Dbfetch
LOCUS NM_001169436 9645 bp mRNA linear INV 26-DEC-2023 DEFINITION Drosophila melanogaster piezo, transcript variant G (Piezo), mRNA. ACCESSION NM_001169436 VERSION NM_001169436.3 DBLINK BioProject: PRJNA164 BioSample: SAMN02803731 KEYWORDS RefSeq. SOURCE Drosophila melanogaster (fruit fly) ORGANISM Drosophila melanogaster Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora. REFERENCE 1 (bases 1 to 9645) AUTHORS Matthews,B.B., Dos Santos,G., Crosby,M.A., Emmert,D.B., St Pierre,S.E., Gramates,L.S., Zhou,P., Schroeder,A.J., Falls,K., Strelets,V., Russo,S.M. and Gelbart,W.M. CONSRTM FlyBase Consortium TITLE Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data JOURNAL G3 (Bethesda) 5 (8), 1721-1736 (2015) PUBMED 26109357 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 9645) AUTHORS Crosby,M.A., Gramates,L.S., Dos Santos,G., Matthews,B.B., St Pierre,S.E., Zhou,P., Schroeder,A.J., Falls,K., Emmert,D.B., Russo,S.M. and Gelbart,W.M. CONSRTM FlyBase Consortium TITLE Gene Model Annotations for Drosophila melanogaster: The Rule-Benders JOURNAL G3 (Bethesda) 5 (8), 1737-1749 (2015) PUBMED 26109356 REMARK Publication Status: Online-Only REFERENCE 3 (bases 1 to 9645) AUTHORS Hoskins,R.A., Carlson,J.W., Wan,K.H., Park,S., Mendez,I., Galle,S.E., Booth,B.W., Pfeiffer,B.D., George,R.A., Svirskas,R., Krzywinski,M., Schein,J., Accardo,M.C., Damia,E., Messina,G., Mendez-Lago,M., de Pablos,B., Demakova,O.V., Andreyeva,E.N., Boldyreva,L.V., Marra,M., Carvalho,A.B., Dimitri,P., Villasante,A., Zhimulev,I.F., Rubin,G.M., Karpen,G.H. and Celniker,S.E. TITLE The Release 6 reference sequence of the Drosophila melanogaster genome JOURNAL Genome Res 25 (3), 445-458 (2015) PUBMED 25589440 REFERENCE 4 (bases 1 to 9645) AUTHORS Hoskins,R.A., Carlson,J.W., Kennedy,C., Acevedo,D., Evans-Holm,M., Frise,E., Wan,K.H., Park,S., Mendez-Lago,M., Rossi,F., Villasante,A., Dimitri,P., Karpen,G.H. and Celniker,S.E. TITLE Sequence finishing and mapping of Drosophila melanogaster heterochromatin JOURNAL Science 316 (5831), 1625-1628 (2007) PUBMED 17569867 REFERENCE 5 (bases 1 to 9645) AUTHORS Smith,C.D., Shu,S., Mungall,C.J. and Karpen,G.H. TITLE The Release 5.1 annotation of Drosophila melanogaster heterochromatin JOURNAL Science 316 (5831), 1586-1591 (2007) PUBMED 17569856 REMARK Erratum:[Science. 2007 Sep 7;317(5843):1325] REFERENCE 6 (bases 1 to 9645) AUTHORS Quesneville,H., Bergman,C.M., Andrieu,O., Autard,D., Nouaud,D., Ashburner,M. and Anxolabehere,D. TITLE Combined evidence annotation of transposable elements in genome sequences JOURNAL PLoS Comput Biol 1 (2), 166-175 (2005) PUBMED 16110336 REFERENCE 7 (bases 1 to 9645) AUTHORS Hoskins,R.A., Smith,C.D., Carlson,J.W., Carvalho,A.B., Halpern,A., Kaminker,J.S., Kennedy,C., Mungall,C.J., Sullivan,B.A., Sutton,G.G., Yasuhara,J.C., Wakimoto,B.T., Myers,E.W., Celniker,S.E., Rubin,G.M. and Karpen,G.H. TITLE Heterochromatic sequences in a Drosophila whole-genome shotgun assembly JOURNAL Genome Biol 3 (12), RESEARCH0085 (2002) PUBMED 12537574 REFERENCE 8 (bases 1 to 9645) AUTHORS Kaminker,J.S., Bergman,C.M., Kronmiller,B., Carlson,J., Svirskas,R., Patel,S., Frise,E., Wheeler,D.A., Lewis,S.E., Rubin,G.M., Ashburner,M. and Celniker,S.E. TITLE The transposable