ID   Y09633; SV 4; linear; genomic DNA; STD; PRO; 10486 BP.
XX
AC   Y09633;
XX
DT   08-JAN-1997 (Rel. 50, Created)
DT   18-APR-2005 (Rel. 83, Last updated, Version 11)
XX
DE   Bradyrhizobium japonicum rph, hrcA, grpE, dnaK, dnaJ & pmtA genes
XX
KW   dnaJ gene; dnaK gene; grpE gene; heat shock protein; hrcA gene; pmtA gene;
KW   rph gene.
XX
OS   Bradyrhizobium japonicum
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium.
XX
RN   [1]
RX   DOI; 10.1007/s004380050408.
RX   PUBMED; 9108282.
RA   Minder A.C., Narberhaus F., Babst M., Hennecke H., Fischer H.M.;
RT   "The dnaKJ operon belongs to the sigma32-dependent class of heat shock
RT   genes in Bradyrhizobium japonicum";
RL   Mol. Gen. Genet. 254(2):195-206(1997).
XX
RN   [2]
RC   revised by [4]
RA   Fischer H.M.;
RT   ;
RL   Submitted (27-NOV-1996) to the INSDC.
RL   H.M. Fischer, Mikrobiologisches Institut, ETH-Zentrum, Schmelzbergstrasse
RL   7, CH-8092 Zuerich, SWITZERLAND
XX
RN   [3]
RX   DOI; 10.1128/JB.182.1.14-22.2000.
RX   PUBMED; 10613857.
RA   Minder A.C., Fischer H.M., Hennecke H., Narberhaus F.;
RT   "Role of HrcA and CIRCE in the heat shock regulatory network of
RT   Bradyrhizobium japonicum";
RL   J. Bacteriol. 182(1):14-22(2000).
XX
RN   [4]
RC   revised by [5]
RA   Narberhaus F.;
RT   ;
RL   Submitted (28-SEP-1999) to the INSDC.
RL   F. Narberhaus., Mikrobiologisches Institut, ETH-Zentrum, Schmelzbergstrasse
RL   7, CH-8092 Zuerich, SWITZERLAND
XX
RN   [5]
RP   1-10486
RA   Fischer H.M.;
RT   ;
RL   Submitted (16-MAY-2000) to the INSDC.
RL   H.M. Fischer, Mikrobiologisches Institut, ETH-Zentrum, Schmelzbergstrasse
RL   7, CH-8092 Zuerich, SWITZERLAND
XX
RN   [6]
RX   DOI; 10.1111/j.1365-2958.2001.02325.x.
RX   PUBMED; 11251836.
RA   Minder A.C., de Rudder K.E.E., Narberhaus F., Fischer H.M., Hennecke H.,
RA   Geiger O.;
RT   "Phosphatidylcholine levels in Bradyrhizobium japonicum membranes are
RT   critical for an efficient symbiosis with the soybean host plant";
RL   Mol. Microbiol. 39(5):1186-1198(2001).
