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EBI Dbfetch

ID   FP565142; SV 1; linear; mRNA; EST; INV; 778 BP.
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AC   FP565142;
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DT   27-JUL-2010 (Rel. 105, Created)
DT   01-DEC-2010 (Rel. 107, Last updated, Version 3)
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DE   Alvinella pompejana mRNA 5-prime sequence from clone MH0ADA102YA01
DE   (MH0ADA102YA01FM1).
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KW   EST.
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OS   Alvinella pompejana
OC   Eukaryota; Metazoa; Lophotrochozoa; Annelida; Polychaeta; Scolecida;
OC   Terebellida; Alvinellidae; Alvinella.
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RN   [1]
RP   1-778
RA   Genoscope - CEA;
RT   ;
RL   Submitted (27-JUL-2009) to the INSDC.
RL   Genoscope - Centre National de Sequencage : BP 191 91006 EVRY cedex -
RL   FRANCE (E-mail : seqref@genoscope.cns.fr - Web : www.genoscope.cns.fr).
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RN   [2]
RP   1-778
RX   DOI; 10.1186/1471-2164-11-634.
RX   PUBMED; 21080938.
RG   IGBMC, Illkirch, France CEA-Genoscope, CNRS, UMR 8030, Evry, France
RA   Gagniere N., Jollivet D., Boutet I., Brelivet Y., Busso D., Da Silva C.,
RA   Gaill F., Higuet D., Hourdez S., Knoops B., Lallier F., Leize-Wagner E.,
RA   Mary J., Moras D., Perrodou E., Rees J.F., Segurens B., Shillito B.,
RA   Tanguy A., Thierry J.C., Weissenbach J., Wincker P., Zal F., Poch O.,
RA   Lecompte O.;
RT   "Insights into metazoan evolution from Alvinella pompejana cDNAs";
RL   BMC Genomics 11(1):634-634(2010).
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DR   MD5; cf6484c6d6319ad28709769d57dda5c2.
DR   EuropePMC; PMC3018142; 21080938.
DR   EuropePMC; PMC3277501; 22348046.
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CC   The library consists of purified mRNAs using CloneMiner cDNA library
CC   construction kit (Invitrogen) which is designed to construct cDNAlibraries
CC   without traditional restriction enzyme cloning methods. This technology
CC   combines the performance of SuperscriptII reverse Transcriptase with the
CC   Gateway Technology. Single-standed mRNA is converted into double stranded
CC   cDNA containing attB sequences on each end. Through site-specific
CC   recombination, attB-flanked cDNA is cloned directly into attP-containing
CC   donor vector by homologue recombination. Library construction and
CC   sequencing were performed at Genoscope - Centre National de Sequencage
CC   (Evry, FRANCE).
CC   Contact: Lecompte O.
CC   FAX:    +33 (0) 3 88 65 32 76
CC   TEL:    +33 (0) 3 88 65 34 97
CC   EMAIL:  lecompte@igbmc.fr
CC   Laboratoire de Bioinformatique et Genomique Integratives
CC   Institut de Genetique et de Biologie Moleculaire et Cellulaire
CC   1 rue Laurent Fries, BP 10142, 67404 Illkirch CEDEX, France
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FH   Key             Location/Qualifiers
FH
FT   source          1..778
FT                   /organism="Alvinella pompejana"
FT                   /mol_type="mRNA"
FT                   /clone_lib="Alvinella pompejana pygidium library"
FT                   /tissue_type="pygidum"
FT                   /note="Vector:pDONR222"
FT                   /db_xref="taxon:6376"
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SQ   Sequence 778 BP; 249 A; 172 C; 178 G; 179 T; 0 other;
     cgacgcgtct agactaagca gacgatacgc tagcttcaga gattcgcgcc acaaacagcc        60
     gaacaaaatg gcaaacttga aggaaatatt tgatgccatc gacacggacg gaagtggatt       120
     cgtagacaag aaagagttga gagcaatggt taaaacatgc agcgatgctg gtcttctggg       180
     agagaaactg acagatgctc agatcgacga gaccgttgca agcatcatgg aactctgtga       240
     ggacgacaaa cccgatggca agctgtcgtt caaggaattt gagaccggaa tgaacaagct       300
     aacgaagtaa gccgtccagg tgattgccag gcttcttctt attcttctcg tctggaaaca       360
     gaacgcactt ccttgttcat cgtttgaacg gcttcagtat tgcaagtcat gacgcttcag       420
     taatgtcttc acttcaacag aaccgactgt aaataaacaa attcaagcaa gcaagcaaac       480
     aacaacatat aaacaaacaa acaaacacac acacacaaca atacagaaac cagcgacatt       540
     aaactgctcc tgccacgtca caggtcgtct aattatcgct ttacacagag gcttggtttg       600
     ttgaagttgc tattattact aaatagtgcc ggctaggcgt ggatcagctt ccaataggag       660
     agatggtcgt gatggaattg tgttaaaaac atccaagtca ggcacgttgt aatcgtttaa       720
     ctaagtttgt ttcgagccga cgatcgcgtg ctttcatcta agcgagggaa ggtccatc         778
//


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