elements of the Drosophila melanogaster euchromatin: a genomics perspective JOURNAL Genome Biol 3 (12), RESEARCH0084 (2002) PUBMED 12537573 REFERENCE 9 (bases 1 to 9645) AUTHORS Misra,S., Crosby,M.A., Mungall,C.J., Matthews,B.B., Campbell,K.S., Hradecky,P., Huang,Y., Kaminker,J.S., Millburn,G.H., Prochnik,S.E., Smith,C.D., Tupy,J.L., Whitfied,E.J., Bayraktaroglu,L., Berman,B.P., Bettencourt,B.R., Celniker,S.E., de Grey,A.D., Drysdale,R.A., Harris,N.L., Richter,J., Russo,S., Schroeder,A.J., Shu,S.Q., Stapleton,M., Yamada,C., Ashburner,M., Gelbart,W.M., Rubin,G.M. and Lewis,S.E. TITLE Annotation of the Drosophila melanogaster euchromatic genome: a systematic review JOURNAL Genome Biol 3 (12), RESEARCH0083 (2002) PUBMED 12537572 REFERENCE 10 (bases 1 to 9645) AUTHORS Celniker,S.E., Wheeler,D.A., Kronmiller,B., Carlson,J.W., Halpern,A., Patel,S., Adams,M., Champe,M., Dugan,S.P., Frise,E., Hodgson,A., George,R.A., Hoskins,R.A., Laverty,T., Muzny,D.M., Nelson,C.R., Pacleb,J.M., Park,S., Pfeiffer,B.D., Richards,S., Sodergren,E.J., Svirskas,R., Tabor,P.E., Wan,K., Stapleton,M., Sutton,G.G., Venter,C., Weinstock,G., Scherer,S.E., Myers,E.W., Gibbs,R.A. and Rubin,G.M. TITLE Finishing a whole-genome shotgun: release 3 of the Drosophila melanogaster euchromatic genome sequence JOURNAL Genome Biol 3 (12), RESEARCH0079 (2002) PUBMED 12537568 REFERENCE 11 (bases 1 to 9645) AUTHORS Adams,M.D., Celniker,S.E., Holt,R.A., Evans,C.A., Gocayne,J.D., Amanatides,P.G., Scherer,S.E., Li,P.W., Hoskins,R.A., Galle,R.F., George,R.A., Lewis,S.E., Richards,S., Ashburner,M., Henderson,S.N., Sutton,G.G., Wortman,J.R., Yandell,M.D., Zhang,Q., Chen,L.X., Brandon,R.C., Rogers,Y.H., Blazej,R.G., Champe,M., Pfeiffer,B.D., Wan,K.H., Doyle,C., Baxter,E.G., Helt,G., Nelson,C.R., Gabor,G.L., Abril,J.F., Agbayani,A., An,H.J., Andrews-Pfannkoch,C., Baldwin,D., Ballew,R.M., Basu,A., Baxendale,J., Bayraktaroglu,L., Beasley,E.M., Beeson,K.Y., Benos,P.V., Berman,B.P., Bhandari,D., Bolshakov,S., Borkova,D., Botchan,M.R., Bouck,J., Brokstein,P., Brottier,P., Burtis,K.C., Busam,D.A., Butler,H., Cadieu,E., Center,A., Chandra,I., Cherry,J.M., Cawley,S., Dahlke,C., Davenport,L.B., Davies,P., de Pablos,B., Delcher,A., Deng,Z., Mays,A.D., Dew,I., Dietz,S.M., Dodson,K., Doup,L.E., Downes,M., Dugan-Rocha,S., Dunkov,B.C., Dunn,P., Durbin,K.J., Evangelista,C.C., Ferraz,C., Ferriera,S., Fleischmann,W., Fosler,C., Gabrielian,A.E., Garg,N.S., Gelbart,W.M., Glasser,K., Glodek,A., Gong,F., Gorrell,J.H., Gu,Z., Guan,P., Harris,M., Harris,N.L., Harvey,D., Heiman,T.J., Hernandez,J.R., Houck,J., Hostin,D., Houston,K.A., Howland,T.J., Wei,M.H., Ibegwam,C., Jalali,M., Kalush,F., Karpen,G.H., Ke,Z., Kennison,J.A., Ketchum,K.A., Kimmel,B.E., Kodira,C.D., Kraft,C., Kravitz,S., Kulp,D., Lai,Z., Lasko,P., Lei,Y., Levitsky,A.A., Li,J., Li,Z., Liang,Y., Lin,X., Liu,X., Mattei,B., McIntosh,T.C., McLeod,M.P., McPherson,D., Merkulov,G., Milshina,N.V., Mobarry,C., Morris,J., Moshrefi,A., Mount,S.M., Moy,M., Murphy,B., Murphy,L., Muzny,D.M., Nelson,D.L., Nelson,D.R., Nelson,K.A., Nixon,K., Nusskern,D.R., Pacleb,J.M., Palazzolo,M., Pittman,G.S., Pan,S., Pollard,J., Puri,V., Reese,M.G., Reinert,K., Remington,K., Saunders,R.D., Scheeler,F., Shen,H., Shue,B.C., Siden-Kiamos,I., Simpson,M., Skupski,M.P., Smith,T., Spier,E., Spradling,A.C., Stapleton,M., Strong,R., Sun,E., Svirskas,R., Tector,C., Turner,R., Venter,E., Wang,A.H., Wang,X., Wang,Z.Y., Wassarman,D.A., Weinstock,G.M., Weissenbach,J., Williams,S.M., WoodageT, Worley,K.C., Wu,D., Yang,S., Yao,Q.A., Ye,J., Yeh,R.F., Zaveri,J.S., Zhan,M., Zhang,G., Zhao,Q., Zheng,L., Zheng,X.H., Zhong,F.N., Zhong,W., Zhou,X., Zhu,S., Zhu,X., Smith,H.O., Gibbs,R.A., Myers,E.W., Rubin,G.M. and