Dbfetch
LOCUS NM_116971 2746 bp mRNA linear PLN 20-OCT-2022 DEFINITION Arabidopsis thaliana isoamylase 3 (ISA3), mRNA. ACCESSION NM_116971 VERSION NM_116971.6 DBLINK BioProject: PRJNA116 BioSample: SAMN03081427 KEYWORDS RefSeq. SOURCE Arabidopsis thaliana (thale cress) ORGANISM Arabidopsis thaliana Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis. REFERENCE 1 (bases 1 to 2746) AUTHORS Mayer,K., Schuller,C., Wambutt,R., Murphy,G., Volckaert,G., Pohl,T., Dusterhoft,A., Stiekema,W., Entian,K.D., Terryn,N., Harris,B., Ansorge,W., Brandt,P., Grivell,L., Rieger,M., Weichselgartner,M., de Simone,V., Obermaier,B., Mache,R., Muller,M., Kreis,M., Delseny,M., Puigdomenech,P., Watson,M., Schmidtheini,T., Reichert,B., Portatelle,D., Perez-Alonso,M., Boutry,M., Bancroft,I., Vos,P., Hoheisel,J., Zimmermann,W., Wedler,H., Ridley,P., Langham,S.A., McCullagh,B., Bilham,L., Robben,J., Van der Schueren,J., Grymonprez,B., Chuang,Y.J., Vandenbussche,F., Braeken,M., Weltjens,I., Voet,M., Bastiaens,I., Aert,R., Defoor,E., Weitzenegger,T., Bothe,G., Ramsperger,U., Hilbert,H., Braun,M., Holzer,E., Brandt,A., Peters,S., van Staveren,M., Dirske,W., Mooijman,P., Klein Lankhorst,R., Rose,M., Hauf,J., Kotter,P., Berneiser,S., Hempel,S., Feldpausch,M., Lamberth,S., Van den Daele,H., De Keyser,A., Buysshaert,C., Gielen,J., Villarroel,R., De Clercq,R., Van Montagu,M., Rogers,J., Cronin,A., Quail,M., Bray-Allen,S., Clark,L., Doggett,J., Hall,S., Kay,M., Lennard,N., McLay,K., Mayes,R., Pettett,A., Rajandream,M.A., Lyne,M., Benes,V., Rechmann,S., Borkova,D., Blocker,H., Scharfe,M., Grimm,M., Lohnert,T.H., Dose,S., de Haan,M., Maarse,A., Schafer,M., Muller-Auer,S., Gabel,C., Fuchs,M., Fartmann,B., Granderath,K., Dauner,D., Herzl,A., Neumann,S., Argiriou,A., Vitale,D., Liguori,R., Piravandi,E., Massenet,O., Quigley,F., Clabauld,G., Mundlein,A., Felber,R., Schnabl,S., Hiller,R., Schmidt,W., Lecharny,A., Aubourg,S., Chefdor,F., Cooke,R., Berger,C., Montfort,A., Casacuberta,E., Gibbons,T., Weber,N., Vandenbol,M., Bargues,M., Terol,J., Torres,A., Perez-Perez,A., Purnelle,B., Bent,E., Johnson,S., Tacon,D., Jesse,T., Heijnen,L., Schwarz,S., Scholler,P., Heber,S., Francs,P., Bielke,C., Frishman,D., Haase,D., Lemcke,K., Mewes,H.W., Stocker,S., Zaccaria,P., Bevan,M., Wilson,R.K., de la Bastide,M., Habermann,K., Parnell,L., Dedhia,N., Gnoj,L., Schutz,K., Huang,E., Spiegel,L., Sehkon,M., Murray,J., Sheet,P., Cordes,M., Abu-Threideh,J., Stoneking,T., Kalicki,J., Graves,T., Harmon,G., Edwards,J., Latreille,P., Courtney,L., Cloud,J., Abbott,A., Scott,K., Johnson,D., Minx,P., Bentley,D., Fulton,B., Miller,N., Greco,T., Kemp,K., Kramer,J., Fulton,L., Mardis,E., Dante,M., Pepin,K., Hillier,L., Nelson,J., Spieth,J., Ryan,E., Andrews,S., Geisel,C., Layman,D., Du,H., Ali,J., Berghoff,A., Jones,K., Drone,K., Cotton,M., Joshu,C., Antonoiu,B., Zidanic,M., Strong,C., Sun,H., Lamar,B., Yordan,C., Ma,P., Zhong,J., Preston,R., Vil,D., Shekher,M., Matero,A., Shah,R., Swaby,I.K., O'Shaughnessy,A., Rodriguez,M., Hoffmann,J., Till,S., Granat,S., Shohdy,N., Hasegawa,A., Hameed,A., Lodhi,M., Johnson,A., Chen,E., Marra,M., Martienssen,R. and McCombie,W.R. TITLE Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana JOURNAL Nature 402 (6763), 769-777 (1999) PUBMED 10617198 REFERENCE 2 (bases 1 to 2746) CONSRTM NCBI Genome Project TITLE Direct Submission JOURNAL Submitted (19-OCT-2022) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA REFERENCE 3 (bases 1 to 2746) AUTHORS Krishnakumar,V., Cheng,C.