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GO:0002143 wobble position uridine thiolation

The process whereby a uridine residue at position 34 in the anticodon of a tRNA is post-transcriptionally thiolated at the C2 position. This process involves transfer of a sulfur from cysteine to position C2 by several steps.

Term Information

ID GO:0002143
Name wobble position uridine thiolation
Ontology Biological Process
Definition The process whereby a uridine residue at position 34 in the anticodon of a tRNA is post-transcriptionally thiolated at the C2 position. This process involves transfer of a sulfur from cysteine to position C2 by several steps.
Comment In E. coli, the first step of the reaction is reductive elimination of sulfur from L-cysteine by IscS cysteine desulfurase to form an enzyme-bound cysteine-persulfide intermediate. Then, five essential gene products, TusA, TusB, TusC, TusD and TusE, mediate a sulfur relay that delivers the terminal sulfur of persulfide from IscS to MnmA12. The last protein, MnmA catalyzes the transfer of the sulfur from IscS to an ATP activated U34 of the tRNA.

Synonyms

Type Synonym
exact tRNA wobble uridine thiolation
exact wobble position s2U biosynthesis

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GO:0008150 biological_process
GO:0008152 metabolic process
GO:0009987 cellular process
GO:0006807 nitrogen compound metabolic process
GO:0044238 primary metabolic process
GO:0043170 macromolecule metabolic process
GO:0044237 cellular metabolic process
GO:0043283 biopolymer metabolic process
GO:0010467 gene expression
GO:0043412 biopolymer modification
GO:0044260 cellular macromolecule metabolic process
GO:0006139 nucleobase, nucleoside, nucleotide and nucleic acid metabolic process
GO:0034960 cellular biopolymer metabolic process
GO:0016070 RNA metabolic process
GO:0034660 ncRNA metabolic process
GO:0006396 RNA processing
GO:0006399 tRNA metabolic process
GO:0009451 RNA modification
GO:0034470 ncRNA processing
GO:0008033 tRNA processing
GO:0006400 tRNA modification
GO:0002097 tRNA wobble base modification
GO:0034227 tRNA thio-modification
GO:0002098 tRNA wobble uridine modification
GO:0002143 wobble position uridine thiolation

Relationship key

Code Relationship
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Child Terms

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Protein Annotation

DB
ID
Qualifier colocalizes_with contributes_to NOT NOT|colocalizes_with NOT|contributes_to
GO Term
Reference
Evidence IMP IGI IPI IGC ISS IDA IEP IEA TAS NAS ND IC RCA ISA EXP
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Taxonomy
From
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Co-occurring Terms

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