XX
DR   EuropePMC; PMC94234; 10613857.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..10486
FT                   /organism="Bradyrhizobium japonicum"
FT                   /strain="110spc4"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:375"
FT   CDS             complement(<1..258)
FT                   /transl_table=11
FT                   /gene="rph"
FT                   /product="RnpH protein"
FT                   /db_xref="InterPro:IPR001247"
FT                   /db_xref="InterPro:IPR020568"
FT                   /db_xref="InterPro:IPR027408"
FT                   /db_xref="UniProtKB/TrEMBL:Q9REF3"
FT                   /protein_id="CAB60663.1"
FT                   /translation="MRPSRRAPDELRPVTLERGVVKYAEGSCLVKFGDTHVLVTATLED
FT                   RLPPWLKGQGRGWVTAEYGMLPRATSERTRREASAGKQSGR"
FT   promoter        complement(294..336)
FT                   /gene="rph"
FT                   /note="putative housekeeping promoter"
FT   promoter        369..412
FT                   /gene="hrcA"
FT                   /note="sigma32-type"
FT   CDS             433..1521
FT                   /transl_table=11
FT                   /gene="hrcA"
FT                   /product="HrcA protein"
FT                   /function="heat shock regulatory protein"
FT                   /db_xref="GOA:Q9REF2"
FT                   /db_xref="InterPro:IPR002571"
FT                   /db_xref="InterPro:IPR011991"
FT                   /db_xref="InterPro:IPR021153"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q9REF2"
FT                   /protein_id="CAB60664.1"
FT                   /translation="MAHHDPIHLIAPRAGLAQLNERSRDIFRQIVESYLATGEPVGSRN
FT                   ISRLIAMPLSPASVRNVMADLEQLGLIYAPHTSAGRLPTELGLRFFVDALMQVGDLNDA
FT                   ERQSIQSQLTSVGQAQSVEAALDQALTRLSGLTRAAAVVLTPKSNARLKHIEFVRLEPE
FT                   KALVILVGEDGQVENRVLTLPPGVPSSAITEAGNFLNSRIRGRTLAEARLELETALGEA
FT                   RAELDQLTQKVISAGIASWSGGENEDRQLIVRGHANLLEDLHALEDLERVRLLFDDLET
FT                   KRGVIDLLGRAETAEGVRIFIGSENKLFSLSGSSTIISPYRDAAGHIVGVLGVIGPTRL
FT                   NYARVIPTVDYAARIVSRLLGG"
FT   promoter        1540..1583
FT                   /gene="grpE"
FT                   /note="sigma32-type"
FT   CDS             1619..2224
FT                   /transl_table=11
FT                   /gene="grpE"
FT                   /product="GrpE protein"
FT                   /function="heat shock protein"
FT                   /db_xref="GOA:Q79V15"
FT                   /db_xref="InterPro:IPR000740"
FT                   /db_xref="InterPro:IPR009012"
FT                   /db_xref="InterPro:IPR013805"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q79V15"
FT                   /protein_id="CAB60665.1"
FT                   /translation="MTDRDRQPEDTTAPTGEPVVSKPYIMPDDPEPGSVELLQKEAAEA
FT                   RDRMLRTLAEMENLRKRTTKEVADARLYGITGFARDVLDIADNLQRALDAVPAEARAAA
FT                   DPGLTSLIEGVELTERSLLNALEKHGVKKFDPQGQKFDPNFQQAMFEVPDASVPAGTVV
FT                   QVMQAGYTIGERVLRPALVGVAKGGAKAASAANSNEVN"
FT   CDS             complement(2358..3080)
FT                   /transl_table=11
FT                   /product="hypothetical protein"
FT                   /note="orf240"
FT                   /db_xref="GOA:Q9REF0"
FT                   /db_xref="InterPro:IPR000868"
FT                   /db_xref="InterPro:IPR006311"
FT                   /db_xref="UniProtKB/TrEMBL:Q9REF0"
FT                   /protein_id="CAB60666.1"
FT                   /translation="MLDRRQVLAALGTSALVALAPTALLAAASIKPDDASALLVIDVQN