-Y., Chan,A.P., Schobel,S., Kim,M., Ferlanti,E.S., Belyaeva,I., Rosen,B.D., Micklem,G., Miller,J.R., Vaughn,M. and Town,C.D. TITLE Direct Submission JOURNAL Submitted (17-MAY-2016) Plant Genomics, J. Craig Venter Institute, 9704 Medical Center Dr, Rockville, MD 20850, USA REMARK Protein update by submitter REFERENCE 4 (bases 1 to 2746) AUTHORS Swarbreck,D., Lamesch,P., Wilks,C. and Huala,E. CONSRTM TAIR TITLE Direct Submission JOURNAL Submitted (18-FEB-2011) Department of Plant Biology, Carnegie Institution, 260 Panama Street, Stanford, CA, USA COMMENT REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_116971.5. FEATURES Location/Qualifiers source 1..2746 /organism="Arabidopsis thaliana" /mol_type="mRNA" /db_xref="taxon:3702" /chromosome="4" /ecotype="Columbia" gene 1..2746 /gene="ISA3" /locus_tag="AT4G09020" /gene_synonym="ATISA3; F23J3.50; F23J3_50; isoamylase 3" /note="Encodes an isoamylase-like protein. Mutant studies show that the gene is strongly involved in starch breakdown. A GUS-protein fusion product was shown to localize to the surface of chloroplastic structures reminiscent of starch granules. In the mutants, the chloroplastic alpha-amylase AMY3 is upregulated." /db_xref="Araport:AT4G09020" /db_xref="GeneID:826481" /db_xref="TAIR:AT4G09020" CDS 191..2485 /gene="ISA3" /locus_tag="AT4G09020" /gene_synonym="ATISA3; F23J3.50; F23J3_50; isoamylase 3" /inference="Similar to RNA sequence, EST:INSD:ES009781.1,INSD:BU636114.1,INSD:ES113153.1, INSD:EL300508.1,INSD:EL973912.1,INSD:ES130732.1, INSD:CB262801.1,INSD:AV809359.1,INSD:ES117010.1, INSD:EH879705.1" /inference="Similar to RNA sequence, mRNA:INSD:BX826408.1" /note="isoamylase 3 (ISA3); FUNCTIONS IN: isoamylase activity, alpha-amylase activity; INVOLVED IN: carbohydrate metabolic process, starch catabolic process; LOCATED IN: chloroplast, chloroplast stroma, chloroplast starch grain; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Glycosyl hydrolase, family 13, all-beta (InterPro:IPR013780), Immunoglobulin-like fold (InterPro:IPR013783), Glycoside hydrolase, family 13, N-terminal (InterPro:IPR004193), Immunoglobulin E-set (InterPro:IPR014756), Glycoside hydrolase, catalytic core (InterPro:IPR017853), Glycoside hydrolase, subgroup, catalytic core (InterPro:IPR013781), Glycosyl hydrolase, family 13, catalytic domain (InterPro:IPR006047); BEST Arabidopsis thaliana protein match is: isoamylase 1 (TAIR:AT2G39930.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink)." /codon_start=1 /product="isoamylase 3" /protein_id="NP_192641.2" /db_xref="GeneID:826481" /db_xref="TAIR:AT4G09020" /db_xref="Araport:AT4G09020" /translation="MLTSPSSSSTYDPFSSNFSPSLTNAFSSSFTIPMGLKLSRRVTR ARIFSRKIKDRSTLKVTCRRAHERVVEEEASTMTETKLFKVSSGEVSPLGVSQVDKGI NFALFSQNATSVTLCLSLSQSGKDDTDDDGMIELVLDPSVNKTGDTWHICVEDLPLNN VLYGYRVDGPGEWQQGHRFDRSILLLDPYAKLVKGHSSFGDSSQKFAQFYGTYDFESS PFDWGDDYKFPNIPEKDLVIYEMNVRAFTADESSGMDPAIGGSYLGFIEKIPHLQDLG INAVELLPVFEFDELELQRRSNPRDHMVNTWGYSTVNFFAPMSRYASGEGDPIKASKE FKEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVYYMLDPNNQLLNFS GCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRATDGSPLSAPPLIRA IAKDSVLSRCKIIAEPWDCGGLYLVGKFPNWDRWAEWNGMYRDDVRRFIKGDSGMKGS FATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGEGGNDGCND NHSWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYGNNNSY