSELAEAFSQAIDQNGLTKPDIVAPYV LENDTGFDVNLNLRKGIFTLHEVHRGGTPVGANSTLLMVAQSEEVDPSVIKTCTISTG GRAYLQTKDLSTLSEEDSEDYTLYVTIGDINKEIALPVSKSDTRFFNLMRSTSHEPWG IISEVKQEYGTTKVNIHGVVSVHNHFTTGLNIYRRNPAPTAQCFEDIFVGRVRPGEVF HVPLHAIYAESKDLFFSMRGYRRSVQGISWASNPSDLNYSHQLHCDPTNTFEPLIMNA RRSKSEVYFENTNKYTLLSAFYTIHLRPPLYLRNSLPINIQVSVAGCSVRKEDGLDAQ SSQRFVDRGYRKEDFLDYGEKPVNSGDVLHLPTVRLASKGKESKSFLVVRLVQYLEKD WSCATEIWDYTDDVITWTFSSYDSEMKVDMDLYVKTENRHGSLMLTLFSPFWMINKTG MMLTYKSETTSVEVLYHPPEYSGPILFTFRDKLFFDKKKASIRIDNGQWSEKIPLDVA GSVGEVICFANNQKYPVGVHNHLTQNSLTKQITFIPFYIVCNKCHFDIELQEQSRPAD PWLHLEPNEMEPLWPRNDTKNNLVVRVDGKITPAFDFTEVICTLLKLEDSKYGGINVD VQTTEGGVYITFTDYKPADAPGLLINHTGKQIVYHEKGTKNEHILNAKSTIMYAWDDP TGPKMLVFGTNKEETDLKRDGIGEVIMQDGGKVLWVSFLDGLQRVLLFTENESIANRT ESTASLQSITQSIDLRIHGIGLSVINNETGLDILYLGVTSSGIIWESKKVTKNRFKEL TINENALLEIEYQKYLVHKSVNDVQTYKLDNKFPIDFDLMILKKTVERNLRRSFYPAI WLSRKSSPFQSQLHVKINRIQVDNQFLDPIFPVVLAPIPPPKSVASTTSLKPFIECSM VQRIMPNSTVRQFKYARILIQEFLFKVDLNFLTAIAEMFAKEVSDEAAAKQFRQDVES IELPLSAFFEEHSLEEQKSFYDNLHLGPLKIHVSFSMAGSDTKALPGFLGSLVQGVGV TLTDVNDVVFRLAFFEREYQFFSQKQLINEITSHYTGQALKQLYVLVLGLDVLGNPYG LVVGLKKGVEDLFYEPFQGAIQGPGEFAEGLVLGVKSLFGHTVGGAAGAVSKITGAMG KGLAALTFDEDYQKKRRQGIQNKPKNFHEGLARSSKGLVMGFVDGVTGVVTKPVTGAR DNGVEGFFKGLGKGAIGLVARPTAGVVDFASGSFEAVKRAADASEDVKRMRPPRFQHY DFVLRPYCLMEATGNKIMKETDKGKFATTDNFIHCEEIIQKSEYLVVTNYRVMYVQRN EMFGVWTSLWSYLWNEISSVAATARGVQFTVKTDGKKVLGLFSSKESPRKLVLVADER KRDALVDIIESQRSDPNPLRATIAYPAHN" ORIGIN 1 ttagtttcgg gcgagcattt aaagaacgcg catcgcacgg agttactact tttttgtcgc 61 gtggaaatgc cgctgatcaa ctatagaccg cacagcgaac gccagattgt ttgaagcact 121 caccacgacc acatcaattg gaataacaac cacaaacttg gaggaaaatc aataggagca 181 tctgctagga cacaatggtc ttcgaggcgg tggtggccga tgtgctgaac aaggtgctcg 241 gcgactacat cgagaacttg gaccggaacc agctgaagat tggcatttgg ggaggcgatg 301 tcgtcttgca gaacctcaag atacgggaga atgccctgga cgagttggac ctgcccgtac 361 agcttatcta tggttacttg ggcaaactag tgcttaagat cccctggaag aatttgtaca 421 gccagccggt aattgtcaac attgaggatc tttatgtctt agtttcaccc aacaacaatg 481 tccagtacaa cgccgaaaag gaggccaaat atgagatgga cctcaagaag gcggcgcttg 541 atgccctgga ggcagctcgc aaaaaggagt tggaaatgga tcagcccaaa gcagatgcag 601 gatttgccga aaagctaacg gcgcagatcg ttaacaatct gcaggtgcag ataaccaacg 661 ttcatctgcg atacgaggat accacaacga cgggtagtcc cttctccttc ggcatatccc 721 tgcatgagct cgaactttac accaccgatt gcgactggga gaagtgctac atggcccagc 781 aggcgtcgca ggtcttcaaa atagcgaatc tctcatgtct ttccgcctat ttgaactgtg 841 gtggacaact gtacgcgaac aacaagagcg acctttcaca gcagttcaag acgaatattg 901 cttgcaagga aacaaaaccc aactacaact atgtgctggg tcctatctct tgcaatgcca 961 agctaaagct aaacatgaac ccggagctcg atgatccgcc atttgaaaaa cctaaaatcg 1021 atcttacgct ggaaatggaa aagcttaatg tcggcttgac aaacacacag ttcgacaatc 1081 tcatgaagct tggagacgct atgaaccgcc agcagttggg cataccgtac