FT                   CFLPGGSLAVKEGEQVVPVINKISKAFSNVVMTQDWHTPGHVSFASVHSGKKPFETIDL
FT                   PYGKQVLWPDHCVQGTDGASLSKDLAIPHAELIIRKGFHKDVDSYSAFLEADGKTSTGL
FT                   AGYLKGRKIKRVFVAGLATDFCVAWTALDARKAGFEVYVVEDACRGIDTQGSLAKAWAD
FT                   MAKAGVKRIQSADIAASA"
FT   CDS             complement(3345..4286)
FT                   /transl_table=11
FT                   /product="hypothetical protein"
FT                   /note="orf313"
FT                   /db_xref="UniProtKB/TrEMBL:Q9REE9"
FT                   /protein_id="CAB60667.1"
FT                   /translation="MKLIRPLAALALLAVSALPAFAADAVFPPGLRLGMVPLVGLSTAK
FT                   TFPGFESEDGTVKVLITELPPAAYGEVVSAFNSNPAGTGGVKQDKIETPAGLAYFTTES
FT                   GKAGETAVKRYSMIVPGAGFSGYVAVQIPENATKIYTDEAVRQMFASTVTRKQVSAEEQ
FT                   IALMPFKITDLADFKDIRTLAPGSSIILADGNESTGYESKPFMILGLIGATPQAADDRA
FT                   RFAQEAALQIPGVRESRVTMSEPIRINGQQGFETRIDGVSGKDKTPVTVVQWIRFSSGG
FT                   ASLRIIASAPRDQWQAAFTRFRAVRDGIQPKG"
FT   promoter        4436..4479
FT                   /gene="dnaK"
FT                   /note="minor sigma32-type"
FT   promoter        4544..4584
FT                   /gene="dnaK"
FT                   /note="major sigma32-type"
FT   CDS             4683..6584
FT                   /transl_table=11
FT                   /gene="dnaK"
FT                   /product="DnaK protein"
FT                   /function="heat shock protein"
FT                   /db_xref="GOA:P94317"
FT                   /db_xref="InterPro:IPR012725"
FT                   /db_xref="InterPro:IPR013126"
FT                   /db_xref="InterPro:IPR018181"
FT                   /db_xref="UniProtKB/Swiss-Prot:P94317"
FT                   /protein_id="CAA70846.3"
FT                   /translation="MGKVIGIDLGTTNSCVAVMDGKNAKVIENSEGMRTTPSIVAVTDD
FT                   GERLVGQPAKRQAVTNPERTFFAVKRLIGRRYDDPMVEKDKKLVPYKIVKASNGDAWVE
FT                   ADGQTYSPSQVSAFILQKMKETAEAHLGQKVDQAVITVPAYFNDAQRQATKDAGKIAGL
FT                   EVLRIINEPTAAALAYGLDKTKTGTIAVYDLGGGTFDISILEIGDGVFEVKSTNGDTFL
FT                   GGEDFDMRLVGYLADEFQKEQGINLRNDKLALQRLKEAAEKAKIELSSTTQTEINLPFI
FT                   TADQTGPKHLTMKLTRAKFEALVDDLIQKTVEPCRKALKDAGVTAGEIGEVVLVGGMSR
FT                   MPKVQEVVKQLFGKEPHKGVNPDEVVAIGAAIQAGVLQGDVKDVLLLDVTPLSLGIETL
FT                   GGVFTRIIDRNTTIPTKKSQVFSTAEDNQNAVTIRVFQGEREMAADNKMLGQFDLMGIP
FT                   PAPRGMPQIEVTFDIDANGIVNVSAKDKATGKEQQIRIQASGGLSEADIEKMVKDAEAN
FT                   AEADKKRREAVTAKNEADGLVHSTEKALAEHGSKVAETERRAIEDAVSDLKEALKGDDA
FT                   EAIKAKTQTLAQASMKLGEAMYKQQAEADAKKDAAKDDVVDAEFTEVDDDKNNKKSA"
FT   CDS             6599..6802
FT                   /transl_table=11
FT                   /product="hypothetical protein"
FT                   /note="orf67"
FT                   /db_xref="UniProtKB/TrEMBL:P94318"
FT                   /protein_id="CAA70847.1"
FT                   /translation="MMRTAWTPHTPSASPFAGEAPVSRSAGYAPVTIDQFPAVILNAAM
FT                   LPSAARRRDAYIVAWQHCFAPT"
FT   CDS             6830..7963
FT                   /transl_table=11
FT                   /gene="dnaJ"
FT                   /product="DnaJ protein"
FT                   /function="heat shock protein"
FT                   /db_xref="GOA:P94319"
FT                   /db_xref="InterPro:IPR001305"
FT                   /db_xref="InterPro:IPR001623"
FT                   /db_xref="InterPro:IPR002939"
FT                   /db_xref="InterPro:IPR008971"
