Dbfetch

ID   BX784385; SV 1; linear; mRNA; EST; PLN; 553 BP.
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AC   BX784385;
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DT   12-NOV-2003 (Rel. 77, Created)
DT   12-NOV-2003 (Rel. 77, Last updated, Version 1)
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DE   Pinus pinaster xylem EST, clone 133H11 similar to Basic blue protein
DE   (Cusacyanin) (Plantacyanin)
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KW   EST; expressed sequence tag.
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OS   Pinus pinaster
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Pinidae; Pinales; Pinaceae; Pinus; Pinus.
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RN   [1]
RP   1-553
RA   Frigerio J.M.;
RT   ;
RL   Submitted (12-NOV-2003) to the INSDC.
RL   Frigerio J.M. UMR BIOGECO INRA, Genetique et Amelioration 69, route
RL   d'Arcachon 33612 Cestas CEDEX France
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RN   [2]
RA   Canton F.R., Le Provost G., Garcia V., Barre A., Frigerio J-M., Paiva J.,
RA   Fevereiro P., Avila C., Mouret J-F., Brach J., de Daruvar A., Canovas F.M.,
RA   Plomion C.;
RT   "TRANSCRIPTOME ANALYSIS OF WOOD FORMATION IN MARITIME PINE";
RL   Unpublished.
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DR   MD5; f2c8acb57d7affd7353ef7d851da91ac.
DR   EuropePMC; PMC3152544; 21762488.
DR   UNILIB; 30130; 12700.
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CC   Email: Frigerio@pierroton.inra.fr
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FH   Key             Location/Qualifiers
FH
FT   source          1..553
FT                   /organism="Pinus pinaster"
FT                   /mol_type="mRNA"
FT                   /dev_stage="adult"
FT                   /clone="133H11"
FT                   /tissue_type="differenciating xylem"
FT                   /note="ecotype: Corsican"
FT                   /note="Vector: Uni-Zap XR lambda (Stratagene); Site_1: Eco
FT                   RI; Site_2: Xho I. A composite cDNA library was made with
FT                   mRNA isolated from normal, compression, opposite, early and
FT                   late wood of Maritime pine uni-directionally cloned into
FT                   Uni-ZAP XR using the ZAP-cDNA Synthesis kit (Stratagene).
FT                   pBluescript SK(-) plasmids were obtained by in vivo mass
FT                   excision. The nucleotide sequence of the 5'end was obtained
FT                   by automated sequencing with the T3 primer by GENOME
FT                   EXPRESS, Meylan, France"
FT                   /db_xref="taxon:71647"
FT                   /db_xref="UNILIB:30130"
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SQ   Sequence 553 BP; 152 A; 123 C; 124 G; 154 T; 0 other;
     atggaagcag ctgctaatat ttttggcatc tcattcatgg caaacactac tgtgatgctc        60
     ttgttgatga tcctagcatt ggccataaca cagagctgta tggctgtgca gtatgctgtt       120
     ggaggcagcc aaggatggga tctcagtact gacctcagta catgggaatc tagcaaaact       180
     ttcaaagttg gagacgccct atctttcaag tacacagcgg ggttgcacag tgtggtggag       240
     cttgcaagtg agaaagatta caactcatgt aatattggga atccagtgaa cagtttgagt       300
     ggaggaagca atgttgtgaa gctaagtaag gcagggacaa ggtattttgc ttgcggaaca       360
     ccagggcatt gctctggagg catgaagatg aaaatcaaag ttgtggctgc aggaacttca       420
     cctacacctt catcaacacc ttcatccaaa aatccttcat ccacatcatc atcttcctct       480
     tccacttccc tctgcaataa aattctcccc tgcatctcat attggcttga tcttcataag       540
     ctgcttctct tta                                                          553
//