cggaaatatc 1141 gtccctacaa tataccatac aaaggccacg ccagggactg gtggcacttt gcaataactt 1201 ctattctaga ggaggaagta aggaaaccca gagagagctg gacttgggga cacatcaaga 1261 cccacagaga acgctgcaac acttatgcac agaaatacaa ggagcagtgc ttaagcaaaa 1321 aaccgtcagc cgtgcttacg gaaacctgcc gtttgctaga aacggagctg gacgttttta 1381 acttgctgct aatccggcag agggtcaaca tcgagatagc caagcaacga gaggcagtgc 1441 ctgaacaaaa gtcgggctgg ttcagtggtt ggggatgggg cggcggagcc aagaaggatg 1501 accaaaccac cagtcaaaaa cttgttgaga agtttgaagc ggcaatgacc agtgaagaga 1561 aagagaagat gtacagagct attggttacc aagagaacgc caagcccaca gaccttccag 1621 agagctacga ggccatcagg atgaacttta aactgatagc cttagaggtc ggcctttaca 1681 aggatgagcg taatagttcc gcggcaacga aggacttcca tgagctgccg tcgctggtcc 1741 ttcttaactt ttctatggcc accgcgttga tcacccaacg gccagctgcc gaagccatca 1801 gcattattgc tggcatgaga gaaatcaagg taactggtct cacgcgcaac gattacacgc 1861 ctttattggt tgagtcaaaa atcaccgacg agttcaacct gctggaagtg tttttcgaga 1921 caaacccact ggacaagttg tgcgaccagc gagtgaaggt tgtggcgcgc ccgttgcaga 1981 taacgtatga tgcgccaacc atattggccc tgataaacgc attccagacg cctggcgatg 2041 tcacgctctc caagtttgag gatgctgcca gcacgaaaat ctcgaacttc aaggagcgtt 2101 cagccactgg aatgcagtac atgatcgaca agaaggcagt gctggatgtg gacatcctgt 2161 taatgccaaa catcctggtc gtaccacata aaggagtcta cgatgcgggc aacgtatccc 2221 tgctagtagt tagcatgggc caggtgcacc tctccagcca accgcgtagg gagtcgaaca 2281 agctgcagca tcttttctca gccggcgagg ataaggatga gatcctcaag acggtaatgg 2341 aaaatgctta tgacaggttt acagtcgcag ttgacgatgt gcagatgctg gtcgtgcgag 2401 ctggagagcc gtggcaaaat gcgctagcgg aggcaaactc aacggagatg cacgttttgc 2461 gccccgtctc cctaaaagta actgccgcac tatgtgtggt tgacaatgat ccgcgtcttc 2521 caaatattaa agtggacatc gacttgcctg cgatcctagt taatgtctca gaggatcgca 2581 ttttcctcgc cataaaggtg gcaacaagta ttcccctgcc ggagcaaaag gaaccggcta 2641 gtagactcac acagacgaac tcgcgcagct caatgtccat atccaatttc atcaacaagg 2701 aagtgaaaaa gattgggcct tcggcatcgg ggagctctgc cagtaaggat ccgttgctgg 2761 acgagatcat tcagtacacc agtctcgatg taaatttttc tcttggagaa atcaacttcg 2821 ttttgttcca gtccagtaga aaatgcgaga caagccccga tgtttctatt gaatttctga 2881 ctcctgatgg tgatgtcctt ccttctcagt tgactgagaa tatccaggag cctattgaag 2941 agctaccccc aacgccacct cagcagattc tctcaatcga cattcgacgc ctggaggccc 3001 actttgtgtc caaaacctat gaatcggtgg ccacagtcaa acttggagat atcaacctgc 3061 gacagtatga ctgccaggac agtgacatgg atgtactaga tgttatctat acgccgaaac 3121 aggagaatag ttccaattac ctgttcaccg tctcgtgcac aattgcagac aagtcgtcgc 3181 cagagttcag caccaagtac aactccactg aacagttggt cgtggctaac ttcgaggtac 3241 ttcaaatcgt gctgcaccag gaatgcctac aaaggattat ggaggtcgta aacaatttcc 3301 aaagaaacct agatcttgta ctatcaagta cccgaccacg ggatcggatg ggctccattg 3361 ggggaggaga tggcattaaa cgtacgctta atgttattct tgaggacaca gaggaaataa 3421 tgaccaccga