FT                   /db_xref="InterPro:IPR012724"
FT                   /db_xref="InterPro:IPR018253"
FT                   /db_xref="UniProtKB/Swiss-Prot:P94319"
FT                   /protein_id="CAA70848.1"
FT                   /translation="MSTSTKRCYYETLEVERDADESKLKSSFRKLAMKFHPDRNPGDDT
FT                   SEVKFKEINEAYEVLKDKDKRAAYDRFGHAAFEQGGPGGGAGFGAGFASSFSDIFEDLF
FT                   GMAGQRGRGGRERGADLRYNMEITLEEAFGGKTAQIEIPVSVTCEACSGIGAKAGTKPK
FT                   TCSTCGGAGRVRQSQGFFTLERTCPGCQGRGQMIEDACPSCSGQGRVTRERTLSVNIPQ
FT                   GVEDGTRIRLAGEGEAGVRGGPPGDLYIFLSLAQHQFFQRDGADLHCRVPISMVTAALG
FT                   GEFEVPTIEKGKAKVKVPAGTQSNRRFRIASKGMPVLRSRQTGDMYVQVVVETPQNLTK
FT                   KQQELLAEFEKLSSGNTQPESEGFFTKVKDFFGSRAN"
FT   CDS             8051..8650
FT                   /transl_table=11
FT                   /gene="pmtA"
FT                   /product="PpmtA protein"
FT                   /db_xref="GOA:Q9LCT2"
FT                   /db_xref="InterPro:IPR001737"
FT                   /db_xref="UniProtKB/TrEMBL:Q9LCT2"
FT                   /protein_id="CAB91878.1"
FT                   /translation="MPLPSSARALKKPRLDDEVRFLRSWIEKPLHMGAVMPSGKLLART
FT                   MAHYVDVNSDAPVVELGPGTGAITSALVERGVDQKRLVLVEYNPGFCALLRDRYPQAKV
FT                   VQGDAYRLRDTLWNVLSAPASAVVSGLPLVTKPMLTRLRLIRDAFTALAPGAPFVQFTY
FT                   AVVPPIPKSLPGVSTEASERIWMNLPPARVWVYRKD"
FT   CDS             8708..9286
FT                   /transl_table=11
FT                   /product="hypothetical protein"
FT                   /note="orf192"
FT                   /db_xref="InterPro:IPR005025"
FT                   /db_xref="UniProtKB/TrEMBL:Q9LCT1"
FT                   /protein_id="CAB91879.1"
FT                   /translation="MSAPKILVIPGSLRTGSHNAKLAALAAYEFAQAGVDVTRISLADF
FT                   PLPIYDGDLQAKSGVPKHAINLKRMIGAHHGVLIVSPEYNASVPPLLKNAIDWVSRVHE
FT                   LHEARGEVFRNRAFALAGASQSRLGAARALQALRLILTSCHANVIASQLTLAFAGEAYD
FT                   DMDRLKNEGDIAALKELVAQLIDISQRMM"
FT   CDS             9293..10006
FT                   /transl_table=11
FT                   /product="hypothetical protein"
FT                   /note="orf237"
FT                   /db_xref="GOA:Q9LCT0"
FT                   /db_xref="InterPro:IPR001754"
FT                   /db_xref="InterPro:IPR011060"
FT                   /db_xref="InterPro:IPR013785"
FT                   /db_xref="InterPro:IPR014732"
FT                   /db_xref="InterPro:IPR018089"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q9LCT0"
FT                   /protein_id="CAB91880.1"
FT                   /translation="MTPAEIAPKDRLIVALDLPSVDAAEAMIARLGDSVTFYKIGYRLA
FT                   YAGGLPLVARLADKGKKVFLDLKLHDIGNTVAQGVESITRLGATFLTVHAYPQTMKGAV
FT                   EGRGGSNLKILAVTVLTSYNEDDLHAAGFRLGVAELVEARAQQAQVLGIDGLVSSPEEV
FT                   GALRKIVGHQMSLVTPGIRPAGSASGDQKRIMTPGRAITAGADYLVVGRPVVEAAEPKA
FT                   IADAIQAEIGQALGA"
FT   CDS             10126..10437
FT                   /transl_table=11
FT                   /product="hypothetical protein"
FT                   /note="orf103"
FT                   /db_xref="InterPro:IPR010753"
FT                   /db_xref="InterPro:IPR011008"
FT                   /db_xref="UniProtKB/TrEMBL:Q9LCS9"
FT                   /protein_id="CAB91881.1"
FT                   /translation="MAKGYWIGRVDVNSDEGYKPYAVANGAIFKKWGGRFVVRGGKFTT
FT                   VEGASRTRNVVIEFPDYETAIACYNSPEYQANIKVRQPHSVADLIIIEGYDGAQPSDG"
XX
SQ   Sequence 10486 BP; 1905 A; 3430 C; 3404 G; 1747 T; 0 other;