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EBI Dbfetch

ID   BX682240; SV 2; linear; mRNA; EST; PLN; 435 BP.
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AC   BX682240;
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DT   30-OCT-2003 (Rel. 77, Created)
DT   30-OCT-2003 (Rel. 77, Last updated, Version 1)
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DE   Pinus pinaster xylem EST, clone 114A02 similar to Adenosine kinase (EC
DE   2.7.1.20)
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KW   EST; expressed sequence tag.
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OS   Pinus pinaster
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Pinidae; Pinales; Pinaceae; Pinus; Pinus.
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RN   [1]
RP   1-435
RA   Frigerio J.M.;
RT   ;
RL   Submitted (30-NOV-2003) to the INSDC.
RL   Frigerio J.M. UMR BIOGECO INRA, Genetique et Amelioration 69, route
RL   d'Arcachon 33612 Cestas CEDEX France
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RN   [2]
RA   Canton F.R., Le Provost G., Garcia V., Barre A., Frigerio J-M., Paiva J.,
RA   Fevereiro P., Avila C., Mouret J-F., Brach J., de Daruvar A., Canovas F.M.,
RA   Plomion C.;
RT   "TRANSCRIPTOME ANALYSIS OF WOOD FORMATION IN MARITIME PINE";
RL   Unpublished.
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DR   MD5; 26d7ce262991911e5d1f162fb22ff0c4.
DR   EuropePMC; PMC3152544; 21762488.
DR   UNILIB; 30130; 12700.
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CC   Email: Frigerio@pierroton.inra.fr
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FH   Key             Location/Qualifiers
FH
FT   source          1..435
FT                   /organism="Pinus pinaster"
FT                   /mol_type="mRNA"
FT                   /dev_stage="adult"
FT                   /clone="114A02"
FT                   /tissue_type="differenciating xylem"
FT                   /note="ecotype: Corsican"
FT                   /note="Vector: Uni-Zap XR lambda (Stratagene); Site_1: Eco
FT                   RI; Site_2: Xho I. A composite cDNA library was made with
FT                   mRNA isolated from normal, compression, opposite, early and
FT                   late wood of Maritime pine uni-directionally cloned into
FT                   Uni-ZAP XR using the ZAP-cDNA Synthesis kit (Stratagene).
FT                   pBluescript SK(-) plasmids were obtained by in vivo mass
FT                   excision. The nucleotide sequence of the 5'end was obtained
FT                   by automated sequencing with the T3 primer by GENOME
FT                   EXPRESS, Meylan, France"
FT                   /db_xref="taxon:71647"
FT                   /db_xref="UNILIB:30130"
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SQ   Sequence 435 BP; 133 A; 67 C; 110 G; 125 T; 0 other;
     tacagtctca cctgagtcga ttcaacttgt ggctgaacat gcagctcaga aagggaagat        60
     atttatgatg aatcttgcag ctccctttat ctgtgaattt tttaccgagg cattgatgaa       120
     agtttttccg tatatggatt atgtttttgg aaatgagact gaagcaagga cttttgcaaa       180
     ggcgcaaggg tgggagactg acaatgttga agaaatagcc ctaaagatgt cagcactgcc       240
     aaaggcaact ggaactcaca agaggatagc tgttattaca caaggtgcag atccagtagt       300
     cattgcagaa gatggaaagg ttacgttgtt tccagtgatt ttgattccga aggaaaatat       360
     tgttgataca aatggagcag gagatgcatt tgtaggaggc tttttagctc aattggtgct       420
     aggtaaaact attga                                                        435
//


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