ccagatgaag cggcgaaaga agacgcgccg cactcacgtc gtagagacgg 3481 tgaaagttcg tgttatcgct aatttggatc aagtgggtct ggtgctgacc ggccgaaagc 3541 ggccgatagc agagatgaat gtgaagaaat tcgtgtccag tctgatcata aagtcgtcct 3601 acacggaagt caacattggc cttaaagaca ttcaggtgtt ggacctaaat ccttacacta 3661 ttcacaagaa tattctgagt attgttggca aggacgcttt caactgccag attgtgatct 3721 acaacaagga ggagacccaa gactacaact ctgatgatat gaaaataacc gtggacattg 3781 gctgcatgaa gatcatcttc ctcaattggt ttgtggcagg tgttatgaac ttcttaaaca 3841 acttcacagc ggcccaagca accatatcgc aggctggtgc tgcggctgcc gagtccgctc 3901 gccagaaggc aatggatgcc tatgaaacgg ccactcgtat gaaactaaat atccgcatca 3961 aggctcccat tatcattgtg ccaattgggt cacaggacag gaatgccttg ctgctcgatt 4021 tgggcttact ggagctaacg aataacacgg tggaggtggc ggtggccgaa gaggaacggc 4081 tagctgttat agacgagatc aagttgcaaa tatgcgatgt taagatttcc aaaattgtct 4141 tattagatgg caacgagtca acggttgatg aagtggacgc ggaagtgggt ttcctctcta 4201 aattcaatat gatgaatccc atgagttgca ccttatcaat cacgcgcaat ttgtcataca 4261 catggtaccg ggatgtgccc gaactaaatc tttccggtcg attaaagtcc attgagctca 4321 ctctattcgc ggatgattat gctttggtaa tgcttgttct caatcgcaat ttaaacgagg 4381 gcttggaaga gttccctcca agcgaggagg cgccgcagga agcacaagtt agacccgaaa 4441 gacgcaactc tcgtgcggga cgtttatctc gtaccgttca agtttctccg ataagggaaa 4501 agattcacga atcaatcaag tttaactttc agtttgacgg tgtggtcata aatttaatgg 4561 agggtgaagg agcaggactc gccaggtttg gaatatattt cctctccgtt aagggcacca 4621 agttggacaa cggcacactg agcacttctg ttgtattgtg caatattcaa atggatgaca 4681 tgcgaagtaa ttccaaaagc cagatccgac agtacttgag tcgcaaggat tgggttcagc 4741 ctaagttgga tacagacgag atcatcgatg cctgttacaa cgagcgcaac ttcatggtcg 4801 atgtgacggc tataataaaa gaagatgaca cgttcgctga ggtccgtgtg cgtggcttcg 4861 atctgatcgt ctgcatcgac ttcctgctga aactgactac gtttcttacc ttgccgcctg 4921 aggagaatcc gcgcgagtct gtttatataa agccagcgcc tgtttcggaa actgctcggg 4981 ataccaagca ctctattcgc tcctccgcca tattagccgc ccaagagctg gttcctgtcg 5041 agtcatccag tcacgaagtt ccaaaccgaa aaatgaattt aatactgcat attgacgagc 5101 cagatataat attagtggaa aatctggaag atcttaatac cagttgtatt attttcaatg 5161 ctcaagtgca tttgaattac cgcagcataa acgacaaaca gattgtaaac ggacagatcg 5221 atgctctgaa gatgtacatg tgtgctttcc taccagagcg gcgagagatg acgcgtcatt 5281 atatccttca cccgtgcgtt attagcctgc aaggatcaac tcccgaagaa gagggcatgc 5341 acattagcct aaagctcagt gacattatca tcaacgtgtc gccggccacc atcgagctgc 5401 taaacaaggc aatgctgagc gtctccagtg gcaccatgac caagtgcgct attgccgagg 5461 agtcgcggaa ctattcgaat ctgtggcatc agcaccactt ccatagccgc acgtattggt 5521 tcaccaaggt ggaacagggc gtcgatgccc tggaggctga gcagagaagc gtcagcactg 5581 acaacgaaaa gcagaagaca gaaaagtgtg taattgaaat tcccagcatt acgctcgtga 5641 ttgagtccgg cgtaggctac tacacgaagc cacttatttc gctagacacc cgcattactg 5701 ccgttttcaa caattggagc cgcagtctca ccgctcatgg ctcgttgaca