TY  - JOUR
AU  - Ebina, Y.
AU  - Ellis, L.
AU  - Jarnagin, K.
AU  - Edery, M.
AU  - Graf, L.
AU  - Clauser, E.
AU  - Ou, J. H.
AU  - Masiarz, F.
AU  - Kan, Y. W.
AU  - Goldfine, I. D.
PY  - DA  - 1985/Apr/
TI  - The human insulin receptor cDNA: the structural basis for hormone-activated transmembrane signalling.
JO  - Cell
SP  - 747
EP  - 758
VL  - 40
IS  - 4
KW  - Amino Acid Sequence
KW  - Base Sequence
KW  - Cell Membrane
KW  - Chromosomes, Human, 19-20
KW  - DNA
KW  - Female
KW  - Humans
KW  - Membrane Proteins
KW  - Nucleic Acid Hybridization
KW  - Placenta
KW  - Poly A
KW  - Pregnancy
KW  - Protein Kinases
KW  - Protein-Tyrosine Kinases
KW  - RNA, Messenger
KW  - Receptor, Epidermal Growth Factor
KW  - Receptor, Insulin
KW  - Receptors, Cell Surface
KW  - Structure-Activity Relationship
KW  - Transcription, Genetic
AB  - A cloned approximately 5 kb cDNA (human placenta) contains the coding sequences for the insulin receptor. The nucleotide sequence predicts a 1382 amino acid precursor. The alpha subunit comprises the N-terminal portion of the precursor and contains a striking cysteine-rich "cross-linking" domain. The beta-subunit (the C-terminal portion of the precursor) contains a transmembrane domain and, in the intracellular region, the elements of a tyrosine phosphokinase: an ATP-binding site and a possible tyrosine autophosphorylation site or sites. The overall structure is reminiscent of the EGF receptor; the cross-linking domain of the alpha subunit and several regions of the beta subunit exhibit sequence homology with the EGF receptor. The phosphokinase domain also exhibits homology with some oncogenic proteins that have tyrosine phosphokinase activity, in particular, a striking homology with v-ros. Southern blotting experiments suggest that the coding region spans more than 45 kb. The insulin receptor gene is located on chromosome 19.
SN  - 0092-8674
UR  - http://www.ncbi.nlm.nih.gov/pubmed/2859121
LA  - eng
ID  - Ebina1985
ER  -