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EBI Dbfetch

ID   FN256437; SV 1; linear; mRNA; STD; PLN; 339 BP.
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AC   FN256437;
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DT   30-APR-2009 (Rel. 100, Created)
DT   22-AUG-2012 (Rel. 113, Last updated, Version 3)
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DE   Pinus pinaster EST, clone 4-2 JM F11 (1)
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KW   .
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OS   Pinus pinaster
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Pinidae; Pinales; Pinaceae; Pinus; Pinus.
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RN   [1]
RP   1-339
RA   Fernandez-Pozo N.;
RT   ;
RL   Submitted (05-MAR-2009) to the INSDC.
RL   Fernandez-Pozo N., Biologia Molecular y Bioquimica, Universidad de Malaga,
RL   Facultad de ciencias, Malaga, 29071, SPAIN.
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RN   [2]
RX   DOI; 10.1186/1471-2164-12-366.
RX   PUBMED; 21762488.
RA   Fernandez-Pozo N., Canales J., Guerrero-Fernandez D., Villalobos D.P.,
RA   Diaz-Moreno S.M., Bautista R., Flores-Monterroso A., Guevara M.A.,
RA   Perdiguero P., Collada C., Cervera M.T., Soto A., Ordas R., Canton F.R.,
RA   Avila C., Canovas F.M., Claros M.G.;
RT   "EuroPineDB: a high-coverage web database for maritime pine transcriptome";
RL   BMC Genomics 12:366-366(2011).
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DR   MD5; 8272b31124e85aa9da654c12f629a8eb.
DR   EuropePMC; PMC3152544; 21762488.
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CC   The subtractive hybridization protocol from BD Biosciences Clontech (Palo
CC   Alto, CA) was used to generate cDNA libraries 3, 4, 13 and 14.
CC   Differentiating xylem samples for library 3 and 4 were collected from the
CC   compression and opposite sides of a twelve-year-old artificially bent tree
CC   (15 degree away from the vertical position). For library 13 and 14,
CC   juvenile and mature differentiating xylem were sampled from the crown and
CC   the base of a 30-year-old tree. Library 3 (tester: opposite xylem /
CC   driver: compression xylem), library 4 (tester: compression xylem / driver:
CC   opposite xylem), library 13 (tester: juvenile xylem / driver: mature
CC   xylem), library 14 (tester: mature xylem / driver: juvenile xylem).
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FH   Key             Location/Qualifiers
FH
FT   source          1..339
FT                   /organism="Pinus pinaster"
FT                   /ecotype="Corsican"
FT                   /mol_type="mRNA"
FT                   /clone="4-2 JM F11 (1)"
FT                   /tissue_type="differentiating xylem"
FT                   /db_xref="taxon:71647"
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SQ   Sequence 339 BP; 108 A; 72 C; 81 G; 78 T; 0 other;
     acctggatac agcagcagga aagcattggc agacaaccag gcattacaag ttcaatgtga        60
     ggcagagtaa tgtgacacgt ctttgcaaca caaaaccttt gatcaccgtg aatggacaat       120
     atccaggacc tactattttt gctcaggagg gagatcaagt aattataaaa ctggtaaacc       180
     acgtcaagga caacgtgact attcactggc atgggattcg acagctgaga tctggatggg       240
     cagatggtcc tggttatatc acccaatgtc cacttcaaac gggaatgagt tacgtttata       300
     acttcaccat cacaggacag agaggaactc tatggtggc                              339
//


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