Venter,J.C. TITLE The genome sequence of Drosophila melanogaster JOURNAL Science 287 (5461), 2185-2195 (2000) PUBMED 10731132 REFERENCE 12 (bases 1 to 9645) AUTHORS Celniker,S., Carlson,J., Wan,K., Pfeiffer,B., Frise,E., George,R., Hoskins,R., Stapleton,M., Pacleb,J., Park,S., Svirskas,R., Smith,E., Yu,C. and Rubin,G. CONSRTM Berkeley Drosophila Genome Project TITLE Drosophila melanogaster release 4 sequence JOURNAL Unpublished REFERENCE 13 (bases 1 to 9645) CONSRTM NCBI Genome Project TITLE Direct Submission JOURNAL Submitted (20-DEC-2023) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA REFERENCE 14 (bases 1 to 9645) CONSRTM FlyBase TITLE Direct Submission JOURNAL Submitted (13-DEC-2023) FlyBase, Harvard University, Biological Laboratories, 16 Divinity Ave, Cambridge, MA 02138, USA REFERENCE 15 (bases 1 to 9645) CONSRTM FlyBase TITLE Direct Submission JOURNAL Submitted (10-NOV-2022) FlyBase, Harvard University, Biological Laboratories, 16 Divinity Ave, Cambridge, MA 02138, USA REFERENCE 16 (bases 1 to 9645) CONSRTM FlyBase TITLE Direct Submission JOURNAL Submitted (19-OCT-2022) FlyBase, Harvard University, Biological Laboratories, 16 Divinity Ave, Cambridge, MA 02138, USA REFERENCE 17 (bases 1 to 9645) CONSRTM FlyBase TITLE Direct Submission JOURNAL Submitted (20-APR-2020) FlyBase, Harvard University, Biological Laboratories, 16 Divinity Ave, Cambridge, MA 02138, USA REFERENCE 18 (bases 1 to 9645) CONSRTM FlyBase TITLE Direct Submission JOURNAL Submitted (22-APR-2019) FlyBase, Harvard University, Biological Laboratories, 16 Divinity Ave, Cambridge, MA 02138, USA REFERENCE 19 (bases 1 to 9645) CONSRTM FlyBase TITLE Direct Submission JOURNAL Submitted (24-MAY-2018) FlyBase, Harvard University, Biological Laboratories, 16 Divinity Ave, Cambridge, MA 02138, USA REFERENCE 20 (bases 1 to 9645) CONSRTM FlyBase TITLE Direct Submission JOURNAL Submitted (07-DEC-2016) FlyBase, Harvard University, Biological Laboratories, 16 Divinity Ave, Cambridge, MA 02138, USA REFERENCE 21 (bases 1 to 9645) CONSRTM FlyBase TITLE Direct Submission JOURNAL Submitted (07-OCT-2015) FlyBase, Harvard University, Biological Laboratories, 16 Divinity Ave, Cambridge, MA 02138, USA REFERENCE 22 (bases 1 to 9645) AUTHORS Celniker,S., Carlson,J., Kennedy,C., Wan,K., Frise,E., Hoskins,R., Park,S., Svirskas,R. and Karpen,G. TITLE Direct Submission JOURNAL Submitted (10-AUG-2006) Berkeley Drosophila Genome Project, Lawrence Berkeley National Laboratory, One #Cyclotron RoadOne Cyclotron Road, MS 64-121, Berkeley, CA 94720, USA REMARK Direct Submission REFERENCE 23 (bases 1 to 9645) AUTHORS Celniker,S., Carlson,J., Wan,K., Frise,E., Hoskins,R., Park,S., Svirskas,R. and Rubin,G. TITLE Direct Submission JOURNAL Submitted (10-AUG-2006) Berkeley