ctaaatatga 5761 attactataa ccaagccctt gccgaatggg agcctattat tgagctgaac gaggtcattg 5821 gtcgcaacgg cgttcgtgaa tacacgccat gggagcttaa gttcgaaatg ggcatggaga 5881 aggtgcagag cgaattggaa gacgatgctg aacagcaggc gatgcacatg aacatccact 5941 cggcagagac gctggagatt acgttgagca agacgtgcct gggtctattg agcgaactgg 6001 ccgaggcctt ctcgcaggct attgaccaga acggtcttac caagccagac atcgtggctc 6061 cttacgtgct ggaaaacgac actggcttcg atgttaacct gaatctgcgg aagggcatct 6121 ttacactgca cgaagtgcac cgcggcggca cacctgtggg tgcaaatagt acccttctta 6181 tggtggccca atctgaagag gtcgatccca gcgtcataaa aacctgcacc atttcgacgg 6241 gcggaagggc atatctgcag actaaggatc tgagcacgtt atcagaagag gattccgagg 6301 actacactct ttatgtgacg attggagata tcaacaagga gatcgcactg cccgtttcca 6361 agtcggacac gcgtttcttc aacctgatgc gcagtacctc tcacgagcct tggggcatta 6421 tttctgaagt aaagcaggag tacggcacaa ccaaagtcaa tatccatggt gttgttagtg 6481 ttcataatca ctttacgacc ggcttgaata tttatcgacg caatcctgcc cctactgcac 6541 aatgtttcga ggatattttc gtcggacgtg tgcgtcccgg cgaagtgttc catgtaccac 6601 tgcacgccat atacgcggaa tccaaggatt tattcttttc catgcgcggc tacagaagat 6661 cagtgcaggg catttcgtgg gcttccaatc cctcggacct taactattcg catcagttgc 6721 attgtgatcc gaccaatacg ttcgaaccgc ttatcatgaa cgctcgaaga tcaaaatcgg 6781 aggtgtactt cgaaaacacc aacaagtata cattgctaag cgccttctac acgattcatc 6841 tgcgccctcc actctatctg cgcaactctc tgcccattaa tatccaagtg tcagttgccg 6901 gctgctctgt ccgaaaagag gatggcctgg atgcacagtc gtcgcagcgc tttgttgaca 6961 gaggctatcg taaggaagac tttctggact atggagaaaa gcctgtaaat tccggcgacg 7021 tgctccacct gccgaccgtt cgattggcct cgaaaggcaa ggaatcgaag agtttcctag 7081 ttgtgcggct ggttcaatat ctggagaaag actggtcctg cgctaccgag atctgggatt 7141 acactgatga tgttattacc tggacgtttt catcatatga ctcggaaatg aaggtggaca 7201 tggatcttta tgtgaaaaca gagaaccgac atggcagctt gatgcttacg ctttttagcc 7261 ctttttggat gatcaataag accggcatga tgctcactta caaatcggaa acgacctccg 7321 tggaagttct ctaccatcct cctgagtatt ctggtcccat tttgttcact ttccgggata 7381 agctattctt tgacaagaaa aaagcttcaa ttcgcataga caatggtcaa tggagtgaaa 7441 agattccatt ggacgtggcg ggttcggttg gcgaagtgat ctgctttgcg aacaatcaga 7501 aataccctgt cggtgttcac aaccacttga cacagaactc tttgaccaag cagataacgt 7561 tcataccctt ttatattgtg tgcaacaagt gtcactttga tatagaactg caggagcaga 7621 gtcgtccagc cgatccatgg ctccatctcg agccaaatga gatggaacct ctctggccga 7681 gaaacgacac aaagaacaat ttggtggtgc gcgtggatgg caagattaca ccggccttcg 7741 actttacgga ggtgatctgt acgctcctga aactggagga cagcaagtac ggcggtatca 7801 atgtggacgt tcagacaacg gaaggcggcg tctatatcac ctttacggac tataagccag 7861 ccgatgcgcc tggcctgctc atcaatcaca ctggcaagca gattgtttac cacgagaagg 7921 gaaccaaaaa cgagcatatc ctaaatgcaa agagtactat aatgtatgcc tgggacgatc 7981 ctacaggtcc aaaaatgctt gtgttcggca caaacaagga agagacggat ctgaaacgag 8041 acggaattgg cgaagtgatc