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EBI Dbfetch

ID   FN257130; SV 1; linear; mRNA; STD; PLN; 422 BP.
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AC   FN257130;
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DT   30-APR-2009 (Rel. 100, Created)
DT   22-AUG-2012 (Rel. 113, Last updated, Version 3)
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DE   Pinus pinaster EST, clone 14-5 JM D5 (MA9-C3-T7)
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KW   .
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OS   Pinus pinaster
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Pinidae; Pinales; Pinaceae; Pinus; Pinus.
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RN   [1]
RP   1-422
RA   Fernandez-Pozo N.;
RT   ;
RL   Submitted (05-MAR-2009) to the INSDC.
RL   Fernandez-Pozo N., Biologia Molecular y Bioquimica, Universidad de Malaga,
RL   Facultad de ciencias, Malaga, 29071, SPAIN.
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RN   [2]
RX   DOI; 10.1186/1471-2164-12-366.
RX   PUBMED; 21762488.
RA   Fernandez-Pozo N., Canales J., Guerrero-Fernandez D., Villalobos D.P.,
RA   Diaz-Moreno S.M., Bautista R., Flores-Monterroso A., Guevara M.A.,
RA   Perdiguero P., Collada C., Cervera M.T., Soto A., Ordas R., Canton F.R.,
RA   Avila C., Canovas F.M., Claros M.G.;
RT   "EuroPineDB: a high-coverage web database for maritime pine transcriptome";
RL   BMC Genomics 12:366-366(2011).
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DR   MD5; e6d27e1ef0a2a4191650d1e446ff3fa4.
DR   EuropePMC; PMC3152544; 21762488.
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CC   The subtractive hybridization protocol from BD Biosciences Clontech (Palo
CC   Alto, CA) was used to generate cDNA libraries 3, 4, 13 and 14.
CC   Differentiating xylem samples for library 3 and 4 were collected from the
CC   compression and opposite sides of a twelve-year-old artificially bent tree
CC   (15 degree away from the vertical position). For library 13 and 14,
CC   juvenile and mature differentiating xylem were sampled from the crown and
CC   the base of a 30-year-old tree. Library 3 (tester: opposite xylem /
CC   driver: compression xylem), library 4 (tester: compression xylem / driver:
CC   opposite xylem), library 13 (tester: juvenile xylem / driver: mature
CC   xylem), library 14 (tester: mature xylem / driver: juvenile xylem).
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FH   Key             Location/Qualifiers
FH
FT   source          1..422
FT                   /organism="Pinus pinaster"
FT                   /ecotype="Corsican"
FT                   /mol_type="mRNA"
FT                   /clone="14-5 JM D5 (MA9-C3-T7)"
FT                   /tissue_type="differentiating xylem"
FT                   /db_xref="taxon:71647"
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SQ   Sequence 422 BP; 111 A; 73 C; 128 G; 110 T; 0 other;
     acgtttgttt gggaagcctg gcagtaatgt ctaatgaaca actcctggaa tttgcttggg        60
     gattggcttc cagcaaccaa tccttcttgt gggttgtgag gccagatata gttcatggtg       120
     aatctgccat attacccaaa gagttcgttg aggaaaccaa ggatagaggt gtgctggtgg       180
     gttgggcgcc tcagataaag gttctatcgc acccatctgt gggaggattt ctaactcaca       240
     gcggttggaa ctccacattg gaaagcatta gtgcgggtgt gccaatgatg tgctggccct       300
     tcttcgccga gcaagaaaca aatgctaaat ttgtgtgtga agagtgggga atcggaatgc       360
     aggtgaagaa aatggtgaag agagaagagt tggcgatact cgtgaggaat ttgatcaagg       420
     gt                                                                      422
//


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