Drosophila Genome Project, Lawrence Berkeley National Laboratory, One Cyclotron Road, MS 64-121, Berkeley, CA 94720, USA REMARK Direct Submission REFERENCE 24 (bases 1 to 9645) AUTHORS Smith,C.D., Shu,S., Mungall,C.J. and Karpen,G.H. CONSRTM Drosophila Heterochromatin Genome Project TITLE Direct Submission JOURNAL Submitted (01-AUG-2006) Drosophila Heterochromatin Genome Project, Ernest Orlando Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Mailstop 64-121, Berkeley, CA 94720, USA REFERENCE 25 (bases 1 to 9645) AUTHORS Adams,M.D., Celniker,S.E., Gibbs,R.A., Rubin,G.M. and Venter,C.J. TITLE Direct Submission JOURNAL Submitted (21-MAR-2000) Celera Genomics, 45 West Gude Drive, Rockville, MD 20850, USA COMMENT REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001169436.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.54 URL :: http://flybase.org ##Genome-Annotation-Data-END## FEATURES Location/Qualifiers source 1..9645 /organism="Drosophila melanogaster" /mol_type="mRNA" /db_xref="taxon:7227" /chromosome="2L" /genotype="y[1]; Gr22b[1] Gr22d[1] cn[1] CG33964[R4.2] bw[1] sp[1]; LysC[1] MstProx[1] GstD5[1] Rh6[1]" gene 1..9645 /gene="Piezo" /locus_tag="Dmel_CG44122" /old_locus_tag="Dmel_CG18103" /old_locus_tag="Dmel_CG8486" /gene_synonym="anon-EST:Liang-1.32; CG18103; CG31608; CG44122; CG8486; clone 1.32; Dmel\CG44122; Dmpiezo; DmPiezo; DmPIEZO; fos28F; fos500; OrfKD; piezo" /map="28E9-28F1" /db_xref="FLYBASE:FBgn0264953" /db_xref="GeneID:34112" CDS 577..8637 /gene="Piezo" /locus_tag="Dmel_CG44122" /old_locus_tag="Dmel_CG18103" /old_locus_tag="Dmel_CG8486" /gene_synonym="anon-EST:Liang-1.32; CG18103; CG31608; CG44122; CG8486; clone 1.32; Dmel\CG44122; Dmpiezo; DmPiezo; DmPIEZO; fos28F; fos500; OrfKD; piezo" /note="CG44122 gene product from transcript CG44122-RG; CG44122-PG; Piezo-PG; dmpiezo; fos-related gene at 28F" /codon_start=1 /product="piezo, isoform G" /protein_id="NP_001162907.1" /db_xref="FLYBASE:FBpp0307170" /db_xref="GeneID:34112" /db_xref="FLYBASE:FBgn0264953" /translation="MVFSYACMVLQRIVVPAVLVLAALMRPVGISFVYLLMFFVSPFV PLATRRNFKGSVTAFFIILLTLSTLVLLGHITLQILAVSLTLPIYNCSFSERLLRHIG FVSFIDLQPFAIIEWLVPEVLVFATSLGSYLTVKRVASQPVGAEQLENGEVVDGQAEN AQTSSQPSAADANGGDVQQATVTTPLQQQQQQLRKRVSMISQHIHFEGLVKISPLFCL ATLFFAAVLRPSVPGGFYFLIFLLSGTYWATCQTLQRGFALLLRCVMVVLVLHSLSIV SYQTPWMQSHLNHTTLTARLIGLEPLIESYCSPDIRVFLYNNKLSLDSYLNPFALFFA YFALALTTKHLIKPRLVRQSTRKARTPQPLESGSSVAPSVTQRGNDMQLESMEQRSEQ ENTTTSILDQISYGFVSVGGFIYQNSYIFTNILMMAWSIVYHSWLTFVLLLSANVLWM IPNQRKAMMRSSPFIVLYAEALLIAQYIYGMDLNNEELPTSVPTAGINLQQIGFERPI ENQMRPCVPLIVKTAFVLMFWVTSRQFFKEKRDRRRDSTLADFIAPLQITVGSAGSSY LINDGKKTSKFLKKAGDVIKNLLVRLWIWLLVLVIFLCAITGENMTGFRICYMALFLF FLLVFQSSSKAWVKIMYGFWLFLIFYAMSILILIYTYQFDKFDTYWSDYLNVSATLQK DIGLKRYQTKDLFLHLVSPTIIVILTVIQVHYFHKRFIASLQQQPLAGGSAQQKPTET TALEPAPSKRRGSAGSLRKSQGPSAEAAPGATTDFETSVRDLVRISFRKIKNKSEYIF KNFKDVFWRFLELHIMKAVYIAAFVCSVSEVCVLHIIFVGFCVLGATSRKAVQVVISR LISFIVTVIVLSKMIYQIEYLSHSQHNVVCSDNRTANNAEWIGLTKADKVTGGLMSLL RTYIIYMVIVTMHAVISLRQLQMRVKIGALNAPPTKLLFPNIIRADAEKDLVGLVKYL LNFGFYKFGIEISLIALVSTITYRQDIVAVVYALWLVVLLLLRRSQCAKIWGVFQAFF AISILTQYIVLVGLPPSSCLVFPWDEGPFGEGIQRWAMLPGALHFNHVPKLIFDFIVL VILNRQKSIFCIEQRYASNDDYPGGSNRSVIADIAQLGRVPFDNPTHDFCSYIRNYSD ILKNGVLCGFYWFTLAVVFLAGTNIADLLALGYLIGAFIFLWQGSDFYLRPIHTIIFR