GHDTSLNNFQWKELDAKKQNHFRFFSEVIKFRHSHHVLKHENFLTQGEITWHEDNWDN SESKFLAFTLHDGIGGRDIYVAFNAHDYFVKALIPQPPPGKQWFRVADTNLESPDDFV REGVAGVADTYNVAPFSSILLQSK" ORIGIN 1 agaaaacaat gaaagctata cacaaaaact cgcgcttccg ttcacgtggc aatcacttcc 61 aatgaacgat tctccttcgt ttctcccaaa gtcccatcac aatcaaaaac cttctctccg 121 agaatctcac tcactacaaa gagtaagatt cgattcaaaa acaaactccg atcgttgaag 181 caacgaagat atgcttactt caccttcttc ttcttctaca tatgatccat tctcttccaa 241 tttctctccg tcgctcacta atgccttctc tagtagcttc accattccca tgggattgaa 301 attgagtagg agagtcacca gagctcggat cttctctcgt aagattaagg atcgttcaac 361 acttaaggtc acttgtcgtc gcgcacacga acgtgtggtt gaggaagaag catctacaat 421 gacggagacg aaattgttta aagtttcttc tggtgaggta tctcctcttg gtgtgtcaca 481 agtcgataag ggaatcaatt ttgctttgtt ttctcagaat gctacttctg tcacactctg 541 cttatcacta tcccagagtg gtaaggatga tacggatgat gatggtatga tagagttggt 601 tttggatccc agtgtaaaca agactggaga tacttggcac atttgtgttg aggatttgcc 661 tctcaataat gttctctatg gttaccgtgt tgatggtcct ggagaatggc aacaagggca 721 tcggtttgat cgtagtattt tgcttttgga tccctatgct aagctagtta aaggtcatag 781 ttcttttgga gatagtagtc agaagtttgc tcaattttat ggaacttatg actttgagag 841 ctcgccattt gactggggag atgactacaa gtttcctaac atacctgaga aggatcttgt 901 tatttatgaa atgaatgttc gtgcttttac tgctgatgaa tccagtggga tggacccagc 961 tataggaggc agttacctcg gtttcattga gaagatccca caccttcaag atctgggtat 1021 aaatgcagtg gaattattgc cagtgtttga gtttgatgaa ctggagcttc agaggcgttc 1081 taaccctagg gatcacatgg ttaacacatg gggttactcg acagttaact tttttgctcc 1141 gatgagccga tatgctagtg gtgagggaga ccctattaaa gcttcgaaag agtttaaaga 1201 aatggtcaaa gccttgcatt ctgctggtat agaggtcatt ttggacgtag tttataatca 1261 caccaacgaa gcagatgaca aataccctta taccacttca tttcgtggca tagacaacaa 1321 ggtttattac atgcttgatc caaacaacca actgcttaac ttttctggct gtgggaatac 1381 actgaactgt aaccatccag ttgttatgga actcatactt gatagcttaa ggcactgggt 1441 cactgaatat cacgtggatg gtttccggtt tgatcttgct agtgtgcttt gccgagcaac 1501 agatggatct ccactcagtg ctcccccact gataagggca attgccaagg attctgttct 1561 gtcaagatgt aaaataattg cagagccttg ggattgtgga ggattatatc tagtcgggaa 1621 gtttccaaat tgggataggt gggctgagtg gaatgggatg taccgggatg atgttagaag 1681 atttatcaag ggtgacagtg gcatgaaagg aagctttgct actcgggttt ctggatcttc 1741 cgatctttac caggttaacc agaggaagcc ttaccatggt gtaaattttg tgatcgcaca 1801 tgatggattc acattgcgag atcttgtatc gtacaacttt aagcacaatg aggccaatgg 1861 agaaggagga aatgatggat gtaatgacaa tcatagctgg aactgtggtt ttgaagggga 1921 aactggtgat gctcacatca aatctttacg tactcggcaa atgaagaact ttcatttagc 1981 tttgatgatt tctcagggaa caccaatgat gctaatggga gacgaatatg gacatacacg 2041 gtatggtaac aacaatagct acggacatga tacctccctt aacaatttcc agtggaaaga 2101 gctcgacgca aagaagcaga accatttcag gtttttctcg gaggtgatca agttccgaca 2161 ttcacaccat gtactcaagc atgaaaattt cctcacccaa ggtgagatta catggcatga 2221 agataattgg gacaacagtg agagcaagtt cctagctttc actctccatg acggtatagg 2281 tggccgtgac atctatgtgg cattcaacgc ccacgactac tttgtcaagg ctctgattcc 2341 acagccacca ccgggaaaac aatggttccg tgtggctgac acaaacctcg agtcgccgga 2401 tgattttgtg agggaaggtg tggccggtgt ggctgatacc tacaatgtgg ccccattctc 2461 ttcaatcctt ctccagtcca agtaagataa gcactcacac gataacgcac ggtagaggaa 2521 aaataaggtt tatgagttat gagtaaaaaa gatccatgac tccatgagtc tcttgtaaaa 2581 gttgattaca tgtaaaataa taaaatgtac gacatttcca atcacaaata aagatatatt 2641 tagagcaatg gtacattttt ttctaatcat ggaaatacga aatatatgta gtgatattat 2701 taacttaatt ttagttaaat gtattatcta tataatgtaa aagtgg //