atgcaagacg gcggaaaggt tctatgggta tcattcctcg 8101 acggcctgca acgtgtgctg ctctttacgg aaaacgaatc gatcgccaat cgtacggaaa 8161 gcacggcctc gttacagtct attacccaga gcattgatct gcgtattcac ggcattggct 8221 tgtcagtgat caacaacgag acgggccttg acatacttta tctgggagtt accagctccg 8281 gcattatatg ggagtcaaag aaagtgacca agaatcgctt taaggagtta accattaacg 8341 aaaacgcgct tttggaaatt gagtatcaaa aatatctagt tcacaagagt gtcaacgatg 8401 tgcagaccta caagctagat aacaagttcc cgatcgactt cgaccttatg atcctcaaga 8461 agacggtgga gagaaactta agacgcagtt tctatccagc gatttggttg tcccgcaagt 8521 cgtcgccatt ccaaagccag ctgcatgtga aaattaatcg tatacaggtg gataatcagt 8581 tcctggatcc aatattccct gtggtgttgg cgccaattcc acccccgaag agtgtggcaa 8641 gtaccaccag tcttaaacca tttatcgagt gcagcatggt gcagcgcatc atgccgaact 8701 caactgtgcg tcagttcaag tacgccagga tactgatcca agagttcctc ttcaaagtcg 8761 atttgaactt ccttactgcc atcgccgaaa tgtttgccaa ggaggtctcg gacgaggcag 8821 cagctaagca gttccgtcag gatgttgaat caattgagct cccgctttca gcgttctttg 8881 aagagcactc gttggaggag cagaagagct tttacgataa cctgcatttg ggtccgctca 8941 agatccatgt cagcttctcg atggccgggt cggacacaaa ggcgttgccc ggtttcttgg 9001 gctcattggt ccagggcgtg ggtgtgactc taacggacgt aaacgatgtt gttttccgtc 9061 tggccttctt cgagcgcgag tatcaattct tctcccaaaa gcagctaatc aacgagatca 9121 cctcacacta cactggccaa gcgttaaagc agctgtatgt gctcgtcctc ggacttgatg 9181 tcctgggcaa tccctatggc ctagtggttg gtcttaagaa gggcgtcgaa gacttgttct 9241 atgaaccgtt ccagggtgcc atccaaggtc ctggagaatt cgccgagggt ttggtcctgg 9301 gcgtaaagtc tcttttcggc cacactgtgg gtggtgctgc gggagcggtt tcgaaaataa 9361 ctggtgctat gggaaagggt ttggctgcgc tgaccttcga cgaggactat caaaagaaac 9421 gacgacaggg tattcaaaac aaaccgaaga acttccacga aggattggcc cgcagcagca 9481 agggtctagt gatgggcttt gtggacggtg tcactggcgt tgtaaccaaa ccagtgactg 9541 gggctcgcga taacggggtc gagggcttct tcaagggcct gggtaagggt gcaattggcc 9601 tggttgctcg ccccaccgct ggcgtggttg actttgccag tggcagcttc gaggctgtga 9661 aacgggcggc ggatgcaagc gaggatgtga agcgtatgcg accgccgcgt ttccagcact 9721 acgacttcgt gttgagaccc tattgcttga tggaggcgac agggaataaa ataatgaagg 9781 aaaccgacaa gggtaaattt gccaccacag ataacttcat tcactgtgag gagattatcc 9841 aaaagtccga gtacttggtg gtgaccaatt accgggtgat gtacgtgcaa cgtaacgaga 9901 tgttcggcgt ttggacgtct ctttggtcct acctatggaa cgagattagc tccgtggctg 9961 caacggctcg cggtgtccag ttcacagtaa agacggatgg caagaaggta cttggtctgt 10021 ttagcagcaa ggagtcacct cgaaagttag tgctcgtggc agatgagcgc aaacgtgacg 10081 ctcttgtgga cattatagaa tctcagcgct ccgatcccaa tccgttaagg gccaccatcg 10141 cctatccggc tcataattga caatagaact gaatttattt cccaactata ttataatcca 10201 atcacatccc cacgacagca gcaacgacat ggaaatctac ctcgatcatc aagatttttt 10261 ctgtccaagc accgtgcgcc caattggaaa actcaaactt acatgtcgca cttttacgta 10321 tgttaacaac catggttttg atattaaaat tttatgtcaa agtt //