WKWLLAFNVANILIKTSFQMAGCLFMTQLTKDCCWLVHMLGITCTSNVLTEQIMLPEE AELALKPGECPKITHQVVLLWDTICFAFIIFQLRIFKSHYFCHIITDTKANNILASRG ADIIESLRHKQIAHRHDHEKQVLHKIKRKMERIRATQQKMLRPLDKQTHFDEHGYPLP APTVRRRKEIKLHPHATRAGDYYMFEEMDDKFELDLIHDEIDFLEEENITESEMKMQR RKTLYDVWKDLNDAEYRRHFYMRERSYASEPGSRILLKLDDEKEKIGPDLGLASDHDE DDDTIECDLGMRTSTLSEPLDFGRRSQTLLEVKSKDAPTGEFPSTSKGISKERDAATA SSSASPAPTRDVGDLPVIPPPSTGLGREQTSKETSDSKSKMEVDSGEVTAKDSDEDFD TNPIIRLLEGFLVTLTIRLNRFSRNYRFVNRILAGEKKTLKESSSLNRLGLSSAAAMF HFLKSNLESDESEPPASSSTPRRVVIAPPNATEHSDPTSTTLNTNTTTTPLSPPEPLQ PLQPLQPNTTSTPQQQHQHIRAAEEIIELPVDTVDGVAHRKQSINSSPPAKGTMLSRK SDCGLPEIRIKAPSIERGAHYYHNHHSGGGSGSLSKHWSYEQVDSAGEFNLEEENFAQ RDHHIIVEVLISSWYALLANTDLICYIVVFINQVVNASLISLPLPIMVFLWGTLSLPR PTKTFWVTLIAYTQAIVLIKCIFQFKLIWSNYHQLPNQPLTPAKIFGVENKAHYAIYD LILLLVLFLHRYLLKSQGLWKSGYKDTDNQFTKPTASIDERDDSDNLSQPDSRQLNDD AAQKLSLQVSQASLPGSPEFSKTGINQLERTKYTSSLYKFFFSLVHKSRLATDVYALM FLCDFVNFFVLLFGFTAFGTQQTESDEGVQTYLAENKVPIPFLIMLLVQFLLIVIDRA LYLRKALVNKIIFHFFSVIGIHIWMFFVVPAVTERTFNSLAPPIIFYVIKCFYMLLSS YQIKSGYPKRILGNFFTKGFSMVNMIAFKVYMQIPFLYELRTILDWVCIDSTMTIFDW LKMEDIFSNIYLIRCTRQSETDFPAMRAQKKASLSKLIMGGTIVLLIVICIWGPLCLF ALGNAVGTSNVPFHVSLSIRIGPYDPIYTTNNYDSIFEINPEMYSQMTNAYIKEKQAL TFIAGYDATDVAAVRLAGNSPSLWNIAPPDRQRLLNDLRNNHTLKARFSYSLTRKAPA KGLKENVGDEHAISLDESFEGRAALIHMLSETHDVEPIYSNGTTNGTTPEVEEVVVIP GMIPKFIKVLNSGDAAVVSVLSPKHYDYRPLVIKMHRDNETNGLWWEIRDYCNDTFYN ETLSKFAYSNCTSGIVMYTFNDKKFPSTFSFLTAGGIIGLYTTFVLLASRFMKSFIGG QNRKIMFEDLPYVDRVLQLCLDIYLVREALEFALEEDLFAKLLFLYRSPETLIKWTRP KEEYVDDDGDTDSIPSRMSVRRPEQLQPQQPQ" ORIGIN 1 attcagttcg aaatgttttc atctgcgttg gtcgtgcgtc cggtgctccg ctaaaagtaa 61 caaaaagtgc acaaattgcc gggagaaaat gttacattaa cgatattttc ggttaatgct 121 gagcacacgg atacggatac tacgctatat acgcagttcg caaccaaaat cgatgcaata 181 cgcggatttg ggcaaacaca acgcagtgcc tgtaaaaatc gaagccgaaa cgaaacaata 241 acaaatgcgc taaagttctt accaaataat ttgcaccgtc agaaaaagag tggtgaaaag 301 tggtcaaaag tgatcgaaaa gatctgaaaa gttcgttaaa ataaagaaag tgtatggtta 361 agtgtgcgtt cctggtgata aatcggtaaa aatcgttcgc tttcgttttt gttgtggtcg 421 ccgttcgcag agcttatgca aatgtgctgc tctgcgccgc ttgtgacaac aaattgcact 481 cgaagctgag gcttttatga atgctactgc gttggagtcg gagtctgagt ccggatacgc 541 atctggagga gaacggcgcc ggaggcatcc atcaaaatgg tcttcagcta tgcgtgcatg 601 gtgctccagc gcatcgtggt gccagcggtt ctggtactcg ctgccctgat gcgaccagtg 661 ggcatatcct ttgtctacct tttgatgttc tttgtgtcgc cctttgttcc gctggccacg 721 cgacgcaact tcaaaggatc tgtgactgcc ttcttcatca tcctgctgac gttgagcacg 781 ctggtcctct tgggtcacat aacgctacag attctggcgg tcagcctcac gctgcccatc 841 tacaattgct cgttcagtga gcgtctgctg cgtcacattg gcttcgtgag ctttattgat 901 ctacagccat ttgccatcat cgaatggcta gtgcctgagg tgttggtttt cgccacctcc 961 ctgggttcgt atctcacggt gaagcgagtg gcctcacagc ccgtcggcgc cgagcagctg 1021 gagaacggcg aggtggtcga tggccaggcg gaaaacgcgc agacatcttc tcagccatct 1081 gccgcagatg ccaatggagg agatgtgcaa caggccacgg tcaccacgcc gctgcagcaa 1141 cagcagcagc agctgaggaa acgagtgtcc atgatcagcc agcacataca ctttgagggc 1201 ttggtcaaga tctctcctct gttctgcctg gccacgctgt tctttgcggc cgtactgcgt 1261 ccctcggtgc ctggcggatt ttactttctc attttcctgc tgtccggcac ctactgggca 1321 acatgccaga cgctgcaacg gggcttcgct ttgttgctgc gctgcgtgat ggtcgtcctc 1381 gtgctgcact ccctgtccat tgtgtcatac cagacgccat ggatgcagag ccacctcaat 1441 