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EBI Dbfetch

ID   BX784033; SV 1; linear; mRNA; EST; PLN; 272 BP.
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AC   BX784033;
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DT   07-NOV-2003 (Rel. 77, Created)
DT   07-NOV-2003 (Rel. 77, Last updated, Version 1)
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DE   Pinus pinaster xylem EST, clone 121D10 similar to Pyrophosphate--fructose
DE   6-phosphate 1-phosphotransferase beta subunit (EC 2.7.1.90)
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KW   EST; expressed sequence tag.
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OS   Pinus pinaster
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Pinidae; Pinales; Pinaceae; Pinus; Pinus.
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RN   [1]
RP   1-272
RA   Frigerio J.M.;
RT   ;
RL   Submitted (05-NOV-2003) to the INSDC.
RL   Frigerio J.M. UMR BIOGECO INRA, Genetique et Amelioration 69, route
RL   d'Arcachon 33612 Cestas CEDEX France
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RN   [2]
RA   Canton F.R., Le Provost G., Garcia V., Barre A., Frigerio J-M., Paiva J.,
RA   Fevereiro P., Avila C., Mouret J-F., Brach J., de Daruvar A., Canovas F.M.,
RA   Plomion C.;
RT   "TRANSCRIPTOME ANALYSIS OF WOOD FORMATION IN MARITIME PINE";
RL   Unpublished.
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DR   MD5; efc7def935f405d5cbb699fe8dc6806c.
DR   EuropePMC; PMC3152544; 21762488.
DR   UNILIB; 30130; 12700.
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CC   Email: Frigerio@pierroton.inra.fr
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FH   Key             Location/Qualifiers
FH
FT   source          1..272
FT                   /organism="Pinus pinaster"
FT                   /mol_type="mRNA"
FT                   /dev_stage="adult"
FT                   /clone="121D10"
FT                   /tissue_type="differenciating xylem"
FT                   /note="ecotype: Corsican"
FT                   /note="Vector: Uni-Zap XR lambda (Stratagene); Site_1: Eco
FT                   RI; Site_2: Xho I. A composite cDNA library was made with
FT                   mRNA isolated from normal, compression, opposite, early and
FT                   late wood of Maritime pine uni-directionally cloned into
FT                   Uni-ZAP XR using the ZAP-cDNA Synthesis kit (Stratagene).
FT                   pBluescript SK(-) plasmids were obtained by in vivo mass
FT                   excision. The nucleotide sequence of the 5'end was obtained
FT                   by automated sequencing with the T3 primer by GENOME
FT                   EXPRESS, Meylan, France"
FT                   /db_xref="taxon:71647"
FT                   /db_xref="UNILIB:30130"
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SQ   Sequence 272 BP; 83 A; 58 C; 49 G; 82 T; 0 other;
     tttttttttt ttttttttac caaatgaatg caaaatttta tttgaaattt gacaagcatg        60
     ccacctttcc aaaaggttgg ctaagctatc aacgtttcag tgttcaccca taaccagagg       120
     gcggacgaga atgacaaact aacgagaaaa tgttaccaag catctacttc aatcatgagc       180
     ttcgttcaac caacagtgca ttcgggagaa attcctgaat ccttgaagta acatggttcc       240
     tcgggcatgc tctgaacttc tagagtcaag cc                                     272
//


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