cataccaccc tgacagctcg attgattgga ctggaaccgc ttattgaatc ctactgctcg 1501 ccggatatac gagtctttct gtacaataat aagctatccc tggactcgta cctcaatccc 1561 tttgcgttgt tctttgccta tttcgcactg gcactgacca ccaagcatct gattaagcca 1621 cggttggtgc ggcaaagcac gcgaaaggca cgtacacccc aaccgctgga aagtggatcc 1681 tcggtggcgc caagtgtcac tcagcggggc aacgacatgc agctggaatc gatggagcag 1741 cgatcggaac aggagaacac caccacatcc atactggatc aaatatcgta tggattcgtc 1801 agcgtgggag gattcatata tcagaatagc tatatattca ccaatattct tatgatggcc 1861 tggtccatag tgtatcacag ttggctgact tttgtcctct tgctgtcggc caacgtactg 1921 tggatgattc ccaaccagag gaaggccatg atgcggtcca gtccctttat agttctgtat 1981 gctgaggccc ttttgattgc ccaatacata tacggcatgg atctcaacaa cgaagagctg 2041 cccacgagcg ttcccactgc gggcattaac ctgcaacaaa ttggcttcga acgacccatc 2101 gagaaccaaa tgcggccatg tgtgccgctg atcgtgaaga cagcgtttgt gctaatgttt 2161 tgggtgacat cacgacagtt cttcaaggag aagcgcgatc gccgacggga cagcactctg 2221 gcggacttca ttgccccact gcagattact gtgggatcgg ctggctccag ctacctcatc 2281 aacgatggca agaagacctc aaagttccta aagaaggccg gcgatgtgat caaaaatcta 2341 ttggtgcgtc tgtggatctg gctactcgtg ctggttatct tcctttgcgc catcactggc 2401 gagaacatga ccggcttccg catctgctac atggccctgt ttctattctt cttgctagtc 2461 tttcaatctt cgtccaaggc gtgggttaag atcatgtacg gcttctggct gtttctgatc 2521 ttctatgcca tgtccatact tatattgatc tacacatatc aattcgacaa gttcgacacg 2581 tactggagcg actatcttaa tgtgtccgcg actttgcaaa aggacatcgg ccttaagcgc 2641 tatcagacca aggatctgtt cctccatttg gtctcaccga cgataattgt gatcctgacc 2701 gtcatacaag tgcactactt ccacaagcgc tttattgcat cattgcagca gcagccgttg 2761 gctggcggat cggcacagca gaaacccacg gagacaactg ccttggaacc ggcgccatcg 2821 aagcgacgtg gcagcgccgg ttcactgcgt aaatctcagg gtccatcggc ggaggctgct 2881 ccaggagcca ccaccgattt cgagacatct gtgcgagact tggtgcgcat atcgttccgc 2941 aagatcaaga acaagtcgga gtacatcttc aagaacttca aggatgtctt ctggcgcttc 3001 ctggagctgc acatcatgaa ggctgtgtat atcgcagcct tcgtgtgcag tgtcagcgaa 3061 gtctgcgtac tgcacattat ctttgtgggt ttctgtgtgc tgggcgccac ctcgcggaag 3121 gccgttcagg tggtgatcag ccgcctcatc tcgttcattg tcaccgtcat agttctgtcc 3181 aagatgatct accagatcga gtacttgagt cactcgcagc acaatgtggt ttgttctgac 3241 aaccggactg ccaacaatgc ggagtggatt ggtctcacca aggctgacaa ggtaactggc 3301 ggactgatga gcctgttgcg cacctacatc atctacatgg ttattgtgac catgcacgca 3361 gtgatcagtt tgcggcagct tcaaatgcgc gtcaagatcg gagcactgaa tgctccaccc 3421 accaagctgc tgttccccaa tattattcga gctgatgctg agaaggatct ggtgggactg 3481 gtcaagtatc tcctcaactt tggcttctac aaattcggca ttgagatatc gctaatcgcg 3541 ctggtctcta ccatcacata tcgccaggat attgttgccg tagtctatgc tctgtggcta 3601 gtggtgcttt tgcttctacg gagatcgcag tgcgccaaaa tatggggcgt gtttcaggca 3661 ttctttgcca tctccatact gacacagtat atagtgctgg taggattgcc gccgagctca 3721 tgcctggtgt ttccctggga tgaaggtccc ttcggcgagg gcatacaacg ctgggcgatg 3781 ctgccaggag ccctgcactt caaccacgta cccaagctga tcttcgactt tattgtcttg 3841 gtcattctga atcgacagaa gagtatcttc tgcatcgaac agcgttatgc cagtaacgat 3901 gactacccgg gtggcagcaa tcgcagtgtg atcgcggata ttgctcagct aggtcgcgtt 3961 cccttcgaca atcccaccca cgacttttgt tcgtacatac gcaactactc ggacatcctt 4021 aagaacggag tgctgtgcgg cttctactgg tttactctgg cagttgtttt cttggccggc 4081 accaatattg ctgatctgct ggcactgggt tatctgatcg gagcgtttat cttcctgtgg 4141 cagggatcgg atttctatct gcgtcccata cacaccatca tctttcgctg gaagtggctg 4201 ctggcattca atgtggccaa catacttatc aagacgtcct tccaaatggc cggctgtttg 4261 ttcatgaccc aactgacgaa agactgctgc tggctggtgc acatgctcgg catcacatgt 4321 acgagcaatg tacttacaga gcaaataatg ctgcccgagg aggctgaatt ggcgcttaag 4381 ccaggcgaat gtcctaagat cacccaccag gtggtccttc tgtgggacac gatttgcttt 4441 gccttcatca tcttccagct gcgcatcttc aagtcgcact acttctgtca catcataacg 4501 gacaccaaag caaataacat cctggcctca agaggagccg acatcattga gagcctacga 4561 cataagcaga ttgcacatcg acacgaccat gaaaagcagg tgctgcataa gatcaagcga 4621 aagatggagc gcatccgtgc cacgcagcag aagatgctac gacccttgga caaacaaacc 4681 cacttcgacg aacatggtta tccacttcct gcaccaacag tacgcagaag gaaggaaatt 4741 aaattacatc cacatgctac ccgtgctggt gactactata tgttcgagga gatggacgat 4801 aagtttgagc tcgacttgat acacgacgag atcgacttcc tcgaggagga gaacatcacc 4861 gagagcgaga tgaagatgca gcgacgcaag accctctacg atgtgtggaa ggatctgaat 4921 gatgccgagt atcgtcgcca cttctatatg cgtgagcgtt cctacgcctc agagcccggc 4981 tcacgcattc tcctgaagtt ggacgacgag aaggagaaga ttgggcccga tctcggcctg 5041 gccagcgacc acgatgagga cgacgatacc attgagtgcg accttggcat gcgcacctcg 5101 acgctgtccg aaccgctcga ctttggtcgt cgcagccaga cgctgctcga ggttaagtcg 5161 aaggatgcac ccacaggcga gtttccctcc accagtaagg gtatttcgaa ggagcgcgat 5221 gcggcaacgg cttccagttc ggctagtcca gcgcccacta gggatgtggg tgatctgcct 5281 gtgattccac caccctccac tggcctggga cgtgagcaaa cctccaagga gacctccgat 5341 agcaagtcca aaatggaagt ggacagcgga gaggtgacgg ccaaggattc ggatgaggac 5401 ttcgatacca atcccattat cagactgctc gagggcttct tggtcacgct gaccataaga 5461 ctgaaccgct tttcgcgcaa ctatcgcttt gtgaatcgca tcctggccgg cgaaaagaag 5521 accctaaagg agtccagttc gttgaaccgt ctggggctgt ccagtgccgc tgccatgttc 5581 cacttcctca aatcgaattt agagagcgat gaaagtgaac cgcccgcctc ctcatccacc 5641 ccgcggcggg tggtgatcgc accaccgaat gccaccgagc actcagaccc caccagcacc 5701 acactgaaca cgaacacgac aaccacaccg ctatcaccac cagaaccact gcaaccactg 5761 caaccactgc aaccaaatac aaccagtaca ccacagcaac agcatcagca tattcgcgct 5821 gccgaagaaa tcatcgaact gcctgtagat accgtggatg gagtcgccca tagaaaacaa 5881 tcaatcaatt catcgccgcc agccaagggc acgatgctca gtcgaaaatc ggattgtggc 5941 ctgcctgaga tccgtattaa agcgccatct atcgagcgcg gtgcacacta ttaccataat 6001 caccacagcg gcggtggctc aggatccttg agcaaacact ggtcctacga gcaggtggac 6061 agcgcgggtg aattcaatct ggaggaggag aacttcgccc agagagatca tcacatcatt 6121 gtcgaggtgt tgatctcctc gtggtatgcc ctgttggcca acacggatct catctgctac 6181 attgtggttt tcatcaatca ggtggtcaat gccagtctca tttcgctgcc gctgcccatt 6241 atggtctttt tgtggggcac attgtctctg ccgcgtccca ctaaaacctt ctgggtcacg 6301 ctgattgcct acacccaggc catcgtgctg atcaagtgca tcttccagtt taagctgatc 6361 tggtccaatt accaccaact gcccaatcag ccgctaacac cggccaaaat attcggcgtt 6421 gagaataagg cccattatgc gatttacgac ctgatactat tgctggttct attcctgcat 6481 cgctatttgc ttaaatcaca aggcctgtgg aaatcgggct acaaggacac ggataaccag 6541 ttcaccaagc ccaccgctag cattgatgaa cgcgacgata gcgacaactt atcccaaccg 6601 gattcccgcc agctaaacga tgatgctgct cagaagctga gtctccaagt gagccaggct 6661 tccttgccag gatcgcccga gtttagcaag acaggcatca atcagctaga gcgcaccaag 6721 tacacttcat cgctgtacaa attcttcttc agtttggtcc acaaatcccg tctagccaca 6781 gatgtttacg ctctgatgtt cctctgcgat tttgtgaact tcttcgtgct tcttttcggc 6841 ttcactgctt ttggaactca gcaaacggaa agtgatgaag gtgtgcagac atatcttgcg 6901 gagaacaaag tgcccatacc attcctgatt atgctacttg ttcagttcct gctcattgtc 6961 attgatcgag ctttgtatct gcgcaaagcc ctggtgaaca agatcatctt ccacttcttt 7021 tcggttatcg gaatacacat ctggatgttc ttcgttgtgc ctgcagttac ggagcgcact 7081 ttcaactccc tcgcacctcc aataatattc tacgttatca agtgctttta catgctgctc 7141 agctcttatc aaatcaaatc cggttaccct aagcgcattc taggcaactt cttcaccaag 7201 ggtttctcga tggtcaatat gattgccttt aaggtgtaca tgcagattcc attcctgtac 7261 gagctgcgaa ccatattaga ttgggtgtgc attgacagca ccatgaccat tttcgattgg 7321 ctaaagatgg aggacatttt ctcgaatata tatcttatcc gctgcacccg gcagtcagag 7381 actgattttc cggccatgag agctcagaag aaggcctcac tttcaaagct gataatgggt 7441 ggcaccatcg tcctgctgat agtgatttgc atttggggac cattgtgttt gtttgcactt 7501 ggcaacgcgg tgggcacctc gaatgtgccg ttccatgtgt cgctatcaat acgaattgga 7561 ccctatgatc ccatctacac aacgaacaac tacgacagta tcttcgagat caatcctgag 7621 atgtactctc agatgactaa tgcatacata aaggagaaac aggctttgac gtttattgcc 7681 ggctatgatg caacggatgt agcggcggtt agactagctg gcaactcgcc atctctgtgg 7741 aacatagcac cgccggatag gcagcgacta ctaaatgatt taagaaacaa tcacacgctg 7801 aaggcccggt tctcttattc cctcacccga aaggctcctg ccaaggggct aaaggaaaat 7861 gtgggcgacg agcatgccat atcgctggac gagtcctttg agggacgagc agcactcatt 7921 cacatgctaa gcgaaaccca tgatgtggag cccatttaca gcaatggcac cacaaatgga 7981 accactcccg aagttgaaga agtggtggtg atacccggaa tgatacccaa gtttatcaag 8041 gttctcaatt cgggcgacgc tgcggtggta agtgttttga gcccaaaaca ctacgactac 8101 cgaccgttgg ttatcaaaat gcatcgggac aacgagacca atgggttgtg gtgggagatt 8161 cgggactatt gcaacgacac cttctacaac gaaactctat cgaagtttgc atacagcaac 8221 tgcacttcgg gaattgtgat gtacacgttc aacgataaaa agtttccatc cacgttcagt 8281 ttcctcacag cgggaggcat cattggtctg tacactacct ttgtgttatt ggcctcgcgc 8341 tttatgaagt ccttcattgg cgggcaaaac agaaagatca tgtttgagga tctgccctat 8401 gttgatagag tgctgcagct ctgtctggac atttatctgg tacgggaggc attggaattc 8461 gctttggaag aggacctgtt tgccaaatta ctcttcctat accgatcgcc cgaaacgcta 8521 atcaagtgga cgcgtcccaa ggaggagtac gtggacgatg atggcgacac cgactcgatt 8581 cccagtcgaa tgagcgtgcg ccggccggag caactgcagc cacagcaacc gcaataacat 8641 aggcctaatc caatcgctta actaaagtag agtttgttta cgtatttatt acgtattgcg 8701 ttatcgtagg tttcatagtc ttttttaaac gtttttataa ggagaaagag aacacaaatc 8761 gaaacacaaa agtacgcatc acccatttat acagtcgttc gattttttgt caaaagtact 8821 gcccatgtgt aaaactgtgt atttagtaac taagcgaaaa gtgttgatgt ttgttaaatc 8881 aaacaattgc aaataatagt aaattataat tgcctaatca aggaaataat ttatttaagt 8941 tacttctaaa taagtgtaaa aattgaagat taagtgcgaa aatactaata ataatgatga 9001 aaactcatat tcagggaatt tagcatttta cagaaaccgt aaatataata actaacctaa 9061 agcatccgag ttattaagta aaaatagcga ttaaataata aatgttttat atttatacaa 9121 aaaaaaacaa tatcatgtaa aattagccat tgcttcgatt cgcatacatt tctatgctgt 9181 ttgcctaatt gtgcccttta caactgatta aatatattac gtaaatgtta tactgtttac 9241 tactatataa ctaaatcgat acatattgca tatatagagc gttgacttta gttttaggca 9301 tatcacttga catatttttg tattgaatgc aaatattatt tgcttaaata actcctttct 9361 aggcaaattc atatcaaaat gaaaaaatta aatgatatat agtgcgttgg cttcgaatgg 9421 aaatttctcc cttttgcaat ctcttaaaat tagtaaacgc caactagctc ggtatgtttt 9481 ttttttatat accaaaacga aactaaaatg atgaatgtaa aaaaaaatga atttttatac 9541 taagaatcga acaacttaaa acgtaaatgt aatatgtatg catctatttt tttagcgatt 9601 catttccaaa attagcttat taactgcgtg gaatacttgc gaaat //